Real-time cell analysis of the cytotoxicity of a pH-responsive drug-delivery matrix based on mesoporous silica materials functionalized with ferrocenecarboxylic acid

Xu, Zhihui; Cai, Ling; Jiang, Huijun; Wen, Yuqing; Peng, Luming; Wu, Yuan; Chen, Jin

doi:10.1016/j.aca.2018.11.017

Cited by 15 publications

(10 citation statements)

References 61 publications

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“…The DOX loading content in DOX/SBA-15 and DOX/FeIX-SBA-15 was calculated to be 1.14% and 4.27%, respectively. The results indicated that FeIXgrafted SBA-15 enhanced the load capability of DOX in the mesopores which was ~ 3.7-fold of DOX/SBA-15 and ~ 1.6-fold of that of DOX/FCA-SBA-15 from our earlier work [24]. Such improved loading capability of drug molecules could be ascribed to the refined molecular interactions between FeIX and DOX.…”

Section: Materials Characterizationsmentioning

confidence: 54%

“…As shown in Fig. 1C, all the samples exhibited typical type IV isotherms with a sharp capillary condensation step at high relative pressures, indicating the retaining of mesostructure after chemical modifications [24]. As shown in Fig.…”

Section: Materials Characterizationsmentioning

confidence: 67%

“…In this study, we successfully grafted hemin on the SBA-15 to build a composite material (FeIX-SBA-15) (Scheme 1), in which not only the enzyme-like activity of hemin was retained, but the efficient encapsulation and sustained release of doxorubicin hydrochloride (DOX) were achieved as reflected by the growth curve of incubated cancer cells by the real-time cell analyzer (RTCA) technology [21][22][23]. Notably, a relatively high loading content of DOX was obtained for DOX/FeIX-SBA-15 as compared with our earlier work using ferrocenecarboxylic acid-modified SBA-15 (FCA-SBA-15) [24] which may be due to the refined π-π stacking between grafted FeIX and DOX in the support. In addition, owing to the solid form of FeIX-SBA-15 that was immobilized on a commercial filter membrane, a flow catalysis format for effective dye degradation and colorimetric detection of hydrogen peroxide was developed.…”

Section: Introductionmentioning

confidence: 85%

“…Then, the molecules of FeIX were grafted on the surface of APTES-SBA-15 through an amide reaction and electrostatic interaction between the carboxyl groups and the amino groups in the mesopore. Finally, the anticancer drug DOX loaded into FeIX-SBA-15 composites involving the strong molecular interactions of π-π stacking between FeIX and DOX due to the conjugated planar macrocyclic molecule of FeIX [25] and the anthracycline chromophore of DOX [24].…”

Section: Materials Characterizationsmentioning

confidence: 99%

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Porphyrin Iron-Grafted Mesoporous Silica Composites for Drug Delivery, Dye Degradation and Colorimetric Detection of Hydrogen Peroxide

Zhu

Cai

et al. 2021

Nanoscale Res Lett

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Porphyrin iron molecules (hemin) were successfully grafted on the channeled mesoporous silica of SBA-15 (FeIX-SBA-15), in which attached hemin molecules acted as the enzyme mimic for catalyzing oxidation reactions. In the presence of H2O2, the prepared FeIX-SBA-15 composite effectively degraded industrial dye Orange II and catalyzed tetramethylbenzidine hydrochloride (TMB) both in the solution and on the membrane, from which the colorimetric H2O2 detection was achieved. Moreover, the hemin-grafted composites showed high loading content of anticancer drug of doxorubicin hydrochloride (DOX) displaying the sustained releasing behavior as monitored by real-time cell analysis, which resulted in improved inhibitory effect on cancer cells growth compared with that DOX/SBA-15. The hemin-modified mesoporous silica nanocomposite provides an integrated nanoplatform with promising biomedical applications.

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Section: Materials Characterizationsmentioning

confidence: 54%

Section: Materials Characterizationsmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 85%

Section: Materials Characterizationsmentioning

confidence: 99%

See 2 more Smart Citations

Porphyrin Iron-Grafted Mesoporous Silica Composites for Drug Delivery, Dye Degradation and Colorimetric Detection of Hydrogen Peroxide

Zhu

Cai

et al. 2021

Nanoscale Res Lett

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“…Recently, RTCA holds promising potential in cellular assays because of the advantages of recording signals in a dynamic and label-free mode. In a typical run, upon cells' incubation on the arrayed gold microchips, the produced electrical impendence reflecting the physiological status of cells such as cell proliferation and viability was continuously monitored (Xu et al, 2017;Xu et al, 2018;Xu et al, 2019). Without the use of labeled dyes, RTCA permits a direct and continuous measurement of cells under physiological conditions (Otero-Gonzalez et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Comparison of cytotoxicity evaluation of chlorogenic acid extract between Real-time cell analysis and CCK-8 method

2021

Ukr. Jour. of Vet. and Agr. Sci.

