Serum biomarkers for allergy in children

Knipping, Karen; Knippels, L.M.J.; Dupont, Christophe; Garssen, Johan

doi:10.1111/pai.12649

Cited by 17 publications

(18 citation statements)

References 106 publications

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“…Cytokine levels and other mediators have been investigated in patients with rhinitis using the nasal fluid [8, 9, 18], either to get more insight regarding the pathogenic condition, for diagnostic purposes, or to measure therapeutic effects. However, no single or specific biomarker for AR has been identified to date, especially in children’s serum, for predicting the severity of the condition [9, 18, 19]. In children, measurements of inflammatory markers are inconsistent across the different (sampling) techniques reflecting disease heterogeneity, methodological limitations, or varying sensitivity of the biomarker detection techniques [9, 12, 18, 19].…”

Section: Discussionmentioning

confidence: 99%

“…Especially in (young) children, reliable, non-invasive biomarkers would be valuable. Cytokine levels and other mediators have been investigated in patients with rhinitis using the nasal fluid [8, 9, 18], either to get more insight regarding the pathogenic condition, for diagnostic purposes, or to measure therapeutic effects. However, no single or specific biomarker for AR has been identified to date, especially in children’s serum, for predicting the severity of the condition [9, 18, 19].…”

Section: Introductionmentioning

confidence: 99%

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Serum IL-1β can be a biomarker in children with severe persistent allergic rhinitis

Han

Kim

et al. 2019

Allergy Asthma Clin Immunol

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Background Allergic rhinitis (AR) is one of the most common diseases globally and usually persists throughout life. In the present study, we aimed to determine whether the expression of inflammatory biomarkers has a relationship with the severity of allergic rhinitis and with comorbid asthma or other allergic diseases in children. Methods For diagnosis of AR, the skin prick test was performed to measure the responses to 18 allergens. Blood levels of eosinophils and immunoglobulin E (IgE) were examined. We classified the patients into 2 groups based on the severity of the condition as Group 1 [intermittent AR (IAR) or mild persistent AR (PAR)] and Group 2 (moderate to severe PAR). To determine the expression of inflammatory biomarkers, in serum and several biomarkers (caspase-1, IL-1β, CCL-11, CCL-24 and IL-33) were measured in the serum using enzyme-linked immunosorbent assay (ELISA). Additionally, we analyzed the correlation between clinical variables and the expression of biomarkers (eosinophils count, IL-1β and CCL-24) and the severity of AR. Results We found that eosinophils count, IL-1β, a marker of activation of inflammasomes, and CCL-24 were significantly increased in the moderate to severe PAR group (p = 0.008, p = 0.003, p = 0.039). Additionally, the expressions of eosinophil count, IL-1β and CCL-24 were significantly higher in patients with active asthmatic symptoms than in those without these conditions. On univariate analysis, allergic rhinitis in sibling, paternal allergic rhinitis, high expression of eosinophils count, IL-1β and CCL-24, history of active asthma and atopy correlated with severity of AR. Multivariate analysis showed only paternal allergic rhinitis and high expression of IL-1β as significant risk factors of moderate to severe PAR with 6.4 fold and 4.7 fold-increase in risk, respectively (p = 0.011 and p = 0.030). Conclusion In conclusion, this study provides the first evidence that an excessive release of biologically active IL-1β may promote inflammation in severe PAR. It demonstrates that IL-1β can be a biomarker for active allergic diseases such as AR, asthma, and atopy. Moreover, this finding suggests that IL-1B should be investigated as a therapeutic target in severe PAR and other allergic diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serum IL-1β can be a biomarker in children with severe persistent allergic rhinitis

Han

Kim

et al. 2019

Allergy Asthma Clin Immunol

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“…Since elevated serum levels of IgE and IL-4 are considered as important biological markers of allergic diseases such as AD and athma, we also evaluated whether the sample influences the blood IgE and IL-4 concentrations of AD mice. 27) Treatment of JCE markedly diminished total IgE and IL-4 levels in the serum of DNCB-induced atopic mice. The results indicated that both topical and broad applications of JCE could improve atopic damage in murine AD models.…”

