Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKII<i>γ</i>

Aji, Kaisaier; Maimaijiang, Munila; Aimaiti, Abudusaimi; Rexiati, Mulati; Azhati, Baihetiya; Tusong, Hamulati; Cui, Lei

doi:10.1155/2016/1267480

Cited by 16 publications

(15 citation statements)

References 32 publications

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“…In the present study, α-SMA and SM22α were selected to identify tissues and cells, along with vimentin, desmin and CD90. α-SMA and SM22α are common specific markers in SMC studies (13)(14)(15). Results of IHC, RT-qPCR, immunofluorescence and the in-cell western assay demonstrated that these markers were present in SMCs.…”

Section: Discussionmentioning

confidence: 90%

“…Also, the present study could not guarantee that all the obtained cells were SMCs; to the best of the authors' knowledge, there is no precise method of identification and purification of SMCs. Previous studies summarize various methods for primary cell culture (1)(2)(3)15). Other studies have used these methods for isolation and culture of primary cells (4,5,7-9,33); however, the mechanisms of primary cell differentiation remain unclear.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to the traditional method, in the present study, smooth muscles were collected from the tumor-free esophagogastric junction (EGJ) of patients with esophageal carcinoma and the traditional method was improved by using an enzyme-injected (EI) method for SMC isolation at low temperature (4˚C) for an extended duration (14-24 h). Through comparative observation, it was identified that it was effective in isolating more adherent spindle cells and that the cells could proliferate in vitro for 3-8 generations of SMC primary culture, as indicated by identification with smooth muscle markers, including α-smooth muscle actin (α-SMA) (13)(14)(15), smooth muscle 22 α (SM22α) (14-16), vimentin (7,8), desmin (7,17) Enzyme-injected method of enzymatic dispersion at low temperature is effective for isolation of smooth muscle cells from human esophagogastric junction and CD90 (7,18). The present study identified improved processes for in vitro culture of SMCs obtained from the digestive tract and established a foundation for the study of primary esophageal motility disorders (PEMDs), gastroesophageal reflux diseases (GERDs) and tissue engineering of the esophagus.…”

Section: Introductionmentioning

confidence: 99%

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Enzyme‑injected method of enzymatic dispersion at low temperature is effective for isolation of smooth muscle cells from human esophagogastric junction

et al. 2020

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The present study was conducted to examine the feasibility of in vitro isolation and primary culture of smooth muscle cells (SMCs) from the esophagogastric junction (EGJ).Smooth muscles of EGJ were harvested from 23 patients with esophageal cancer during esophagostomy from January 2015 to December 2017. Enzymatic dispersion (ED) was performed for isolation. Collagenase II and Trypsin/EDTA were applied by enzyme injection (EI) into tissue fragments or immersion of tissue fragments into enzyme solution. Growth characteristics and proliferation [Cell Counting Kit-8 (CCK-8)] of cells were recorded for both smooth muscle cell medium (SMCM) and DMEM/F12 containing 10% newborn bovine serum (10%-F12). All ED methods could isolate primary cells; EI was the most effective method with low collagenase II concentration (0.5 mg/ml) at 4˚C for 14-24 h. Primary cells demonstrated mainly spindle-and long-spindle-shaped with 'hills and valleys' morphology. The CCK-8 assay in SMCM showed better proliferation results than in 10%-F12. After passaging for 4-8 generations in SMCM or 2-4 generations in 10%-F12, cells enlarged gradually with passages and lost spindle structures. mRNA and proteins of α-smooth muscle actin (α-SMA), smooth muscle 22 α (SM22α), vimentin, desmin, CD90 and proliferating cell nuclear antigen were detected in tissues and cells with different levels of expression. SMCs of esophageal circular muscle, esophageal longitudinal muscle, gastric circular muscle near sling in gastric bottom and gastric circular muscle near clasp in lesser gastric curvature, all cultured in 10%-F12, exhibited superior smooth muscle phenotypes compared with SMCs cultured in SMCM in terms of α-SMA, SM22α and vimentin expression. The EI method of ED at low temperature appears effective for isolation and primary culture of SMCs from human EGJ in vitro.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enzyme‑injected method of enzymatic dispersion at low temperature is effective for isolation of smooth muscle cells from human esophagogastric junction

et al. 2020

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“…ASCs are also prone to stimulate angiogenesis [36,37], an essential step for regenerative purposes. They can give rise to vascular cells, both smoothmuscle cells and endothelial cells [63][64][65]. They have also been shown to be able to differentiate into cells of ectodermic leaflet such as neuronal cells [39,[66][67][68].…”

