Comprehensive microbiome analysis of tonsillar crypts in IgA nephropathy

Watanabe, Hirofumi; Goto, Shin; Mori, Hiroshi; Higashi, Koichi; Hosomichi, Kazuyoshi; Aizawa, Naotaka; Takahashi, Nao; Tsuchida, M; Suzuki, Yusuke; Yamada, Takuji; Horii, Arata; Inoue, Ituro; Kurokawa, Ken; Narita, Ichiei

doi:10.1093/ndt/gfw343

Cited by 32 publications

(33 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, IgAN patients presenting with proteinuria levels ≥ 1 g/24 h were substantially more likely to progress to end-stage renal disease, indicating that intestinal ora imbalance, characterized by an increased abundance of pathogenic bacteria and reduction in that of bene cial strains, may be relevant to IgAN progression [18,19]. Recently, the substantial diversity in bacterial composition from tonsillar crypts of IgA patients was detected by Watanabe H [20]. In their study, abundance of Prevotella spp., Haemophilus spp., Porphyromonas spp.…”

Section: Discussionmentioning

confidence: 97%

Modifications of abundances of some bacteria are associated with the severity of IgA nephropathy in Chinese population

Zhong

Tan

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background Immunoglobulin A nephropathy (IgAN) is a common autoimmune glomerular disease, the classic manifestations of which are hematuria and proteinuria. The pathogenesis of IgAN has been associated with dysregulated intestinal mucosal immunity. However, whether gut microbial modifications play a part in IgAN remains unclear. Methods Blood and fecal samples were collected from 52 IgAN patients and 25 healthy controls (HCs). The gut microbiome was analyzed by 16S ribosomal RNA (rRNA) gene sequencing, followed by analyses of gut microbiota composition. Furthermore, the levels of galactose-deficient IgA1 (Gd-IgA1), soluble cluster of differentiation 14 (sCD14), lipopolysaccharide binding protein (LBP), intercellular adhesion molecule-1 (ICAM-1), and C-reactive protein (CRP) was quantified by enzyme-linked immunosorbent assay (ELISA). Results Substantial differences in gut microbiota were found between IgAN patients and HCs (P < 0.05). The abundance of pathogenic bacteria (Bacteroides and Escherichia-Shigella) was significantly higher in IgAN patients than in HCs, while that of beneficial strains (Bifidobacterium and Blautia spp.) was lower. Higher proportions of Escherichia-Shigella and lower proportions of Bifidobacterium spp. were observed in IgAN patients with a high urine RBC count (≥ 10/HP) and proteinuria (≥ 1 g/24 h) levers. Spearman’s correlation analysis was used to assess the association between gut microbiota and biomarkers in IgAN patients. The results showed that the genus Prevotella 7 was negatively correlated with Gd-IgA1, LBP, sCD14, and ICAM-1, while Bifidobacterium spp. presented a significant inverse relationship with LBP and Gd-IgA1. Additionally, Escherichia-Shigella was negatively correlated with Prevotella 7. Conclusions In IgAN patients, gut modifications were characterized by an increase in the numbers of pathogenic bacteria and a reduction in the levers of beneficial bacteria. Our results suggest that disturbance of the intestinal microflora might play a critical part in the severity of IgAN, and may be a potential therapeutic target.

show abstract

Section: Discussionmentioning

confidence: 97%

Modifications of abundances of some bacteria are associated with the severity of IgA nephropathy in Chinese population

Zhong

Tan

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The microbiota signature in the tonsils of patients with IgAN has not been fully elucidated. A recent study of the tonsillar microbiota did not show any difference among IgAN, recurrent tonsillitis, and tonsillar hypertrophy 23 . However, this previous study was based on single-centre data, and urinalysis data were lacking for paediatric patients in the control group, making it impossible to exclude hidden glomerular diseases.…”

mentioning

confidence: 77%

Comparative analysis of the tonsillar microbiota in IgA nephropathy and other glomerular diseases

Park

Kim

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Immunoglobulin A nephropathy (IgAN) involves repeated events of gross haematuria with concurrent upper airway infections. The mucosal immune system, especially the tonsil, is considered the initial site of inflammation, although the role of the tonsillar microbiota has not been established in IgAN. In this study, we compared the tonsillar microbiota of patients with IgAN (n = 21) and other glomerular diseases (n = 36) as well as, healthy controls (n = 23) from three medical centres in Korea. The microbiota was analysed from tonsil swabs using the Illumina MiSeq system based on 16S rRNA gene. Tonsillar bacterial diversity was higher in IgAN than in other glomerular diseases, although it did not differ from that of healthy controls. Principal coordinates analysis revealed differences between the tonsillar microbiota of IgAN and both healthy and disease controls. The proportions of Rahnella, Ruminococcus_g2, and Clostridium_g21 were significantly higher in patients with IgAN than in healthy controls (corrected p < 0.05). The relative abundances of several taxa were correlated with the estimated glomerular filtration rate, blood urea nitrogen, haemoglobin, and serum albumin levels. Based on our findings, tonsillar microbiota may be associated with clinical features and possible immunologic pathogenesis of IgAN.

show abstract

“…After merging the forward and reverse reads, we obtained high-quality reads, and operational taxonomic unit (OTU) clustering and taxonomic assignment were performed as previously described [ 11 , 12 ]. In brief, each forward and reverse high-quality read for the paired-end library was merged using an USEARCH (version 8.0.1517).…”

Section: Methodsmentioning

confidence: 99%

Cecal Microbiome Analyses on Wild Japanese Rock Ptarmigans (Lagopus muta japonica) Reveals High Level of Coexistence of Lactic Acid Bacteria and Lactate-Utilizing Bacteria

et al. 2018

Self Cite

View full text Add to dashboard Cite

Preservation of indigenous gastrointestinal microbiota is critical for successful captive breeding of endangered wild animals, yet its biology is poorly understood. Here, we compared the cecal microbial composition of wild living Japanese rock ptarmigans (Lagopus muta japonica) in different locations of Japanese mountains, and the dominant cecal microbial structure of wild Japanese rock ptarmigans is elucidated. Coriobacteraceae and Lachnospraceae were the two dominant bacterial families in all samples analyzed. At the genus level, 10 genera Olsenella, Actinomyces, Megasphaera, Slackia, Cloacibacillus, Bifidobacterium, Escherichia, Dialister, Megamonas, and Bilophila were dominant. These results reveal the high level of coexistence of lactic acid bacteria (Olsenella and Bifidobacterium) and lactate-utilizing bacteria (Megasphaera). This coexistence should be taken into account for the successful breeding of captive Japanese rock ptarmigans in the national conservation program.

show abstract

Comprehensive microbiome analysis of tonsillar crypts in IgA nephropathy

Abstract: Similar patterns of bacteria are present in tonsillar crypts of both IgAN and RT patients, suggesting that the host response to these bacteria might be important in the development of IgAN.

Cited by 32 publications

References 44 publications

Modifications of abundances of some bacteria are associated with the severity of IgA nephropathy in Chinese population

Modifications of abundances of some bacteria are associated with the severity of IgA nephropathy in Chinese population

Comparative analysis of the tonsillar microbiota in IgA nephropathy and other glomerular diseases

Cecal Microbiome Analyses on Wild Japanese Rock Ptarmigans (Lagopus muta japonica) Reveals High Level of Coexistence of Lactic Acid Bacteria and Lactate-Utilizing Bacteria

Contact Info

Product

Resources

About