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Critical cytotoxicity evaluation of pharmaceuticals is necessary for the clinical practice of chemotherapy. To quantitatively evaluate cell viability, currently there are two main types of sensitive methods including real-time cell analysis (RTCA) and CCK-8 assay, in which RTCA records electrochemical signal changes around an incubated cell, whereas CCK-8 is based on the colorimetric method. Despite the different detection principles adopted for the cytotoxicity assessment, the comparison of the two methods in terms of the application scope is lacking. In order to compare and determine the best experimental method for the study of the toxicity of chlorogenic acid extract from taraxacum officinale on dairy cow mammary epithelial cells. The real time cell analysis (RTCA) and CCK-8 method were used to analyze the cytotoxicity of chlorogenic acid extract to BMEC and calculate its IC50. The results of the real time cell analysis method and the CCK-8 method showed that different concentrations of chlorogenic acid extract reduced the viability of dairy cow mammary epithelial cells, and the decrease was most obvious at 400 ug/mL. The IC50 of the two analysis methods were 326.8 and 320.4 ug/mL, respectively. In contrast, the CCK-8 method had limitations in fixed-point determination. However, the real time cell analysis method can monitor the dynamic biological response process of cell growth and proliferation in real time. Therefore, the real time cell analysis method can observe cell growth more intuitively and accurately, compensate for the shortcomings of the CCK-8 method, and it is a new experimental method for studying cytotoxicity.

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A Tumoricidal Lipoprotein Complex Electrostatically Stabilized on Mesoporous Silica as Nanotherapeutics and Nanoadjuvant for Potentiating Immunotherapy of Triple Negative Breast Cancer

Pei,

Li,

et al. 2023

Adv Funct Materials

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As the most aggressive subtype, triple‐negative breast cancer (TNBC) without definitive targets represents a high probability of metastasis. Nevertheless, the presence of high‐level tumor‐infiltrating lymphocytes in the TNBC tumor microenvironment (TME) suggests patients may benefit from immunotherapy. In this study, bovine α‐lactalbumin coupled with oleic acid that forms tumoricidal lipid–protein complex (BAMLET) is electrostatically stabilized on the surface of amino‐functionalized mesoporous silica nanoparticles (MSN‐NH2). It is found that MSN‐NH2 may cause partial conformational changes of immobilized proteins due to electrostatic interactions. Therefore, BAMLET covered MSN‐NH2 (BMSN) as a drug cargo that can not only induce selectively oncolytic effect but kill tumor cells with rapid kinetics. Moreover, the hemolytic activity of BMSN conjugated with pre‐formed serum protein corona is largely reduced which may avoid the poor hemocompatibility caused by BAMLET. Furthermore, the in vivo study indicates that the combinational use of drug‐loaded BMSN and immune checkpoint small‐molecule inhibitor can efficiently inhibit the growth of solid TNBC tumors and activate immune response by sufficient infiltration of immune cells in the TME and prevent the seeding of circulating tumor cells. Therefore, the constructed BMSN provides an integrated nanotherapeutics and a nanoadjuvant with superior anticancer performance to combat TNBC.

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Real-time cell analysis of the cytotoxicity of a pH-responsive drug-delivery matrix based on mesoporous silica materials functionalized with ferrocenecarboxylic acid

Cited by 15 publications

References 61 publications

Porphyrin Iron-Grafted Mesoporous Silica Composites for Drug Delivery, Dye Degradation and Colorimetric Detection of Hydrogen Peroxide

Porphyrin Iron-Grafted Mesoporous Silica Composites for Drug Delivery, Dye Degradation and Colorimetric Detection of Hydrogen Peroxide

Comparison of cytotoxicity evaluation of chlorogenic acid extract between Real-time cell analysis and CCK-8 method

A Tumoricidal Lipoprotein Complex Electrostatically Stabilized on Mesoporous Silica as Nanotherapeutics and Nanoadjuvant for Potentiating Immunotherapy of Triple Negative Breast Cancer

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