Proteomic analysis of the vitamin C effect on the doxorubicin cytotoxicity in the MCF-7 breast cancer cell line

Bober, Peter; Alexovič, Michal; Talian, Ivan; Tomkova, Zuzana; Viscorova, Zuzana; Benckova, Maria; Andrašina, Igor; Ciccocioppo, Rachele; Petrovič, Daniel; Adamek, Mariusz; Kružliak, Peter; Sabó, Ján

doi:10.1007/s00432-016-2259-4

Cited by 21 publications

(14 citation statements)

References 38 publications

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“…RPL27A), transcriptional (eg. HNRNPH1), immune system and antioxidant (HSP90AA1, SOD1) proteins in DOX + VitC-treated cells - Upregulation of GAPDH, GPI and ACTA1 [ 167 ] Leukemia HL-60 cell line LC-MS/MS 48 h treatment with 10 μM As2O3 (arsenic trioxide) or As2O3 + 100 μM L-AA (ascorbic acid) + 50 μM α-TOC (α-tocopherol) Chemo + Dietary suppl. low Evaluate the synergistic mechanism of action of vitamins, such as L-ascorbic acid (L-AA) and a-tocopherol (a-TOC) in As2O3 chemotherapy - Number of identified proteins n.s.…”

Section: Global Molecular Profiling Studies On High-dose Ivc In the Cancer Contextmentioning

confidence: 99%

High-dose intravenous vitamin C, a promising multi-targeting agent in the treatment of cancer

Böttger

Vallés-Martí

Cahn

et al. 2021

J Exp Clin Cancer Res

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Mounting evidence indicates that vitamin C has the potential to be a potent anti-cancer agent when administered intravenously and in high doses (high-dose IVC). Early phase clinical trials have confirmed safety and indicated efficacy of IVC in eradicating tumour cells of various cancer types. In recent years, the multi-targeting effects of vitamin C were unravelled, demonstrating a role as cancer-specific, pro-oxidative cytotoxic agent, anti-cancer epigenetic regulator and immune modulator, reversing epithelial-to-mesenchymal transition, inhibiting hypoxia and oncogenic kinase signalling and boosting immune response. Moreover, high-dose IVC is powerful as an adjuvant treatment for cancer, acting synergistically with many standard (chemo-) therapies, as well as a method for mitigating the toxic side-effects of chemotherapy. Despite the rationale and ample evidence, strong clinical data and phase III studies are lacking. Therefore, there is a need for more extensive awareness of the use of this highly promising, non-toxic cancer treatment in the clinical setting. In this review, we provide an elaborate overview of pre-clinical and clinical studies using high-dose IVC as anti-cancer agent, as well as a detailed evaluation of the main known molecular mechanisms involved. A special focus is put on global molecular profiling studies in this respect. In addition, an outlook on future implications of high-dose vitamin C in cancer treatment is presented and recommendations for further research are discussed.

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Section: Global Molecular Profiling Studies On High-dose Ivc In the Cancer Contextmentioning

confidence: 99%

High-dose intravenous vitamin C, a promising multi-targeting agent in the treatment of cancer

Böttger

Vallés-Martí

Cahn

et al. 2021

J Exp Clin Cancer Res

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“…For example, while supplementation with carotenoids may increase mortality in breast cancer patients 170 , supplementation with vitamin C and E in the same patients was associated with reduced recurrence rate 170,171 . Vitamin C also potentiates the anti-proliferative effect of doxorubicin in breast cancer 172 while high vitamin D levels are associated with increased survival in colorectal cancer patients 173 .…”

Section: Targeting Mros and Anti-oxidant Systems In Anticancer Therapymentioning

confidence: 99%

Mitochondrial ROS control of cancer

Idelchik

Begley

et al. 2017

Seminars in Cancer Biology

248

169

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Mitochondria serve a primary role in energy maintenance but also function to govern levels of mitochondria-derived reactive oxygen species (mROS). ROS have long been established to play a critical role in tumorigenesis and are now considered to be integral to the regulation of diverse signaling networks that drive proliferation, tumor cell survival and malignant progression. mROS can damage DNA, activate oncogenes, block the function of tumor suppressors and drive migratory signaling. The mitochondrion's oxidant scavenging systems including SOD2, Grx2, GPrx, Trx and TrxR are key of the cellular redox tone. These mitochondrial antioxidant systems serve to tightly control the levels of the primary ROS signaling species, H2O2. The coordinated control of mROS levels is also coupled to the activity of the primary H2O2 consuming enzymes of the mitochondria which are reliant on the epitranscriptomic control of selenocysteine incorporation. This review highlights the interplay between these many oncogenic signaling networks, mROS and the H2O2 emitting and consuming capacity of the mitochondria.

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“…Another important hypothesis is that vitamin C can create important epigenetic changes due to its effect on 2-oxoglutarate-dependent dioxygenases, like histone and DNA demethylases [6]. In preclinical studies investigators also show that vitamin C can have a synergetic effect with some types of chemo- and immunotherapy [7,8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Vitamin C (Ascorbic Acid) in the Treatment of Patients with Cancer: A Systematic Review

et al. 2019

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Many cancer patients on intensive chemotherapy lack vitamin C. Vitamin C stimulates the production and activation of immune cells, so perhaps supplementation could be used to improve the immunity in those patients. This review assesses the effectiveness and safety of vitamin C administration in cancer. The PubMed and EMBASE databases were searched and all study designs except for phase I studies, and case reports were included in this review. A total of 19 trials were included. In only 4 trials randomization was used to determine if patients received vitamin C or a placebo. The result of this review does not prove that there is a clinically relevant positive effect of vitamin C supplementation in cancer patients in general on the overall survival, clinical status, quality of life (QOL) and performance status (PS), since the quality of the studies published is low. Interventions and patient groups are very diverse, hence an effect in some patient groups is possible. There seems to be a better effect with intravenous than oral administration. Nevertheless, treatment with vitamin C is safe with minimal side effects. Thereby, we think it is safe to examine the effects of vitamin C on specific groups of patients in a randomized controlled setting.

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Proteomic analysis of the vitamin C effect on the doxorubicin cytotoxicity in the MCF-7 breast cancer cell line

Abstract: The detected proteins may be capable of predicting response to DOX therapy. This is a key tool in the treatment of breast cancer, and the combination with vit C seems to be of particular interest due to the fact that it can potentiate anti-proliferative effect of DOX.

Cited by 21 publications

References 38 publications

High-dose intravenous vitamin C, a promising multi-targeting agent in the treatment of cancer

High-dose intravenous vitamin C, a promising multi-targeting agent in the treatment of cancer

Mitochondrial ROS control of cancer

The Effect of Vitamin C (Ascorbic Acid) in the Treatment of Patients with Cancer: A Systematic Review

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