Low Expression of CDK5 and p27 Are Associated with Poor Prognosis in Patients with Gastric Cancer

Abstract

Several previous studies have demonstrated that CDK5 or p27 expression in gastric cancer are associated with overall survival. We have previously reported that tumor suppressive function of CDK5 is related to p27. The aim of this study was to investigate correlation between the clinicopathological parameters and overall survival with different CDK5/p27 expression statuses in 244 gastric cancer patients using immunohistochemistry. Low CDK5 expression was detected in 93 cases (38.11%) and low p27 in 157 cases (64.34%). The expression of CDK5 was significantly related to sex (P = 0.034) and Lauren's classification (P = 0.013). The expression of p27 was significantly related to sex (P = 0.012), differentiation (P = 0.003), TNM stage (P = 0.013) and lymph node metastasis (P = 0.001). Based on the combined expression of CDK5 and p27, we classified the patients into four subtypes: CDK5 Low/p27 Low (n = 69), CDK5 High/p27 Low (n = 88), CDK5 Low/p27 High (n = 24) and CDK5 High/p27 High (n = 63). The CDK5 Low/p27 Low expression was closely related to female (P = 0.026), diffuse type (P = 0.027) and lymph node metastasis (P = 0.010). The CDK5 Low/p27 Low patients displayed poorer survival in comparison with the rest of the patients in Kaplan-Meier analysis. No significant overall survival difference was observed among the patients with CDK5 High and/or p27 High expression. In the multivariate analysis, CDK5 and p27 co-expression status was identified as an independent prognostic factor for patients with gastric cancer.

Clinical significances of p27 in digestive tract cancers: a comprehensive analysis on immunohistochemistry staining, published literatures, microarray and RNA-seq data

“…In our present study, we also detected the expression pattern of p27 protein in DTCs and adjacent non-cancerous tissues using IHC. Our results showed that p27 protein was down-regulated in ESAC, ESCC, GC and CRC, which are consistent with preceding researches [ 14 , 25 , 49 , 75 ]. Then we further investigated the diagnostic ability of p27 protein in DTCs, and the results suggested that p27 protein could differentiate patients with DTCs from normal subjects to a degree.…”

“…The follow-up times ranged from 29 to 240 months, the cut-off values ranged from 0.03 to 0.75, and the sample sizes ranged from 23 to 1062. Among the 62 studies, 54 studies [ 8 , 10 – 62 ] followed survival, and 37 studies [ 8 , 13 , 15 – 20 , 24 , 28 – 30 , 36 , 39 , 40 , 44 – 47 , 49 – 51 , 54 , 55 , 57 – 61 , 63 – 70 ] investigated the relationships of p27 protein expression with lymph node metastasis, distant metastasis and pathology grading. Among the 54 survival-related studies, 17 articles provided HRs and 95% CIs directly, while 33 articles only provided Kaplan–Meier survival curves.…”

Cyclin-dependent kinase 5 acts as a promising biomarker in clear cell Renal Cell Carcinoma

“…Furthermore, the association between high CDK5 expression and poor prognosis has showed in other human malignancies, such as pancreatic cancer [12], lung cancer [13], thyroid carcinoma [14]. While, Sun Y et al reported that lower expression of CDK5 associated with poorer prognosis in gastric cancer [15]. Yet, there is no research about CDK5’s function which focusing on ccRCC patients.…”

The Ethanol Crude Extraction of Cyperus Rotundus Regulates Apoptosis-associated Gene Expression in HeLa Human Cervical Carcinoma Cells In Vitro

“…Treatment with CRE also inhibited ertain gene expression profiles, such as cell cycle-associated genes CDK1 (cyclindependent kinase 1), WEE1 (WEE1 G 2 checkpoint kinase), and CDC25A (cell division cycle associated 5). There are literature reports showing poor prognosis for gastric cancer patients with overexpressed carboxypeptidase A4 (CPA4), a zinc-containing exopeptidase (49). In this study, elevated CPA4 expression was detected in more than 50% of primary gastric cancer cases but it was weak or absent from the normal mucosa.…”