Overexpression of GhSARP1 encoding a E3 ligase from cotton reduce the tolerance to salt in transgenic Arabidopsis

Liu, Yongchang; Zhang, Xinyu; Zhu, Shanfeng; Zhang, Hao; Li, Yanjun; Zhang, Tao; Sun, Jie

doi:10.1016/j.bbrc.2016.07.033

Cited by 12 publications

(8 citation statements)

References 32 publications

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“…The ubiquitin E3 ligase RHA2b promoted degradation of MYB30 [27], while MYB transcription factors were widely reported to be related to fiber development [28,29]. Furthermore, E3 ubiquitin ligase was also reported to be involved in stress tolerance in cotton, such as Verticillium dahlia [30], drought [31] and salt tolerance [32]. Overexpression of a rice SUMO E3 ligase gene OsSIZ1 in cotton enhanced drought and heat tolerance, and substantially improved fiber yields in the field under reduced irrigation and rainfed farming conditions [33].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association analysis reveals loci and candidate genes involved in fiber quality traits in sea island cotton (Gossypium barbadense)

Zhu

Song

et al. 2020

BMC Plant Biol

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Background Sea island cotton (Gossypium barbadense) has markedly superior high quality fibers, which plays an important role in the textile industry and acts as a donor for upland cotton (G. hirsutum) fiber quality improvement. The genetic characteristics analysis and the identification of key genes will be helpful to understand the mechanism of fiber development and breeding utilization in sea island cotton. Results In this study, 279 sea island cotton accessions were collected from different origins for genotyping and phenotyping fiber quality traits. A set of 6303 high quality single nucleotide polymorphisms (SNPs) were obtained by high-density CottonSNP80K array. The population characteristics showed that the sea island cotton accessions had wide genetic diversity and were clustered into three groups, with Group1 closely related to Menoufi, an original sea island cotton landrace, and Group2 and Group3 related to widely introduced accessions from Egypt, USA and Former Soviet Union. Further, we used 249 accessions and evaluated five fiber quality traits under normal and salt environments over 2 years. Except for fiber uniformity (FU), fiber length (FL) and fiber elongation (FE) were significantly decreased in salt conditions, while fiber strength (FS) and fiber micronaire (MIC) were increased. Based on 6303 SNPs and genome-wide association study (GWAS) analysis, a total of 34 stable quantitative trait loci (QTLs) were identified for the five fiber quality traits with 25 detected simultaneously under normal and salt environments. Gene Ontology (GO) analysis indicated that candidate genes in the 25 overlapped QTLs were enriched mostly in “cellular and biological process”. In addition, “xylem development” and “response to hormone” pathways were also found. Haplotype analyses found that GB_A03G0335 encoding an E3 ubiquitin-protein ligase in QTL TM6004 had SNP variation (A/C) in gene region, was significantly correlated with FL, FS, FU, and FE, implying a crucial role in fiber quality. Conclusions The present study provides a foundation for genetic diversity of sea island cotton accessions and will contribute to fiber quality improvement in breeding practice.

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Section: Discussionmentioning

confidence: 99%

Genome-wide association analysis reveals loci and candidate genes involved in fiber quality traits in sea island cotton (Gossypium barbadense)

Zhu

Song

et al. 2020

BMC Plant Biol

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“…Many plant genes are induced by biological and abiotic stresses, such as insects, fungi, drought, and high-salinity. Not only may these genes function in stress responses, but in stress tolerance as well [ 43 , 44 ]. Gene-expression patterns are often associated with gene function [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

et al. 2018

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BackgroundCucumber downy mildew is among the most important diseases that can disrupt cucumber production. Propamocarb, also known as propyl-[3-(dimethylamino)propyl]carbamate (PM), is a systemic carbamate fungicide pesticide that is widely applied in agricultural production because of its high efficiency of pathogens control, especially cucumber downy mildew. However, residual PM can remain in cucumbers after the disease has been controlled. To explore the molecular mechanisms of PM retention, cucumber cultivars ‘D9320’ (with the highest residual PM content) and ‘D0351’ (lowest residual PM content) were studied. High-throughput tag-sequencing (Tag-Seq) results showed that the CsDIR16 gene was related to PM residue, which was verified using transgenic technology.ResultsWe investigated the activity of a dirigent cucumber protein encoded by the CsDIR16 in gene response to stress induced by PM treatment. Gene-expression levels of CsDIR16 were up-regulated in the fruits, leaves, and stems of ‘D0351’ plants in response to PM treatment. However, in cultivar ‘D9320’, CsDIR16 levels were down-regulated in the leaves and stems after PM treatment, with no statistically significant differences observed in the fruits. Induction by jasmonic acid, abscisic acid, polyethylene glycol 4000, NaCl, and Corynespora cassiicola Wei (Cor) resulted in CsDIR16 up-regulation in ‘D0351’ and ‘D9320’. Expression after salicylic acid treatment was up-regulated in ‘D0351’, but was down-regulated in ‘D9320’. CsDIR16 overexpression lowered PM residues, and these were more rapidly reduced in CsDIR16(+) transgenic ‘D9320’ plants than in wild-type ‘D9320’ and CsDIR16(−) transgenic plants.ConclusionsAnalyses of the CsDIR16-expression patterns in the cucumber cultivars with the highest and lowest levels of PM residue, and transgenic validation indicated that CsDIR16 plays a positive role in reducing PM residues. The findings of this study help understand the regulatory mechanisms occurring in response to PM stress in cucumbers and in establishing the genetic basis for developing low-pesticide residue cucumber cultivars.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1236-2) contains supplementary material, which is available to authorized users.

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“…In cotton ( Gossypium hirsutum ), the RING finger E3 ligase SALT-ASSOCIATED RING FINGER PROTEIN (GhSARP1) is described as a negative regulator of salt stress tolerance [ 156 ]. When overexpressed in Arabidopsis, GhSARP1 enhanced salt stress sensitivity in transgenic plants during germination and seedling stages [ 156 ].…”

Section: Salt Stressmentioning

confidence: 99%

Plant E3 Ligases and Their Role in Abiotic Stress Response

Al-Saharin

Hellmann

Mooney

2022

Cells

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Plants, as sessile organisms, have limited means to cope with environmental changes. Consequently, they have developed complex regulatory systems to ameliorate abiotic stresses im-posed by environmental changes. One such system is the ubiquitin proteasome pathway, which utilizes E3 ligases to target proteins for proteolytic degradation via the 26S proteasome. Plants ex-press a plethora of E3 ligases that are categorized into four major groups depending on their structure. They are involved in many biological and developmental processes in plants, such as DNA repair, photomorphogenesis, phytohormones signaling, and biotic stress. Moreover, many E3 ligase targets are proteins involved in abiotic stress responses, such as salt, drought, heat, and cold. In this review, we will provide a comprehensive overview of E3 ligases and their substrates that have been connected with abiotic stress in order to illustrate the diversity and complexity of how this pathway enables plant survival under stress conditions.

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Overexpression of GhSARP1 encoding a E3 ligase from cotton reduce the tolerance to salt in transgenic Arabidopsis

Cited by 12 publications

References 32 publications

Genome-wide association analysis reveals loci and candidate genes involved in fiber quality traits in sea island cotton (Gossypium barbadense)

Genome-wide association analysis reveals loci and candidate genes involved in fiber quality traits in sea island cotton (Gossypium barbadense)

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

Plant E3 Ligases and Their Role in Abiotic Stress Response

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