Spatial-Temporal Study of Rab1b Dynamics and Function at the ER-Golgi Interface

Martinez, Hernán; García, Iris Alejandra; Sampieri, Luciana; Álvarez, Cecilia

doi:10.1371/journal.pone.0160838

Cited by 11 publications

(7 citation statements)

References 51 publications

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“…Rab1A and Rab1B are highly homologous and known to share common biological functions in ER to Golgi trafficking and autophagosome formation [ 26 , 27 ]. Our previous studies have demonstrated that Rab1A is an oncogene whose overexpression is correlated with poor prognosis in patients with CRC and HCC [ 10 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer

Yang

Cui

Zeng

et al. 2017

Aging

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Rab1B has recently been reported to be involved in human cancer, but the role of Rab1B in colorectal cancer (CRC) remains unclear. In this study, we investigated the expression of Rab1B and MMP9 in CRC by qRT-PCR, immunoblot and immunohistochemistry and analyzed the clinical significance. The results show that Rab1B and MMP9 are increased at both mRNA and protein levels in CRC cell lines and tissues, as measured by qRT-PCR and immunoblotting. The high protein expression of Rab1B and MMP9 in 179 CRC tissues is associated with deep tumor invasion, lymph-node metastasis and advanced TNM stage. Survival analysis indicates that patients with overexpression of Rab1B or MMP9 have significantly worse overall survival and progression-free survival, but better response to chemotherapy than those with low expression of proteins, and that Rab1B is an independent prognostic factor for CRC patients. Furthermore, when Rab1B and MMP9 are combined into a new risk model, it has a remarkably better prediction of prognosis than each protein alone. In conclusion, Rab1B and MMP9 are potential prognostic biomarkers and their combination significantly improves predictive power for survival and chemotherapy response in CRC patients.

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Section: Discussionmentioning

confidence: 99%

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer

Yang

Cui

Zeng

et al. 2017

Aging

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“…We also set out to identify membrane markers that would define and traffic with these uncoated cargo carriers. Rab1 is a small GTPase with two mammalian isoforms (Rab1a and Rab1b) that has been shown to regulate the transport of membranes and cargo from the ER to the Golgi (Galea et al, 2015;Martinez et al, 2016;Pind et al, 1994;Plutner et al, 1991;Slavin et al, 2011;Wilson et al, 1994). Rab1 (Ypt1p in yeast) plays an essential role in the secretory pathway, regulating cargo transport from the ER to the Golgi (Bacon et al, 1989;Yuan et al, 2017).…”

Section: Rab1 Regulates Release and Trafficking Of Cargo Carriers Fromentioning

confidence: 99%

Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport

Westrate

Hoyer

Nash

et al. 2020

Journal of Cell Science

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Secretory cargo is recognized, concentrated and trafficked from endoplasmic reticulum (ER) exit sites (ERES) to the Golgi. Cargo export from the ER begins when a series of highly conserved COPII coat proteins accumulate at the ER and regulate the formation of cargo-loaded COPII vesicles. In animal cells, capturing live de novo cargo trafficking past this point is challenging; it has been difficult to discriminate whether cargo is trafficked to the Golgi in a COPII-coated vesicle. Here, we describe a recently developed live-cell cargo export system that can be synchronously released from ERES to illustrate de novo trafficking in animal cells. We found that components of the COPII coat remain associated with the ERES while cargo is extruded into COPII-uncoated, non-ER associated, Rab1 (herein referring to Rab1a or Rab1b)-dependent carriers. Our data suggest that, in animal cells, COPII coat components remain stably associated with the ER at exit sites to generate a specialized compartment, but once cargo is sorted and organized, Rab1 labels these export carriers and facilitates efficient forward trafficking.This article has an associated First Person interview with the first author of the paper.

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“…In our attempt to characterize Rab1-KO or Rab5-KO phenotypes by using the Rab1-CKO or Rab5-CKO cell lines we had established, we first focused on widely recognized Rab1 and Rab5 functions. Rab1 is known to mainly localize at the Golgi and to be required for maintenance of Golgi morphology (Wilson et al, 1994;Alvarez et al, 2003;Haas et al, 2007;Aizawa and Fukuda, 2015), and it also regulates ER-to-Golgi vesicular trafficking (Schwaninger et al, 1992;Martinez et al, 2016;Westrate et al, 2020). To evaluate the effect of acute depletion of Rab1 protein on Golgi morphology, we immunocytochemically stained Rab1-CKO cells and control wildtype (WT) cells for GM130 (cis-Golgi marker) and GalNT2 (trans-Golgi marker).…”

Section: Resultsmentioning

confidence: 99%

Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system

Hatoyama

Homma

Hiragi

et al. 2021

Journal of Cell Science

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Two small GTPases, Rab1 and Rab5, are key membrane trafficking regulators that are conserved in all eukaryotes. Since they have recently been shown to be essential for cell survival and/or growth in cultured mammalian cells, thereby precluding the establishment of Rab1-knockout (KO) and Rab5-KO cells, it has been extremely difficult to assess the impact of complete Rab1 or Rab5 protein depletion on cellular functions. Here, we generated and analyzed conditional KO (CKO) cells for Rab1 (Rab1A/1B) and Rab5 (Rab5A/5B/5C) by using the auxin-inducible protein degradation system. Rab1-CKO and Rab5-CKO led to eventual cell death >18 h and >48 h, respectively, after auxin exposure. After acute Rab1 protein depletion, the Golgi stack and ribbon structures were completely disrupted, and ER-to-Golgi trafficking was severely inhibited. Moreover, we discovered a novel Rab1-depletion phenotype: perinuclear clustering of early endosomes and delayed transferrin recycling. In contrast, acute Rab5 protein depletion resulted in loss of early endosomes and late endosomes, but lysosomes appeared to be normal. We also observed a dramatic reduction in the intracellular signals of endocytic cargos via receptor-mediated or fluid-phase endocytosis in Rab5-depleted cells.

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Spatial-Temporal Study of Rab1b Dynamics and Function at the ER-Golgi Interface

Cited by 11 publications

References 51 publications

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer

Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport

Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system

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