Expression profile of plasma microRNAs in nonsyndromic cleft lip and their clinical significance as biomarkers

Zou, Jijun; Li, Jingyun; Li, Jun; Ji, Chenbo; Li, Qingping; Guo, Xirong

doi:10.1016/j.biopha.2016.05.033

Cited by 20 publications

(27 citation statements)

References 32 publications

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“…The selection standards were the same as we previously reported [11] and were as follows: infrequently reported in the literature, present in both samples and higher deep sequencing reads in miRBase. Bulge-Loop™ qRT-PCR was performed to validate these six differentially expressed miRNAs found in the miRNA microarray analysis.…”

Section: Resultsmentioning

confidence: 99%

“…The plasma samples were collected according to previously described methods [11]. Nonsyndromic cleft palate (NSCP) and nonsyndromic cleft lip with cleft palate (NSCLP) patients who underwent surgery and healthy children (normal face but who underwent surgery for hydrocele) participated in this study with their parent's consent at the Department of Burns and Plastic Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University in Nanjing, China.…”

Section: Methodsmentioning

confidence: 99%

“…GO enrichment analyses were performed online (http://geneontology.org/). KEGG pathway enrichment analyses of the predicted targets of the differentially expressed miRNAs were performed according to previously described methods [11]. KEGG pathway enrichment analyses were performed by the ShanghaiBio Corporation (SBC) analysis system, which uses clusterProfiler data from R/bioconductor software (http://www.r-project.org and http://www.bioconductor.org/) with public databases that include NCBI Entrez Gene (http://www.ncbi.nlm.nih.gov/gene), GO (http://www.geneontology.org), KEGG (http://www.genome.jp/kegg), and Biocarta (http://www.biocarta.com).…”

Section: Methodsmentioning

confidence: 99%

“…The identification of the miRNA-mRNA regulatory molecules is important to understand the regulatory mechanisms of CLP and CPO. The potential of plasma miRNAs to be potential non-invasive diagnostic biomarkers for nonsyndromic cleft lip in infants has been reported [11]. In this study, based on our Agilent human miRNA microarray chips, we identified differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate

Zou

et al. 2016

Oncotarget

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Plasma microRNAs (miRNAs) have recently emerged as a new class of regulatory molecules that influence many biological functions. However, the expression profile of plasma microRNAs in nonsyndromic cleft palate (NSCP) or nonsyndromic cleft lip with cleft palate (NSCLP) remains poorly investigated. In this study, we used Agilent human miRNA microarray chips to monitor miRNA levels in three NSCP plasma samples (mixed as the CP group), three NSCLP plasma samples (mixed as the CLP group) and three normal plasma samples (mixed as the Control group). Six selected plasma miRNAs were validated in samples from an additional 16 CP, 33 CLP and 8 healthy children using qRT-PCR. Using Venn diagrams, distinct and overlapping dysregulated miRNAs were identified. Their respective target genes were further assessed using gene ontology and pathway analysis. The results show that distinct or overlapping biological processes and signalling pathways were involved in CP and CLP. Our study showed that the common key gene targets reflected functional relationships to the Notch, Wnt, phosphatidylinositol and Hedgehog signalling pathways. Further studies should examine the mechanism of the potential target genes, which may provide new avenues for future clinical prevention and therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate

Zou

et al. 2016

Oncotarget

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“…Genomic studies of miRNAs in nsCL/P and nsCPO are only just starting. Two studies from the same research group analyzed miRNA expression in plasma of nsCL and nsCL/P patients (Li J. et al, 2016; Zou et al, 2016). Using a microarray screening method, 305 miRNAs in plasma of nsCL patients and 241 miRNAs in plasma of nsCL/P patients were found to be differentially expressed compared with healthy controls.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs in Palatogenesis and Cleft Palate

et al. 2017

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Palatogenesis requires a precise spatiotemporal regulation of gene expression, which is controlled by an intricate network of transcription factors and their corresponding DNA motifs. Even minor perturbations of this network may cause cleft palate, the most common congenital craniofacial defect in humans. MicroRNAs (miRNAs), a class of small regulatory non-coding RNAs, have elicited strong interest as key regulators of embryological development, and as etiological factors in disease. MiRNAs function as post-transcriptional repressors of gene expression and are therefore able to fine-tune gene regulatory networks. Several miRNAs are already identified to be involved in congenital diseases. Recent evidence from research in zebrafish and mice indicates that miRNAs are key factors in both normal palatogenesis and cleft palate formation. Here, we provide an overview of recently identified molecular mechanisms underlying palatogenesis involving specific miRNAs, and discuss how dysregulation of these miRNAs may result in cleft palate.

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Differentially expressed plasmatic microRNAs in Brazilian patients with Coronavirus disease 2019 (COVID-19): preliminary results

et al. 2022

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Expression profile of plasma microRNAs in nonsyndromic cleft lip and their clinical significance as biomarkers

Cited by 20 publications

References 32 publications

Assessment of differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate

Assessment of differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate

MicroRNAs in Palatogenesis and Cleft Palate

Differentially expressed plasmatic microRNAs in Brazilian patients with Coronavirus disease 2019 (COVID-19): preliminary results

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