Section: Ascs and Fatmentioning

confidence: 99%

The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds

Conese

Annacontini

Carbone

et al. 2020

Stem Cells International

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The continuous improvements in the field of both regenerative medicine and tissue engineering have allowed the design of new and more efficacious strategies for the treatment of chronic or hard-to-heal skin wounds, which represent heavy burden, from a medical and economic point of view. These novel approaches are based on the usage of three key methodologies: stem cells, growth factors, and biomimetic scaffolds. These days, the adipose tissue can be considered the main source of multipotent mesenchymal stem cells, especially adipose-derived stem cells (ASCs). ASCs are easily accessible from various fat depots and show an intrinsic plasticity in giving rise to cell types involved in wound healing and angiogenesis. ASCs can be found in fat grafts, historically used in the treatment of chronic wounds, and have been evaluated as such in both animal models and human trials, to exploit their capability of accelerating wound closure and inducing a correct remodeling of the newly formed fibrovascular tissue. Since survival and fitness of ASCs need to be improved, they are now employed in conjunction with advanced wound dressings, together with dermal regenerative templates and platelet-rich plasma (as a source of growth and healing factors). In this work, we provide an overview of the current knowledge on the topic, based on existing studies and on our own experience.

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“…The main tools for differentiating ASCs toward VSMCs include composing cell culture media and exerting mechanical stress in dynamic cell culture systems, similarly as for the re-differentiation of VSMCs. Examples of results obtained by various authors[10,11,[58][59][60][61][62][63][64][65][66][67] are summarized inTable 1. Culture conditions for differentiation of ASCs into VSMCs and the obtained markers of differentiation.…”

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confidence: 99%

Vascular Smooth Muscle Cells (VSMCs) in Blood Vessel Tissue Engineering: The Use of Differentiated Cells or Stem Cells as VSMC Precursors

Bačáková¹,

Trávníčková²,

Filová³

et al. 2018

Muscle Cell and Tissue - Current Status of Research Field

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Vascular smooth muscle cells (VSMCs) play important roles in the physiology and pathophysiology of the blood vessels. In a healthy adult organism, VSMCs are quiescent, but after a blood vessel injury, they undergo phenotypic modulation from the contractile phenotype to the synthetic phenotype, characterized by high activity in migration, proliferation and proteosynthesis. This behavior of VSMCs can lead to stenosis or obliteration of the vascular lumen. For this reason, VSMCs have tended to be avoided in the construction of blood vessel replacements. However, VSMCs are a physiological and the most numerous component of blood vessels, so their presence in novel advanced vascular replacements is indispensable. Either differentiated VSMCs or stem cells as precursors of VSMCs can be used in the reconstruction of the tunica media in these replacements. VSMCs can be obtained from blood vessels (usually from subcutaneous veins) taken surgically from the patients and can be expanded in vitro. During in vitro cultivation, VSMCs lose their differentiation markers, at least partly. These cells should therefore be re-differentiated by seeding them on appropriate scaffolds by composing cell culture media and by mechanical stimulation in dynamic bioreactors. Similar approaches can also be applied for differentiating stem cells, particularly adipose tissue-derived stem cells, toward VSMCs for the purposes of vascular tissue engineering.

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Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ

Cited by 16 publications

References 32 publications

Enzyme‑injected method of enzymatic dispersion at low temperature is effective for isolation of smooth muscle cells from human esophagogastric junction

Enzyme‑injected method of enzymatic dispersion at low temperature is effective for isolation of smooth muscle cells from human esophagogastric junction

The Role of Adipose-Derived Stem Cells, Dermal Regenerative Templates, and Platelet-Rich Plasma in Tissue Engineering-Based Treatments of Chronic Skin Wounds

Vascular Smooth Muscle Cells (VSMCs) in Blood Vessel Tissue Engineering: The Use of Differentiated Cells or Stem Cells as VSMC Precursors

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