Kinetic of orange pigment production from Monascus ruber on submerged fermentation

Abstract

Pigments produced by species of Monascus have been used to coloring rice, meat, sauces, wines and beers in East Asian countries. Monascus can produce orange (precursor), yellow and red pigments. Orange pigments have low solubility in culture media and when react with amino groups they become red and largely soluble. The orange pigments are an alternative to industrial pigment production because the low solubility facilitates the downstream operations. The aim of this work was to study the kinetic on the production of orange pigments by Monascus ruber CCT 3802. The shaking frequency of 300 rpm was favorable to production, whereas higher shaking frequencies showed negative effect. Pigment production was partially associated with cell growth, the critical dissolved oxygen concentration was between 0.894 and 1.388 mgO L at 30 °C, and limiting conditions of dissolved oxygen decreased the production of orange pigments. The maintenance coefficient (mo) and the conversion factor of oxygen in biomass (Yo) were 18.603 mgO g h and 3.133 g gO and the consideration of these parameters in the oxygen balance to estimate the biomass concentration provided good fits to the experimental data.

Changing oxidoreduction potential to improve water-soluble yellow pigment production with Monascus ruber CGMCC 10910

“…The results showed that the yield of yellow pigments (both extracellular and intracellular) and cell growth under oxidative conditions were significantly increased (Fig. 3 b–d), and it has been reported that Monascus pigment production was coupled with cell growth [ 47 ]; thus, the HIO condition increased yellow pigment production primarily by enhancing cell growth. In contrast, the reducing environment inhibited the biosynthesis of yellow pigments (Fig.…”

Production and Characterization of Anti-Inflammatory Monascus Pigment Derivatives

“…This result implies that oxygen is necessary not only for cell growth but also for orange pigment production. Indeed, the production of orange pigments was reported to be reduced by the limitation of dissolved oxygen [ 41 ], and an improvement in oxygen supply led to an increase in Monascus pigments [ 42 , 43 , 44 ]. In addition, oxygen is a substrate of a flavin adenine dinucleotide-dependent monooxygenase, which is involved in a biosynthetic pathway for orange pigments [ 29 , 45 , 46 ].…”

Estudo comparativo entre espécies de Monascus para produção de pigmentos naturais utilizando resíduo agroindustrial como substrato

“…Ao final do cultivo, o meio fermentado foi filtrado sob vácuo utilizando papel filtro quantitativo. O sobrenadante foi centrifugado a 4.500 x g por 20 min a 23 °C e, em seguida, a produção dos pigmentos extracelulares vermelhos foi expressa em Unidade de Absorbância (UA) utilizando espectrofotômetro (Libra S6, Biochrom®) no comprimento de onda de 510 nm (vermelho) (Vendruscolo et al, 2017).…”

“…Os corantes naturais além de conferirem cor, apresentam princípios benéficos à saúde dos consumidores, como a capacidade antioxidante, que promove a melhora na circulação sanguínea e dos batimentos cardíacos. Por essa razão, o emprego de corantes naturais vem se tornando uma alternativa eficiente e interessante na indústria de alimentos, visando oferecer ao consumidor produtos saudáveis e seguros (Rocha & Reed, 2014;Lermen et al, 2015;Vendruscolo et al, 2017).…”

“…A literatura reporta mais de 20 espécies de Monascus, sendo Mosnacus purpureus e Monascus ruber as mais utilizadas para a síntese de pigmentos, capazes de metabolizar celulose, maltose, frutose e glicose. Destaca-se que diferentes espécies deste gênero podem apresentar comportamentos distintos em relação à produção de pigmentos em um mesmo meio de produção (Wang et al, 2016;Jin & Pyo, 2017;Vendruscolo et al, 2017;Yuliana et al, 2017;Zhang et al, 2018;Sánchez-Muñoz et al, 2020).…”