Recombinant fetuin-B protein maintains high fertilization rate in cumulus cell-free mouse oocytes

Dietzel, Eileen; Floehr, Julia; Leur, Eddy Van de; Weiskirchen, Ralf; Jahnen‐Dechent, Willi

doi:10.1093/molehr/gaw067

Cited by 19 publications

(20 citation statements)

References 39 publications

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“…Because prolonged in vitro culture can decrease the fertilization rate by increasing ZP hardening, this phenomenon is especially crucial when assisted reproductive techniques are used. Under these circumstances, serum glycoprotein fetuin‐B, a cystatin superfamily metalloprotease inhibitor that is essential for mouse fertility (Dietzel et al, 2013), was shown to maintain in vitro fertilization by counteracting ovastacin‐mediated cleavage of ZP2 (Dietzel, Floehr, van de Leur, Weiskirchen, & Jahnen‐Dechent, 2017; Dietzel et al, 2013; Floehr et al, 2016). A recent crystallographic study of fetuin‐B and its complex with crayfish astacin, a nonphysiological binding partner that resembles ovastacin, suggested that the function of the inhibitor depends on a rigid disulfide‐linked CPDCP motif located between its two cystatin‐type modules (Cuppari et al, 2019; Figure 2).…”

Section: Cg Exocytosis Modifies the Zp After Fertilizationmentioning

confidence: 99%

“…mediated cleavage of ZP2(Dietzel, Floehr, van de Leur, Weiskirchen, & Jahnen-Dechent, 2017;Dietzel et al, 2013;Floehr et al, 2016). A recent crystallographic study of fetuin-B and its complex with crayfish astacin, a nonphysiological binding partner that resembles ovastacin, suggested that the function of the inhibitor depends on a rigid disulfide-linked CPDCP motif located between its two cystatin-type modules(Cuppari et al, 2019; Figure 2).…”

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confidence: 99%

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Mammalian egg coat modifications and the block to polyspermy

Fahrenkamp

Algarra

Jovine

2020

Molecular Reproduction Devel

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Fertilization by more than one sperm causes polyploidy, a condition that is generally lethal to the embryo in the majority of animal species. To prevent this occurrence, eggs have developed a series of mechanisms that block polyspermy at the level of the plasma membrane or their extracellular coat. In this review, we first introduce the mammalian egg coat, the zona pellucida (ZP), and summarize what is currently known about its composition, structure, and biological functions. We then describe how this specialized extracellular matrix is modified by the contents of cortical granules (CG), secretory organelles that are exocytosed by the egg after gamete fusion. This process releases proteases, glycosidases, lectins and zinc onto the ZP, resulting in a series of changes in the properties of the egg coat that are collectively referred to as hardening. By drawing parallels with comparable modifications of the vitelline envelope of nonmammalian eggs, we discuss how CG‐dependent modifications of the ZP are thought to contribute to the block to polyspermy. Moreover, we argue for the importance of obtaining more information on the architecture of the ZP, as well as systematically investigating the many facets of ZP hardening.

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Section: Cg Exocytosis Modifies the Zp After Fertilizationmentioning

confidence: 99%

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confidence: 99%

Mammalian egg coat modifications and the block to polyspermy

Fahrenkamp

Algarra

Jovine

2020

Molecular Reproduction Devel

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“…Although the physiological roles of fetuin-B in the ovulation system have not been elucidated yet, based on these observations, it is reasonable to speculate that fetuin-B may contribute to the development of PCOS independently. In addition, the relation of fetuin-B and reproductive system has been reported [11,19]. In a study by Fang et al, they reported that fetuin-B levels in serum and follicular fluid were correlated with fertilization rate in IVF cycles [19].…”

Section: Discussionmentioning

confidence: 99%

“…Fetuin-B increases migration of monocytes and macrophages in the vascular plaque and it also increases vascular plaque vulnerable [10]. In addition, the role of fetuin-B in reproductive system has also been reported [11]. Due to the lack of sufficient data regarding to the link between fetuin-B and PCOS, we aimed to discover altered levels of fetuin-B in PCOS women.…”

