Translocation of nanoparticles and Mycobacterium marinum across the intestinal epithelium in zebrafish and the role of the mucosal immune system

Løvmo, Signe Dille; Speth, Martin; Repnik, Urška; Koppang, Erling Olaf; Griffiths, Gareth; Hildahl, Jon

doi:10.1016/j.dci.2016.06.016

Cited by 30 publications

(26 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with that, a labelled antigen derived from a Yersinia ruckeri immersionvaccine is initially detected in the midintestine enterocytes of zebrafish larvae, followed by the spleen (Korbut et al, 2016). Bacteria are trafficked to the basal side of the intestine, where they subsequently colocalise with leukocytes, before eventually travelling to the liver and spleen (Løvmo et al, 2017). Bacteria are trafficked to the basal side of the intestine, where they subsequently colocalise with leukocytes, before eventually travelling to the liver and spleen (Løvmo et al, 2017).…”

Section: Adaptive Immunity and The Gi Tractsupporting

confidence: 67%

“…In adult zebrafish orally infected with Mycobacterium marinum, bacteria are taken up into vacuoles by antigen-sampling cells, which make up the majority of the epithelium of the posterior midintestine. Bacteria are trafficked to the basal side of the intestine, where they subsequently colocalise with leukocytes, before eventually travelling to the liver and spleen (Løvmo et al, 2017).…”

Section: Adaptive Immunity and The Gi Tractmentioning

confidence: 99%

See 1 more Smart Citation

The zebrafish as a model for gastrointestinal tract–microbe interactions

Flores

Nguyen

Odem

et al. 2020

Cellular Microbiology

View full text Add to dashboard Cite

The zebrafish (Danio rerio) has become a widely used vertebrate model for bacterial, fungal, viral, and protozoan infections. Due to its genetic tractability, large clutch sizes, ease of manipulation, and optical transparency during early life stages, it is a particularly useful model to address questions about the cellular microbiology of host-microbe interactions. Although its use as a model for systemic infections, as well as infections localised to the hindbrain and swimbladder having been thoroughly reviewed, studies focusing on host-microbe interactions in the zebrafish gastrointestinal tract have been neglected. Here, we summarise recent findings regarding the developmental and immune biology of the gastrointestinal tract, drawing parallels to mammalian systems. We discuss the use of adult and larval zebrafish as models for gastrointestinal infections, and more generally, for studies of host-microbe interactions in the gut. K E Y W O R D S Danio rerio, gastrointestinal tract, host-pathogen interactions, infection model, microbiome, microbiota, zebrafish

show abstract

Section: Adaptive Immunity and The Gi Tractsupporting

confidence: 67%

Section: Adaptive Immunity and The Gi Tractmentioning

confidence: 99%

The zebrafish as a model for gastrointestinal tract–microbe interactions

Flores

Nguyen

Odem

et al. 2020

Cellular Microbiology

View full text Add to dashboard Cite

show abstract

“…The first evidence for the existence of M cells in fish was found in rainbow trout, in which the M-like cells were shown to exhibit similar characteristics to mammalian M cells, exemplified by their morphology (with openly arranged microvilli) and their affinity for the lectin UEA-1 but not WGA ( 15 ). In zebrafish, M-like cells have not been yet described, but nanoparticles, and bacteria ( Mycobacterium marinum ) are taken up in the intestinal epithelium when administered via an oral route ( 16 ). Bath vaccination of teleost fish is effective against pathogenic bacteria such as Aeromonas salmonicida subsp.…”

Section: Introductionmentioning

confidence: 99%

A Novel Antigen-Sampling Cell in the Teleost Gill Epithelium With the Potential for Direct Antigen Presentation in Mucosal Tissue

