Genotyping of Brucella melitensis and Brucella abortus strains currently circulating in Xinjiang, China

Sun, Mingjun; Di, Dongdong; Li, Yan; Zhang, Zhicheng; Yan, Huan; Tian, Lili; Jing, Zhaoyang; Li, Jinping; Jiang, Hai; Fan, Weixing

doi:10.1016/j.meegid.2016.07.025

Cited by 25 publications

(20 citation statements)

References 12 publications

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“…This result is consistent with previous reports (Jiang et al, 2011; Xiao et al, 2015). With regard to MLVA-8 genotypes, the most frequently observed genotype in this study, genotype 42, was also observed in other regions of China (Jiang et al, 2011; Ma et al, 2016; Sun et al, 2016). MLVA-8 and MLVA-11 yielded three and eight genotypes, respectively; whereas the addition of panel 2B increased the number of genotypes to 69.…”

Section: Discussionsupporting

confidence: 82%

MLVA Genotyping Characteristics of Human Brucella melitensis Isolated from Ulanqab of Inner Mongolia, China

Liu

Wang

et al. 2017

Front. Microbiol.

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Brucellosis is a serious public health problem in Ulanqab, which is a region located in the middle of the Inner Mongolia Autonomous Region adjacent to Shanxi and Hebei provinces. The disease is prevalent in both the latter provinces and Ulanqab with the highest prevalence of brucellosis occurring in Inner Mongolia. The MLVA-16 scheme is a genotyping tool for assessing genetic diversity and relationships among isolates. Moreover, this genotyping tool can also be applied to epidemiological trace-back investigations. This study reports the occurrence of at least two B. melitensis biovars (1 and 3) in Ulanqab, encompassing 22 and 94 isolates, respectively. B. melitensis biovar 3 was the predominant biovar in the area examined. Panel 1 (MLVA-8) identified three genotypes (42, 63, and 114), with genotype 42 (n = 101) representing 87% of the tested strains. MLVA-11 identified eight genotypes (116, 111, 297, 291, and 342–345) from 116 of the analyzed isolates. All of these isolates were identified as belonging to the East Mediterranean group. Genotype 116 (n = 94) was the predominant genotype and represented 81% of the isolates. The isolates pertaining to this genotype were distributed throughout most of Ulanqab and neighboring regions. The MLVA-16 scheme showed the presence of 69 genotypes, with 46 genotypes being represented by single isolates. This analysis revealed that Ulanqab brucellosis cases had epidemiologically unrelated and sporadic characteristics. The remaining 23 genotypes were shared (between a total of 70 isolates) with each genotype being represented by two to eight isolates. These data indicate that these cases were epidemiologically related. MLVA genotyping confirmed the occurrence of a multipoint outbreak epidemic and intrafamilial brucellosis. Extensive genotype-sharing events were observed among isolates from different regions of Ulanqab and from other provinces of China. These findings suggest either a lack of control of animal movement between different regions or the circulation of contaminated animal products in the market. Our study is the first comprehensive genotyping and genetic analysis of B. melitensis in Ulanqab. We believe that this study will help to improve the effectiveness of brucellosis control programs.

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Section: Discussionsupporting

confidence: 82%

MLVA Genotyping Characteristics of Human Brucella melitensis Isolated from Ulanqab of Inner Mongolia, China

Liu

Wang

et al. 2017

Front. Microbiol.

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“…3 (n = 23) and B. suis bv. 3 (n = 12) were dominant species, and these results were different from studies from other major areas of China (Ma et al, 2016;Sun et al, 2016). Previous reports show that B. melitensis were the most common in the northwest and northeast China, but B. suis were often observed in the southern regions (Liu et al, 2018;Piao et al, 2018).…”

Section: Discussioncontrasting

confidence: 66%

Molecular Characteristics of Brucella Isolates Collected From Humans in Hainan Province, China

Wang

Zhu

et al. 2020

Front. Microbiol.

