Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus

Brielle, Régine; Pinel-Marie, Marie-Laure; Chat, Sophie; Gillet, Reynald; Felden, Brice

doi:10.1016/j.ymeth.2016.10.003

Cited by 4 publications

(2 citation statements)

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“…The cell‐free in vitro transcription/translation systems can be used to measure the activity of ribosomal antibiotics with an inhibitory value readout. However, most of these methods require the preparation of high‐purity and active bacterial ribosomes, which involve cumbersome steps such as sucrose gradient centrifugation, 137,138 and the whole process is slow and expensive. For cell‐based in vitro translation inhibition systems, it does not require pure ribosomes.…”

Section: Challenges and Advances In Ribosomal Antibiotics And Bacterial Ribosomesmentioning

confidence: 99%

“…For a long time, cell‐free in vitro transcription/translation screening systems have been widely used for the rapid discovery of molecules that specifically target ribosomes 131 . However, most of these systems require purified bacteria ribosomes, of which the purification is quite cumbersome due to the complexity of ribosomes 137,138 . Therefore, we have developed an integrated synthesis/activity test platform, which employs the crude ribosome extract for target‐oriented synthesis and activity test and is time‐saving and less equipment‐dependent 139 .…”

Section: Opportunities and Perspectivementioning

confidence: 99%

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Ribosome‐targeting antibacterial agents: Advances, challenges, and opportunities

Zhang

Bai

et al. 2021

Medicinal Research Reviews

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Ribosomes, which synthesize proteins, are critical organelles for the survival and growth of bacteria. About 60% of approved antibiotics discovered so far combat pathogenic bacteria by targeting ribosomes. However, several issues, such as drug resistance and toxicity, have impeded the clinical use of ribosome‐targeting antibiotics. Moreover, the complexity of the bacteria ribosome structure has retarded the discovery of new ribosome‐targeting agents that are considered as the key to the drug‐resistance and toxicity. To deal with these challenges, efforts such as medicinal chemistry optimization, combination treatment, and new drug delivery system have been developed. But not enough, the development of structural biology and new screening methods bring powerful tools, such as cryo‐electron microscopy technology, advanced computer‐aided drug design, and cell‐free in vitro transcription/translation systems, for the discovery of novel ribosome‐targeting antibiotics. Thus, in this paper, we overview the research on different aspects of bacterial ribosomes, especially focus on discussing the challenges in the discovery of ribosome‐targeting antibacterial drugs and advances made to address issues such as drug‐resistance and selectivity, which, we believe, provide perspectives for the discovery of novel antibiotics.

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Section: Challenges and Advances In Ribosomal Antibiotics And Bacterial Ribosomesmentioning

confidence: 99%