Expression Profiles, Characterization and Function of HbTCTP in Rubber Tree (Hevea brasiliensis)

Abstract

As a highly conserved protein, the translationally controlled tumor protein (TCTP) carries out vital roles in various life processes. In rubber tree, two TCTP genes, HbTCTP and HbTCTP1, were cloned, but only HbTCTP1 was studied in details. In this study, cis-acting regulatory elements, expression patterns, subcellular localization, interacting proteins, and antioxidant activity of HbTCTP were systematically analyzed. Besides the common cis-acting regulatory elements, HbTCTP promoter also harbored various known cis-elements that respond to hormone/stresses. Being consistent with the aforementioned results, HbTCTP was regulated by drought, low temperature, high salt, ethylene (ET), wounding, H2O2, and methyl jasmonate (MeJA) treatments. HbTCTP was expressed throughout different tissues and developmental stages of leaves. In addition, HbTCTP was associated with tapping panel dryness (TPD). HbTCTP was localized in the membrane, cytoplasm and the nucleus, and interacted with four proteins rubber elongation factor (REF), 17.5 kDa heat shock family protein, annexin, and REF-like stress related protein 1. Being similar to HbTCTP1, HbTCTP also indicated antioxidant activity in metal-catalyzed oxidation (MCO) system. Our results are useful for further understanding the molecular characterization and expression profiles of HbTCTP, but also lay a solid foundation for elucidating the function of HbTCTP in rubber tree.

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

“…For example, a recent study on maize leaves subjected to flooding stress, reported that TCTP was increased in response to flooding stress, possibly mediated by increased production of hydrogen peroxide (Chen et al 2014b). TCTP from the rubber tree (Hevea brasiliensis) was found to be regulated by drought, low temperature, high salt, ethylene, hydrogen peroxide or wounding (Deng et al 2016;Li et al 2013). This is consistent with the observation that overexpression of TCTP in Arabidopsis resulted in increased drought tolerance of the plants (Kim et al 2012a).…”

Molecular characterization, expression analysis and heterologous expression of two translationally controlled tumor protein genes from Cucumis sativus

“…To gain insight into CsTCTP1 and CsTCTP2 function, we detected their expression patterns in various tissues by qRT-PCR. Consistent with previous studies [ 22 , 23 ], CsTCTP1 and CsTCTP2 were expressed in all examined tissues (roots, stems, leaves and cotyledons). Among these tissues, CsTCTP1 was highly expressed in the stems of both sister cucumber lines B21-a-2-1-2 and B21-a-2-2-2, while CsTCTP2 was highly expressed in the stems of B21-a-2-1-2 and in the roots of B21-a-2-2-2 ( Fig 3 ).…”

“…TCTP is involved in responses to a wide range of stimuli, such as mercury, heat, cold, drought, salt, ABA, ET and pathogens [ 13 , 23 , 26 , 27 ]. To test whether overproduction of CsTCTP1 and CsTCTP2 can improve in vivo stress tolerance, CsTCTP1 and CsTCTP2 were cloned in pET30a and overexpressed in E .…”

Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness

“…Numerous molecular studies have led to the identification of TPD-regulated genes, such as HbMyb1, HbTOM20 and HbTCTP1 3 , 9 – 16 . Differential transcript abundance was observed for twenty-seven candidate genes, related to TPD occurrence, in latex and phloem tissues for ROS-scavenging, ethylene biosynthesis and signalling genes 6 .…”