et al. 2018

View full text Add to dashboard Cite

In mammals, M cells can take up antigens through mucosal surfaces of the gut and the respiratory tract. Since M cells are deficient of lysosomes and phagosomes, the antigens are directly delivered to the mucosa-associated lymphoid tissue (MALT) without degradation. In teleost fish, the entire body surface (gills, skin, and intestinal system) is covered by mucus; however, specific antigen-sampling cells have not yet been identified in their mucosal tissues. Here, we show that two phenotypes of antigen-sampling cells take up antigens through epithelial surfaces of the rainbow trout gill. One phenotype of antigen-sampling cells has features of monocyte/macrophage/dendritic cell-type cells; they have large vacuoles in the cytoplasm and express PTPRC (CD45), CD83, IL-1β, and IL-12p40b. The second phenotype exhibits similar characteristics to mammalian M cells; the corresponding cells bind the lectin UEA-1 but not WGA and show expression of M cell marker gene Anxa5. In contrast to mammalian M cells, teleost M-type cells were found to exhibit small vacuoles in their cytoplasm and to express almost all genes related to the “phagosome”, “lysosome,” and “antigen processing and presentation” pathways. Furthermore, MHC class II was constitutively expressed on a fraction of M-type cells, and this expression was significantly increased after antigen uptake, suggesting that the MHC class II is inducible by antigen stimulation. Here, we suggest that teleost M-type cells play a role in the phylogenetically primitive teleost immune system, similar to bona-fide M cells. In addition, the presence of MHC class II expression suggests an additional role in antigen presentation in the gills, which are an organ with high T cell abundance, especially in interbranchial lymphoid tissue. The present results suggest an unconventional antigen presentation mechanism in the primitive mucosal immune system of teleosts, which generally lack highly organized lymphoid tissues. Moreover, the results of this work may be valuable for the development of mucosal vaccines that specifically target M-type cells; mucosal vaccines significantly reduce working costs and the stress that is usually induced by vaccination via injection of individual fish.

show abstract

“…As opposed to Cu ions, CuNPs are too large to be taken up by ion, protein, or transepithelial transporters and possibly endocytosis (Shaw and Handy ). A feature that has long been exploited in the oral delivery of fish vaccines, is the ability of the intestinal epithelium in fish to take up much larger materials by endocytosis compared with the intestinal epithelium of mammals (Handy et al ; Løvmo et al ). Consequently, fish may be more vulnerable to dietary CuNP exposure relative to mammals.…”

Section: Introductionmentioning

confidence: 99%

Comparative tissue distribution and depuration characteristics of copper nanoparticles and soluble copper in rainbow trout (Oncorhynchus mykiss)

Lindh

Razmara

Bogart

et al. 2018

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

The continuously growing number of products containing nanoparticles (NPs) makes their presence in the environment inevitable, and given the well‐known toxicity of dissolved metals, concerns regarding the toxicity of metal‐based NPs have been raised. Whether metal‐based NPs present similar or different toxicological effects compared with metal salts is an emerging field. In the present study, rainbow trout were intraperitoneally injected with CuSO4 or copper NPs (CuNPs) to investigate tissue distribution and depuration characteristics. Fish injected with Cu showed an initial accumulation of Cu in the liver, kidney, gills, intestine, and carcass. The Cu concentration in the liver of CuNP‐injected fish increased over time. It appears as though CuNPs accumulated in the liver at a greater rate than they were excreted. In livers of fish injected with CuSO4, the Cu concentration appeared to increase and reach an equilibrium, suggesting that copper was accumulated and excreted at the same rate. The possibility that CuNPs can accumulate at a higher rate than it is excreted in the liver warrants further investigation. The present study demonstrates the possibility of dietary uptake of CuNPs because elevated Cu concentrations were observed in carcass, gills, kidney, and intestine of fish gavaged with CuNPs. In conclusion, the results suggest that dietary CuNPs are taken up by the gut and preferentially accumulate in the liver. Environ Toxicol Chem 2019;38:80–89. © 2018 SETAC

show abstract

Translocation of nanoparticles and Mycobacterium marinum across the intestinal epithelium in zebrafish and the role of the mucosal immune system

Cited by 30 publications

References 71 publications

The zebrafish as a model for gastrointestinal tract–microbe interactions

The zebrafish as a model for gastrointestinal tract–microbe interactions

A Novel Antigen-Sampling Cell in the Teleost Gill Epithelium With the Potential for Direct Antigen Presentation in Mucosal Tissue

Comparative tissue distribution and depuration characteristics of copper nanoparticles and soluble copper in rainbow trout (Oncorhynchus mykiss)

Contact Info

Product

Resources

About