Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation

Sakoda, Camila Pivari Pedroso; Toledo, Alessandra Choqueta de; Perini, Adenir; Pinheiro, Nathália; Hiyane, Meire Ioshie; Grecco, Simone dos Santos; Tibério, Iolanda de Fátima Lopes Calvo; Câmara, Niels Olsen Saraiva; Martins, Mílton de Arruda; Lago, João Henrique G.; Righetti, Renato Fraga; Prado, Carla M.

doi:10.1016/j.acthis.2016.07.001

Cited by 28 publications

(27 citation statements)

References 47 publications

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“…In previous studies, we demonstrated that this experimental model of allergic inflammation induced by repeated exposures of antigen showed several characteristics present in the pathophysiology of asthma, such as intense constriction, eosinophilic inflammation, oxidative stress, and remodeling of the extracellular matrix in the distal parenchyma (17,20,25,(37)(38)(39). Concerning the increase of Th1/Th2 and Th17 cytokines observed in the present study, increased levels of CD4+ and CD8+ cells activate immune responses in both humans and experimental mammalian models.…”

Section: Discussionsupporting

confidence: 71%

Effects of anti-IL17 on inflammation, remodeling, and oxidative stress in an experimental model of asthma exacerbated by LPS

Camargo

Righetti

Aristóteles

et al. 2018

Airway Cell Biology and Immunopathology

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Inflammation plays a central role in the development of asthma, which is considered an allergic disease with a classic Th2 inflammatory profile. However, cytokine IL-17 has been examined to better understand the pathophysiology of this disease. Severe asthmatic patients experience frequent exacerbations, leading to infection, and subsequently show altered levels of inflammation that are unlikely to be due to the Th2 immune response alone. This study estimates the effects of anti-IL-17 therapy in the pulmonary parenchyma in a murine asthma model exacerbated by LPS. BALB/c mice were sensitized with intraperitoneal ovalbumin and repeatedly exposed to inhalation with ovalbumin, followed by treatment with or without anti-IL-17. Twenty-four hours prior to the end of the 29-day experimental protocol, the two groups received LPS (0.1 mg/ml intratracheal OVA-LPS and OVA-LPS IL-17). We subsequently evaluated bronchoalveolar lavage fluid, performed a lung tissue morphometric analysis, and measured IL-6 gene expression. OVA-LPS-treated animals treated with anti-IL-17 showed decreased pulmonary inflammation, edema, oxidative stress, and extracellular matrix remodeling compared to the non-treated OVA and OVA-LPS groups (p < 0.05). The anti-IL-17 treatment also decreased the numbers of dendritic cells, FOXP3, NF-κB, and Rho kinase 1-and 2-positive cells compared to the non-treated OVA and OVA-LPS groups (p < 0.05). In conclusion, these data suggest that inhibition of IL-17 is a promising therapeutic avenue, even in exacerbated asthmatic patients, and significantly contributes to the control of Th1/Th2/Th17 inflammation, chemokine expression, extracellular matrix remodeling, and oxidative stress in a murine experimental asthma model exacerbated by LPS.

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Section: Discussionsupporting

confidence: 71%

Effects of anti-IL17 on inflammation, remodeling, and oxidative stress in an experimental model of asthma exacerbated by LPS

Camargo

Righetti

Aristóteles

et al. 2018

Airway Cell Biology and Immunopathology

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“…Considering the possible mechanisms, sakuranetin acts by the inhibition of ERK1/2, JNK, p38, and STAT3 activation in lungs. No alterations were found in the livers of treated animals [75].…”

Section: Antiinflammatory Activitymentioning

confidence: 80%

A Review on Sources and Pharmacological Aspects of Sakuranetin

Stompor

2020

Nutrients

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Sakuranetin belongs to the group of methoxylated flavanones. It is widely distributed in Polyomnia fruticosa and rice, where it acts as a phytoalexin. Other natural sources of this compound are, among others, grass trees, shrubs, flowering plants, cheery, and some herbal drugs, where it has been found in the form of glycosides (mainly sakuranin). Sakuranetin has antiproliferative activity against human cell lines typical for B16BL6 melanoma, esophageal squamous cell carcinoma (ESCC) and colon cancer (Colo 320). Moreover, sakuranetin shows antiviral activity towards human rhinovirus 3 and influenza B virus and was reported to have antioxidant, antimicrobial, antiinflammatory, antiparasitic, antimutagenic, and antiallergic properties. The aim of this review is to present the current status of knowledge of pro-health properties of sakuranetin.

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“…As the reticule should be placed in 10 random fields in the distal lung and 3–5 airway wall per animal at a magnification of 1000× and the number of positive cells was determined as the number of positive cells in each field divided by the number contacting the alveolar wall or airway area (10 4 μm 2 ). The researcher who performed the measurements was unaware of the study groups [38,39,40]. …”

Section: Methodsmentioning

confidence: 99%

A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice

Theodoro-Junior

Righetti

Almeida-Reis

et al. 2017

IJMS

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Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor-α (TNF-α), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management.

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Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation

Cited by 28 publications

References 47 publications

Effects of anti-IL17 on inflammation, remodeling, and oxidative stress in an experimental model of asthma exacerbated by LPS

Effects of anti-IL17 on inflammation, remodeling, and oxidative stress in an experimental model of asthma exacerbated by LPS

A Review on Sources and Pharmacological Aspects of Sakuranetin

A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice

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