Evaluation of Genotoxic Pressure along the Sava River

Abstract

In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg—modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg—modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).

Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia

“…6 PCA graph and grouping of analysed fish specimens using as input variables: genotoxicity parameters (TI% in blood, liver and gills), element concentration (B, Ca, Cr, Cu, Fe, K, Mg, Mn, Mo, Se, Sr, Zn) in muscle (M) and liver (L), histopathological index of gills (IG) and liver (IL) and abundance of immature, intermediary and mature erythrocytes high frequencies of MN only in top predator fish. Our findings are consistent with previous research that showed higher sensitivity of comet assay in comparison to MN to estimate the genotoxic potential and discriminate fine differences in the response of ecologically similar species (De Andrade et al 2004;Grisolia et al 2009;Kolarević et al 2016).…”

An integrated approach for chemical water quality assessment of an urban river stretch through Effect-Based Methods and emerging pollutants analysis with a focus on genotoxicity

“…Overall, the results obtained with genotoxicity assays showed that the Comet assay can be a useful tool in biomonitoring of surface waters applying several experimental models in agreement with literature data (Guidi et al, 2017;Kolarevic et al, 2016a;Kolarević et al, 2016b;Deutschmann et al, 2016;Pellegri et al, 2020).…”

Investigação de Alterações Genotóxicas em Hoplosternum littoralle, Frente a Contaminação Ambiental, com o Uso do Teste do Micronúcleo

“…Nas últimas décadas, a industrialização e as diversas influências antropogênicas resultaram em uma maior presença de contaminantes em ecossistemas aquáticos (Sunjog et al, 2013). Isso ocorre pois o que é descartado contêm mistura de contaminantes orgânicos e inorgânicos, o que pode acarretar múltiplos efeitos biológicos, induzir alterações genéticas e influenciar a integridade de moléculas de DNA em organismos aquáticos, isso se dá pela interação com múltiplas sequências alvo nos organismos expostos (Kolarevic et al, 2017& Thellmann et al, 2017. Uma das formas de identificar a presença de agentes genotóxicos é o teste do micronúcleo, uma técnica simples que oferece rápido resultado (Bueno et al, 2017).…”