Type IV Secretion in Gram-Negative and Gram-Positive Bacteria

doi:10.1007/978-3-319-75241-9

Cited by 9 publications

(1 citation statement)

References 138 publications

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“…This island has been found in 60-80% of isolates and encodes a unique type IV secretion system (T4SS) (Dillard and Seifert 2001; Hamilton et al 2005; Callaghan et al 2017; Shockey 2019). T4SSs are a diverse subset of protein nanomachines that can be used for protein secretion, conjugation, DNA uptake, and in the case of N. gonorrhoeae , DNA secretion (Backert and Grohmann 2017). The gonococcal T4SS exports single-stranded DNA directly into the extracellular space independent of contact with a host or neighboring cell (Dillard and Seifert 2001; Hamilton et al 2005; Salgado-Pabón et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional and translational responsiveness of theNeisseria gonorrhoeaetype IV secretion system to conditions of host infections

Callaghan

Klimowicz

Shockey

et al. 2021

Preprint

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The type IV secretion system of Neisseria gonorrhoeae translocates single-stranded DNA into the extracellular space, facilitating horizontal gene transfer and initiating biofilm formation. Expression of this system has been observed to be low under laboratory conditions, and multiple levels of regulation have been identified. We used a translational fusion of lacZ to traD, the gene for the type IV secretion system coupling protein, to screen for increased type IV secretion system expression. We identified several physiologically relevant conditions, including surface adherence, decreased manganese or iron, and increased zinc or copper, which increase gonococcal type IV secretion system protein levels through transcriptional and/or translational mechanisms. These metal treatments are reminiscent of the conditions in the macrophage phagosome. The ferric uptake regulator, Fur, was found to repress traD transcript levels, but to also have a second role, acting to allow TraD protein levels to increase only in the absence of iron. To better understand type IV secretion system regulation during infection, we examined transcriptomic data from active urethral infection samples from five men. These data demonstrated differential expression of 20 of 21 type IV secretion system genes during infection, indicating upregulation of genes necessary for DNA secretion during host infection.

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Section: Introductionmentioning

confidence: 99%

Transcriptional and translational responsiveness of theNeisseria gonorrhoeaetype IV secretion system to conditions of host infections

Callaghan

Klimowicz

Shockey

et al. 2021

Preprint

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Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system

Purtschert-Montenegro

Cárcamo-Oyarce

Pinto‐Carbó

et al. 2022

Nat Microbiol

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Many bacteria utilize contact-dependent killing machineries to eliminate rivals in their environmental niches. Here we show that the plant root colonizer Pseudomonas putida strain IsoF is able to kill a wide range of soil and plant-associated Gram-negative bacteria with the aid of a type IVB secretion system (T4BSS) that delivers a toxic effector into bacterial competitors in a contact-dependent manner. This extends the range of targets of T4BSSs—so far thought to transfer effectors only into eukaryotic cells—to prokaryotes. Bioinformatic and genetic analyses showed that this killing machine is entirely encoded by the kib gene cluster located within a rare genomic island, which was recently acquired by horizontal gene transfer. P. putida IsoF utilizes this secretion system not only as a defensive weapon to kill bacterial competitors but also as an offensive weapon to invade existing biofilms, allowing the strain to persist in its natural environment. Furthermore, we show that strain IsoF can protect tomato plants against the phytopathogen Ralstonia solanacearum in a T4BSS-dependent manner, suggesting that IsoF can be exploited for pest control and sustainable agriculture.

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Structure and Function of the Dot/Icm T4SS

Dutka

Liu

Maggi

et al. 2023

Preprint

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TheLegionella pneumophilaDot/Icm type IV secretion system (T4SS) delivers effector proteins into host cells during infection. Despite its significance as a potential drug target, our current understanding of its atomic structure is limited to isolated subcomplexes. In this study, we used subtomogram averaging and integrative modeling to construct a nearly-complete model of the Dot/Icm T4SS accounting for seventeen protein components. We locate and provide insights into the structure and function of six new components including DotI, DotJ, DotU, IcmF, IcmT, and IcmX. We find that the cytosolic N-terminal domain of IcmF, a key protein forming a central hollow cylinder, interacts with DotU, providing insight into previously uncharacterized density. Furthermore, our model, in combination with analyses of compositional heterogeneity, explains how the cytoplasmic ATPase DotO is connected to the periplasmic complex via interactions with membrane-bound DotI/DotJ proteins. Coupled within situinfection data, our model offers new insights into the T4SS-mediated secretion mechanism.

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Type IV Secretion in Gram-Negative and Gram-Positive Bacteria

Cited by 9 publications

References 138 publications

Transcriptional and translational responsiveness of theNeisseria gonorrhoeaetype IV secretion system to conditions of host infections

Transcriptional and translational responsiveness of theNeisseria gonorrhoeaetype IV secretion system to conditions of host infections

Pseudomonas putida mediates bacterial killing, biofilm invasion and biocontrol with a type IVB secretion system

Structure and Function of the Dot/Icm T4SS

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