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Brucellosis has been reported in several regions of Hainan Province, but the extent of the disease has not been fully elucidated. Conventional biotyping methods, multiple locus variable number tandem repeats analysis (MLVA), and single-nucleotide polymorphisms (SNPs) from draft genome sequencing were employed to characterize the strains. There were four biovars (Brucella melitensis bv. 1, 2, and 3 and Brucella suis bv. 3) detected, which showed that the biovar diversity of Brucella in Hainan is higher than in other areas of China. Both B. melitensis bv. 3 and B. suis bv. 3 were dominant species and showed epidemiology patterns that were compatible with both southern and northern China. Eight of MLVA-11 genotypes were known (31, 111, 116, 120, 136, 291, 297, and 345), and the remaining seven were novel (HN11-1 to HN11-7); these data showed that Brucella strains in this study had multiple geographic origins and exhibited characteristics of origin and evolution of co-existing imported and Hainan specific lineage. A total of 41 strains were found, belonging to 37 unique genotypes that each represented a single strain, which suggests that these strains were not directly related epidemiologically and indicates that the epidemic characteristics of human brucellosis in Hainan was dominated by sporadic strains. The high HGDI values were observed in MLVA-8, MLVA-11, and MLVA-16 among two species, suggesting considerable genetic diversity among these species. MST is characterized based on MLVA-16 that was found both throughout China and on a global level and showed that strains of this study had significant genetic differences with strains from many parts of the globe and seemingly represent a unique genetic lineage. Whole-genome SNP analysis showed that four B. melitensis were closely related to strains from China's northern provinces, and the source of infection was partly of human brucellosis in this province that may have been from these regions. The B. suis were closely related to strains from the United States, and further investigation of the transportation of animals, such as pigs, is needed to elucidate the origins of these strains.

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“…Our hypothesis is sustained by an MST analysis performed on 446 B . melitensis MLVA15 profiles of strains belonging to the East Mediterranean group [37]. In this study, part of the Turkish genotypes clustered with most of the genotypes observed in China and Kazakhstan, suggesting a common origin between strains from the Asian continent and the East Mediterranean area [37].…”

Section: Discussionmentioning

confidence: 74%

Imported human brucellosis in Belgium: Bio and molecular typing of bacterial isolates, 1996-2015

et al. 2017

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ObjectivesThe aim of this study was to characterize by classical biotyping and Multi-Locus variable number tandem repeats (VNTR) Analysis (MLVA) all Brucella spp. derived from human cases in Belgium from 1996 to 2015. Final goals were to determine the species and biovar, to trace-back on genetic grounds the origin of each strain when patient history and risk factors were missing, and to survey for particular trends at the national level.MethodsA total of 37 Brucella strains, isolated from 37 patients in Belgium, were analyzed by both classical biotyping and MLVA, and the genetic patterns compared to those of human strains isolated worldwide.ResultsClassical biotyping revealed that isolates were mainly Brucella melitensis. Most of them belonged to biovar 3, the most abundant biovar in the Mediterranean region. MLVA confirmed that Brucella melitensis is too diverse in VNTRs to be able to make clusters associated to each biovar, but it allowed retrieving precious epidemiological information. The analysis highlighted the imported nature of the strains from all over the world with a dominant part from the Mediterranean countries. Findings of the MLVA11 testing were in line with the travel history of patients coming from Italy, Turkey, Lebanon and Peru. The analysis was particularly useful because it suggested the geographical origin of the infection for 12/16 patients for whom no case history was available.ConclusionClassical biotyping and MLVA analysis are not exclusive but remain complementary tools for Brucella melitensis strain surveillance. MLVA11 is sufficient for Brucella-free countries such as Belgium to trace the geographical origin of infection, but complete MLVA16 is needed to search for links with endemic areas.

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Genotyping of Brucella melitensis and Brucella abortus strains currently circulating in Xinjiang, China

Cited by 25 publications

References 12 publications

MLVA Genotyping Characteristics of Human Brucella melitensis Isolated from Ulanqab of Inner Mongolia, China

MLVA Genotyping Characteristics of Human Brucella melitensis Isolated from Ulanqab of Inner Mongolia, China

Molecular Characteristics of Brucella Isolates Collected From Humans in Hainan Province, China

Imported human brucellosis in Belgium: Bio and molecular typing of bacterial isolates, 1996-2015

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