A general method to regenerate arrayed gold microelectrodes for label-free cell assay

Xu, Zhihui; Shi, Xueyan; Jiang, Huijun; Song, Yiyan; Zhang, Liying; Wang, Fangyuan; Du, Shouying; Chen, Jin

doi:10.1016/j.ab.2016.10.012

Cited by 11 publications

(13 citation statements)

References 22 publications

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“…Further encapsulation and release of the anti-cancer drug of Dox by mesoporous materials caused a dramatic decrease of the growth curve of A549. These experimental results showed consistency with using fresh electronic plates and with previously reported work 19 , which verified the efficiency of this proposed regeneration protocol.…”

Section: Representative Resultssupporting

confidence: 91%

“…The optimization of trypsinization, including concentration and time, should be the first priority to achieve regeneration. Besides the digestion time that is rationalized in our previous report 19 , a good assessment for the optimization process is simply to check the surface properties using an optical microscope.…”

Section: Discussionmentioning

confidence: 99%

“…Please click here to view a larger version of this figure. Figure 5A has been modified fromXu, Z. et al 19 Please click here to view a larger version of this figure.…”

Section: Figurementioning

confidence: 99%

“…(C) Regenerated electronic plates (D) A549 cells were plated on C.In the case of cells attached on gold microchips, DOX was used. Figure2Aand Figure2Chave been modified from Xu, Z. et al19 Please click here to view a larger version of this figure.…”

mentioning

confidence: 99%

“…(B) Cytotoxicity test of mesoporous silica materials on electronic plate L8 with error bars (standard deviation, STD) of recorded curves of each incubation well. Figure 4A has been modified from Xu, Z. et al19 Please click here to view a larger version of this figure.…”

mentioning

confidence: 99%

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Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Song

Jiang

et al. 2018

JoVE

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The label-free cell-based assay is advantageous for biochemical study because of it does not require the use of experimental animals. Due to its ability to provide more dynamic information about cells under physiological conditions than classical biochemical assays, this label-free real-time cell assay based on the electric impedance principle is attracting more attention during the past decade. However, its practical utilization may be limited due to the relatively expensive cost of measurement, in which costly consumable disposable gold microchips are used for the cell analyzer. In this protocol, we have developed a general strategy to regenerate arrayed gold microelectrodes equipped for a commercial label-free cell analyzer. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step. The proposed method has been tested and shown to be effective for the regeneration and repeated usage of commercial electronic plates at least three times, which will help researchers save on the high running cost of real-time cell assays.

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Section: Representative Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

“…Please click here to view a larger version of this figure. Figure 5A has been modified fromXu, Z. et al 19 Please click here to view a larger version of this figure.…”

Section: Figurementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Song

Jiang

et al. 2018

JoVE

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Real-time cell analysis of the cytotoxicity of a pH-responsive drug-delivery matrix based on mesoporous silica materials functionalized with ferrocenecarboxylic acid

Cai

Jiang

et al. 2019

Analytica Chimica Acta

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A general method to regenerate arrayed gold microelectrodes for label-free cell assay

Cited by 11 publications

References 22 publications

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Real-time cell analysis of the cytotoxicity of a pH-responsive drug-delivery matrix based on mesoporous silica materials functionalized with ferrocenecarboxylic acid

Real-time cell analysis system in cytotoxicity applications: Usefulness and comparison with tetrazolium salt assays

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