Bioprosthetic mesh of bacterial cellulose for treatment of abdominal muscle aponeurotic defect in rat model

Abstract

The use of meshes for treatment of hernias continues to draw attention of surgeons and the industry in the search of an ideal prosthesis. The purpose of this work is to use meshes manufactured from bacterial cellulose, evaluate their organic tissue interaction and compare with an expanded polytetrafluorethylene (ePTFE's) prosthesis used to repair acute defect of muscle aponeurotic induced in rats. Forty-five male Wistar rats were classified using the following criteria: (1) surgical repair of acute muscle aponeurotic defect with perforated bacterial cellulose film (PBC; n = 18); (2) compact bacterial cellulose film (CBC; n = 12) and (3) ePTFE; (n = 15). After postoperative period, rectangles (2 × 3 cm) including prosthesis, muscles and peritoneum were collected for biomechanical, histological and stereological analysis. In all cases, the maximum acceptable error probability for rejecting the null hypothesis was 5 %. Between PBC and CBC samples, the variables of strain (P = 0.011) and elasticity (P = 0.035) were statistically different. The same was found between CBC and ePTFE (elasticity, P = 0.000; strain, P = 0.009). PBC differed from CBC for giant cells (P = 0.001) and new blood vessels (P = 0.000). In conclusion, there was biological integration and biomechanical elasticity of PBC; therefore, we think this option should be considered as a new alternative biomaterial for use as a bio prosthesis.

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

“…Furthermore, BC has great retention moisture content ability and suitable mechanical properties. As a result of its similarity with skin, BC has been widely used in the reconstruction of this tissue …”

“…Silveira et al . developed two distinct types of BC meshes, a compacted one and a perforated one, and compared them with ePTFE meshes in terms of their in vivo tissue response.…”

Applications of bacterial-synthesized cellulose in veterinary medicine – a review

“…The use of prosthetic meshes in abdominal wall defects and hernias is a well-accepted practice leading to a reduced rate of postoperative recurrence (Silveira et al 2016). However, the type of prosthesis best suitable for this purpose is not well established.…”

“…However, the type of prosthesis best suitable for this purpose is not well established. There is a variety of materials for hernia repair; polypropylene (PP) (Zharikov et al 2018) and polytetrafluorethylene (PTFE) (Silveira et al 2016) are the most commonly used today. Although they have positive properties, drawbacks like chronic pain produced by fibrous tissue; inflammation of the surrounding tissues; reduced abdominal wall mobility; foreign body sensation at the implant site and infections are often noticed (Zharikov et al 2018).…”

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“…A delay in BM-MSC anchoring to the BCM 440 was observed when compared to adhesion in culture plates, and this anchoring onset was evident 441 in a few hours. According toSilveira et al (2016), the three-dimensional structure of BCM 442 nanofibers exhibits an arrangement similar to that of the collagen fibers of the extracellular443 matrix, and a surface with different pores can provide variable times for cell adhesion to the 444 biomaterial. 445 BM-MSC anchoring and proliferation on the BCM were evident on day 7 of culture with 446 grouped cells, and several cytoplasmic projections were evident in the BCM.…”