Biology of ICE Bs1 , an integrative and conjugative element in Bacillus subtilis

Auchtung, Jennifer M.; Aleksanyan, Naira; Bulku, Artemisa; Berkmen, Melanie B.

doi:10.1016/j.plasmid.2016.07.001

Cited by 54 publications

(82 citation statements)

References 102 publications

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“…When most of the bacteria harbor ICEBs1, the concentration of PhrI in the media is high, hence RapI is repressed and the transfer of ICEBs1 is repressed. This mechanism prevents the transfer of the ICE to cells that already contain the element (Auchtung et al, 2016). A similar mechanism of attenuation of conjugative transfer was observed in A. tumefaciens (Cho et al, 2009), where A C C E P T E D M A N U S C R I P T 21 entry exclusion prevents the acquisition of similar plasmids to the one already present in the strain.…”

Section: Ices With Unknown Functionsmentioning

confidence: 72%

“…It is conjugative and is regulated by two modes. One of these is by DNA damage, involving the RecA protein; RecA promotes activity of ImmA, an antirepressor which cleaves ImmR allowing excision and transfer (Auchtung et al, 2016;Bose and Grossman, 2011). The second mechanism relies on the cell density of potential donors.…”

Section: Ices With Unknown Functionsmentioning

confidence: 99%

“…Another interesting feature of ICEBs1, is its ability to promote transfer of some plasmids, previously considered to be non-mobilizable. The lack of a clear phenotype conferred by ICEBs1, has led researchers to hypothesize that the advantage it provides to hosts carrying it is an increase in genome plasticity (Auchtung et al, 2016).…”

Section: Ices With Unknown Functionsmentioning

confidence: 99%

“…fluoroquinolones) and DNA damaging compounds, among others (Aminov, 2011). The induction of transfer in SXT-R391 and ICEBs1 occur through RecAdependent excision (Auchtung et al, 2016;Johnson and Grossman, 2015;Wozniak and Waldor, 2010). In these examples, transfer of the ICEs is not modulated by an isolated trait, but by an integrated regulatory network, that assures the prevalence of the ICE in the environment.…”

Section: Host Regulatory Elementsmentioning

confidence: 99%

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Regulation of conjugative transfer of plasmids and integrative conjugative elements

Bañuelos-Vazquez

Tejerizo

Brom

2017

Plasmid

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Section: Ices With Unknown Functionsmentioning

confidence: 72%

Section: Ices With Unknown Functionsmentioning

confidence: 99%

Section: Ices With Unknown Functionsmentioning

confidence: 99%

Section: Host Regulatory Elementsmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of conjugative transfer of plasmids and integrative conjugative elements

Bañuelos-Vazquez

Tejerizo

Brom

2017

Plasmid

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“…The MobT relaxases are related to a family of RCR initiators involved in the intracellular replication and maintenance of small plasmids (Guglielmini et al, 2013; Ambroset et al, 2016). MobT encoded by ICE Bs1 is involved in both conjugative transfer and in replication of the excised ICE [for a review, see (Auchtung et al, 2016)]. It should be mentioned that most families of relaxases involved in the RCR of small plasmids or viruses are clearly distinct from and perhaps unrelated to the superfamilies of relaxases found in conjugative and mobilizable elements.…”

Section: Introductionmentioning

confidence: 99%

A Glimpse into the World of Integrative and Mobilizable Elements in Streptococci Reveals an Unexpected Diversity and Novel Families of Mobilization Proteins

et al. 2017

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Recent analyses of bacterial genomes have shown that integrated elements that transfer by conjugation play an essential role in horizontal gene transfer. Among these elements, the integrative and mobilizable elements (IMEs) are known to encode their own excision and integration machinery, and to carry all the sequences or genes necessary to hijack the mating pore of a conjugative element for their own transfer. However, knowledge of their prevalence and diversity is still severely lacking. In this work, an extensive analysis of 124 genomes from 27 species of Streptococcus reveals 144 IMEs. These IMEs encode either tyrosine or serine integrases. The identification of IME boundaries shows that 141 are specifically integrated in 17 target sites. The IME-encoded relaxases belong to nine superfamilies, among which four are previously unknown in any mobilizable or conjugative element. A total of 118 IMEs are found to encode a non-canonical relaxase related to rolling circle replication initiators (belonging to the four novel families or to MobT). Surprisingly, among these, 83 encode a TcpA protein (i.e., a non-canonical coupling protein (CP) that is more closely related to FtsK than VirD4) that was not previously known to be encoded by mobilizable elements. Phylogenetic analyses reveal not only many integration/excision module replacements but also losses, acquisitions or replacements of TcpA genes between IMEs. This glimpse into the still poorly known world of IMEs reveals that mobilizable elements have a very high prevalence. Their diversity is even greater than expected, with most encoding a CP and/or a non-canonical relaxase.

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Type IV Secretion in Gram-Negative and Gram-Positive Bacteria

2017

Current Topics in Microbiology and Immunology

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Type IV secretion systems (T4SSs) are versatile multiprotein nanomachines spanning the entire bacterial cell envelope in Gram-negative and Gram-positive bacteria. They play important roles through the contactdependent secretion of effector molecules into eukaryotic hosts and conjugative transfer of mobile DNA elements as well as contact-independent exchange of DNA with the extracellular milieu. In the last few years, many details on the molecular mechanisms of T4SSs have been elucidated. Exciting structures of T4SS complexes from Escherichia coli plasmids R388 and pKM101, Helicobacter pylori and Legionella pneumophila have been solved. The structure of the F-pilus was also reported and surprisingly revealed a filament composed of pilin subunits in 1:1 stoichiometry with phospholipid molecules. Many new T4SSs have been identified and characterized, underscoring the structural and functional diversity of this secretion superfamily. Complex regulatory circuits also have been shown to control T4SS machine production in response to host cell physiological status or a quorum of bacterial recipient cells in the vicinity. Here, we summarize recent advances in our knowledge of 'paradigmatic' and emerging systems, and further explore how new basic insights are aiding in the design of strategies aimed at suppressing T4SS functions in bacterial infections and spread of antimicrobial resistances.

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Biology of ICE Bs1 , an integrative and conjugative element in Bacillus subtilis

Cited by 54 publications

References 102 publications

Regulation of conjugative transfer of plasmids and integrative conjugative elements

Regulation of conjugative transfer of plasmids and integrative conjugative elements

A Glimpse into the World of Integrative and Mobilizable Elements in Streptococci Reveals an Unexpected Diversity and Novel Families of Mobilization Proteins

Type IV Secretion in Gram-Negative and Gram-Positive Bacteria

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