Effect of polyamines on mechanical and structural properties of <i>Bombyx mori</i> silk

Yerra, Aparna; Mysarla, Danti Kumari; Siripurapu, Prasanthi; Jha, Anjali; Valluri, Satyavathi V; Mamillapalli, Anitha

doi:10.1002/bip.22980

Cited by 14 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spd is a biologically important polyamine that serves as a key regulator of processes like DNA stability, protein synthesis, cell proliferation, differentiation, and apoptosis [17][18][19]. The potential effect of Spd supplementation to B. mori efficiently enhanced the larval weights, silk gland weights, silk quality and quantity, and mechanical and structural properties of the silk fibres [20,21].…”

Section: Objectivementioning

confidence: 99%

16S rRNA sequence data of Bombyx mori gut bacteriome after spermidine supplementation

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objectives: The silkworm Bombyx mori (B. mori) is an important domesticated lepidopteran model for basic and applied research. They produce silk fibres that have great economic value. The gut microbiome plays an important role in the growth of organisms. Spermidine (Spd) is shown to be important for the growth of all living cells. The effect of spermidine feeding on the gut microbiome of 5th instar B. mori larvae was checked. The B. mori gut samples from control and spermidine fed larvae were subjected to next-generation sequencing analysis to unravel changes in the bacterial community upon spermidine supplementation. Data description: The changes in gut bacteriota after spermidine feeding is not studied before. B. mori larvae were divided into two groups of 50 worms each and were fed with normal mulberry leaves and mulberry leaves fortified with 50 µM spermidine. The gut tissues were isolated aseptically and total genomic DNA was extracted, 16S rRNA region amplified and sequenced using Illumina platform. The spermidine fed gut samples were shown to have abundance and diversity of the phyla Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria.

show abstract

Section: Objectivementioning

confidence: 99%

16S rRNA sequence data of Bombyx mori gut bacteriome after spermidine supplementation

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Silk cocoons spun by silkworm larvae are composed of two major proteins, fibroin and sericin [6], and are widely used in textiles, medical engineering, and industrial applications [7][8][9][10][11]. In the Compendium of Materia Medica, silk cocoons are reported to cure carbuncles.…”

Section: Introductionmentioning

confidence: 99%

Silk fibroin peptide suppresses proliferation and induces apoptosis and cell cycle arrest in human lung cancer cells

Wang

Huang

et al. 2018

Acta Pharmacol Sin

View full text Add to dashboard Cite

Silkworm cocoon was recorded to cure carbuncle in the Compendium of Materia Medica. Previous studies have demonstrated that the supplemental silk protein sericin exhibits anticancer activity. In the present study, we investigated the effects of silk fibroin peptide (SFP) extracted from silkworm cocoons against human lung cancer cells in vitro and in vivo and its possible anticancer mechanisms. SFP that we prepared had high content of glycine (~ 30%) and showed a molecular weight of ~ 10 kDa. Intragastric administration of SFP (30 g/kg/d) for 14 days did not affect the weights, vital signs, routine blood indices, and blood biochemical parameters in mice. MTT assay showed that SFP dose-dependently inhibited the growth of human lung cancer A549 and H460 cells in vitro with IC values of 9.921 and 9.083 mg/mL, respectively. SFP also dose-dependently suppressed the clonogenic activity of the two cell lines. In lung cancer H460 xenograft mice, intraperitoneal injection of SFP (200 or 500 mg/kg/d) for 40 days significantly suppressed the tumor growth, but did not induce significant changes in the body weight. We further examined the effects of SFP on cell cycle and apoptosis in H460 cells using flow cytometry, which revealed that SFP-induced cell cycle arrest at the S phase, and then promoted cell apoptosis. We demonstrated that SFP (20-50 mg/mL) dose-dependently downregulates Bcl-2 protein expression and upregulates Bax protein in H460 cells during cell apoptosis. The results suggest that SFP should be studied further as a novel therapeutic agent for the treatment of lung cancer.

show abstract

“…Their levels are strictly controlled by complex metabolic pathways incorporating polyamine biosynthesis, catabolism, and transport [ 3 , 4 ]. They are essential for the survival and growth of eukaryotic cells by regulating gene expression and protein synthesis, affecting a large variety of cellular processes, including cell growth, differentiation, and regulation [ 5 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

A spermidine-based nutritional supplement prolongs the anagen phase of hair follicles in humans: a randomized, placebo-controlled, double-blind study

Rinaldi

Mora

Pinto

et al. 2017

Dermatol Pract Concept

View full text Add to dashboard Cite

BackgroundSpermidine has been shown both in vitro and in mice models to have an anagen-prolonging effect on hair follicles (HFs).ObjectivesTo evaluate the effects of a spermidine-based nutritional supplement on the anagen phase of HFs in healthy human subjects in a randomized, double-blind, placebo-controlled trial.MethodsOne hundred healthy males and females were randomized to receive a tablet containing a spermidine-based nutritional supplement or a placebo once daily for 90 days. At the beginning and the end of the treatment period, 100 HFs were plucked and subjected to microscopic evaluation to determine the number of anagen V–VI HFs, and immunohistochemical examination was performed to quantify the Ki-67 and c-Kit levels in the hair bulbs. Pull test was performed after three and six months.ResultsThe spermidine-based nutritional supplement increased the number of anagen V–VI HFs after three months of treatment, accompanied by increased Ki-67, a marker for cellular proliferation, and decreased c-Kit, a marker for apoptosis, levels. All results were also significantly better when compared to the placebo group. The pull test remained negative after six months in all patients receiving the spermidine supplement, while 68% of the subjects in the placebo group had a positive pull test.ConclusionsThis preliminary study shows that a spermidine-based nutritional supplement can prolong the anagen phase in humans, and therefore might be beneficial for hair loss conditions. Further studies are needed to evaluate its effects in specific different clinical settings.

show abstract

Effect of polyamines on mechanical and structural properties of Bombyx mori silk

Cited by 14 publications

References 24 publications

16S rRNA sequence data of Bombyx mori gut bacteriome after spermidine supplementation

16S rRNA sequence data of Bombyx mori gut bacteriome after spermidine supplementation

Silk fibroin peptide suppresses proliferation and induces apoptosis and cell cycle arrest in human lung cancer cells

A spermidine-based nutritional supplement prolongs the anagen phase of hair follicles in humans: a randomized, placebo-controlled, double-blind study

Contact Info

Product

Resources

About