Section: Increased Fetuin-b Levels Are Associated With Carotid Intimamentioning

confidence: 98%

Increased fetuin-B levels are associated with carotid intima media thickness and hormonal-metabolic disturbances in women with polycystic ovary syndrome

Bayındır¹,

Ünal²,

İmamoğlu³

et al. 2020

Zeynep Kamil Tıp Bülteni

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Objective: Fetuin-B is a novel peptide hormone involves in energy metabolism, atherosclerosis and reproductive system. Polycystic ovary syndrome (PCOS) is a reproductive and metabolic disorder associated with increased cardiovascular risk factors. We aimed to ascertain whether circulating fetuin-B levels were altered in women with PCOS and whether there was any association between fetuin-B and hormonal-metabolic parameters as well as carotid intima media thickness (CIMT). Material and Methods: Eighty women with PCOS and 80 ageand BMI-matched controls without PCOS were recruited to this cross-sectional study. Circulating fetuin-B levels were measured by ELISA. Metabolic-hormonal parameters and CIMT of recruited subjects were also determined. Results: Circulating fetuin-B levels were significantly elevated in women with PCOS compared to controls (409.54 ± 32.51 vs. 325.53 ± 26.86 ng/ml, P<0.001). PCOS subjects with insulin resistance showed significantly elevated circulating fetuin-B levels compared to those PCOS subjects without insulin resistance (416.03 ± 30.41 vs. 399.27 ± 33.56 ng/ml, P = 0.024). Overweight/obese women had significantly higher circulating fetuin-B levels than lean subjects in PCOS groups (416.32 ± 30.99 vs. 400.82 ± 32.77 ng/ml, P = 0.033). Fetuin-B levels positively correlated with BMI, homeostasis model assessment of insulin resistance (HOMA-IR), free-androgen index (FAI), high sensitivity C-reactive protein (hs-CRP), triglycerides and CIMT in PCOS subjects whereas fetuin-B negatively correlated with high density lipoprotein cholesterol (HDL-C). Moreover, binary logistic regression analysis revealed that the risk of having PCOS was increased in parallel with elevation of fetuin-B levels. Conclusion: Circulating fetuin-B levels were significantly increased in PCOS subjects and fetuin-B levels were closely related to cardiovascular risk and hormonal-metabolic disturbances in women with PCOS.

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“…The finding that fertilization can be controlled by a liver-derived plasma protein demonstrates extreme complexity of the regulatory circuits orchestrating mammalian fecundity. Based on the mice data, the authors proposed that the addition of exogenous fetuin-B into the oocyte culture media might increase in vitro fertilization (IVF) success in humans [129]. So far, only two clinical studies assessed the association between the level of fetuin-B and fertilization rate in human IVF patients.…”

Section: Cortical Reactionmentioning

confidence: 99%

Perfect date—the review of current research into molecular bases of mammalian fertilization

Trebichalská

Holubcová

2020

J Assist Reprod Genet

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Fertilization is a multistep process during which two terminally differentiated haploid cells, an egg and a sperm, combine to produce a totipotent diploid zygote. In the early 1950s, it became possible to fertilize mammalian eggs in vitro and study the sequence of cellular and molecular events leading to embryo development. Despite all the achievements of assisted reproduction in the last four decades, remarkably little is known about the molecular aspects of human conception. Current fertility research in animal models is casting more light on the complexity of the process all our lives start with. This review article provides an update on the investigation of mammalian fertilization and highlights the practical implications of scientific discoveries in the context of human reproduction and reproductive medicine.

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Recombinant fetuin-B protein maintains high fertilization rate in cumulus cell-free mouse oocytes

Cited by 19 publications

References 39 publications

Mammalian egg coat modifications and the block to polyspermy

Mammalian egg coat modifications and the block to polyspermy

Increased fetuin-B levels are associated with carotid intima media thickness and hormonal-metabolic disturbances in women with polycystic ovary syndrome

Perfect date—the review of current research into molecular bases of mammalian fertilization

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