Section: Discussionmentioning

confidence: 93%

“…23,25) A powerful example of this is in the case of pimecrolimus (Elidel ® ), one representative component available for the treatment of AD, derived from ascomycin which is a natural microorganism from soil actinomycetes. 26) For this reason, plant resources including extracts, pure compounds, and phytochemical combinations have attracted continuous interest to find drug candidates for the prevention and/or treatment of atopic diseases.…”

Section: Discussionmentioning

confidence: 99%

<i>Juniperus chinensis</i> Fruits Attenuate Oxazolone- and 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis Symptoms in Mice

Jegal

Park

et al. 2018

Biological & Pharmaceutical Bulletin

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Juniperus chinensis, commonly Chinese juniper, has been used for treating inflammatory diseases. This study aimed to investigate anti-atopic dermatitis (AD) effects of standardized J. chinensis fruits extract on murine oxazolone- and 2,4-dinitrochlorobenzene (DNCB)-induced models of AD. Ear swelling, epidermis thickening, and eosinophils infiltration in the oxazolone-mediated dermatitis of BALB/c mice were significantly reduced upon topical application of J. chinensis fruits 95% EtOH extract (JCE). Besides, transdermal administration of JCE to SKH-1 hairless mice inhibited the development of DNCB-induced AD-like skin lesions by suppressing transepidermal water loss and improving skin hydration. Decreased total serum immunoglobulin E (IgE) and interleukin (IL)-4 levels could be observed in atopic dorsal skin samples of JCE-treated group. According to the phytochemical analysis, JCE was found to contain isoscutellarein-7-O-β-D-xyloside, cupressuflavone, and amentoflavone as main compounds. Therapeutic attempts with the J. chinensis fruits might be useful in the treatment of AD and related skin inflammatory diseases.

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“…However, the relation between sIgE serum levels and adversity of the allergic responses varies, thus sIgE can occur in subjects without clinical food allergy symptoms and vice versa [ 10 – 12 ]. Also, other serum markers, such as IgG4 or cytokines, have been evaluated but were not validated as reliable diagnostic markers [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food

et al. 2020

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Background: The underlying cellular mechanisms causing adverse reactions to food are complex and still not fully understood. Therefore, in this study we aimed to identify functional and/or phenotypical immune cell signatures characteristic for adult patients reporting adverse reactions to food. By mass cytometry, we performed high-dimensional profiling of peripheral blood mononuclear cells (PBMC) from adult patients reporting adverse reactions to food and healthy controls. The patients were grouped according to sIgE-positive or sIgE-negative serology to common food and inhalant allergens. Two broad antibody panels were used, allowing determination of major immune cell populations in PBMC, as well as activation status, proliferation status, and cytokine expression patterns after PMA/ionomycin-stimulation on a single cell level. Results: By use of data-driven algorithms, several cell populations were identified showing significantly different marker expression between the groups. Most striking was an impaired frequency and function of polyfunctional CD4+ and CD8+ T cells in patients reporting adverse reactions to food compared to the controls. Further, subpopulations of monocytes, T cells, and B cells had increased expression of functional markers such as CD371, CD69, CD25, CD28, and/or HLA-DR as well as decreased expression of CD23 in the patients. Most of the differing cell subpopulations were similarly altered in the two subgroups of patients. Conclusion: Our results suggest common immune cell features for both patient subgroups reporting adverse reactions to food, and provide a basis for further studies on mechanistic and diagnostic biomarker studies in food allergy.

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Serum biomarkers for allergy in children

Cited by 17 publications

References 106 publications

Serum IL-1β can be a biomarker in children with severe persistent allergic rhinitis

Serum IL-1β can be a biomarker in children with severe persistent allergic rhinitis

<i>Juniperus chinensis</i> Fruits Attenuate Oxazolone- and 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis Symptoms in Mice

Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food

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