Comparative transcriptome analysis of the biocontrol strain Bacillus amyloliquefaciens FZB42 as response to biofilm formation analyzed by RNA sequencing

Kröber, Magdalena; Verwaaijen, Bart; Wibberg, Daniel; Winkler, Anderson M.; Pühler, Alfred; Schlüter, Andreas

doi:10.1016/j.jbiotec.2016.06.013

Cited by 40 publications

(34 citation statements)

References 58 publications

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“…Many researchers have reported disease mitigation with inoculation of various plant growth-promoting bacterial endophytes and more specifically with inoculation of Bacillus amyloliquefaciens (Kröber et al, 2016; Chen et al, 2016). Therefore, in this study, it was presumed that the inoculation of Bacillus amyloliquefaciens RWL-1 mitigated the deleterious effect of F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

Plant growth-promoting endophytic bacteria versus pathogenic infections: an example ofBacillus amyloliquefaciensRWL-1 andFusarium oxysporumf. sp.lycopersiciin tomato

Shahzad

Khan

Bilal

et al. 2017

PeerJ

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Fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. Currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. Here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic Bacillus amyloliquefaciens RWL-1 against pathogenic Fusarium oxysporum f. sp. lycopersici. The results revealed significant suppression of pathogenic fungal growth by Bacillus amyloliquefaciens in vitro. Further to this, we inoculated tomato plants with RWL-1 and F. oxysporum f. sp. lycopersici in the root zone. The results showed that the growth attributes and biomass were significantly enhanced by endophytic-inoculation during disease incidence as compared to F. oxysporum f. sp. lycopersici infected plants. Under pathogenic infection, the RWL-1-applied plants showed increased amino acid metabolism of cell wall related (e.g., aspartic acid, glutamic acid, serine (Ser), and proline (Pro)) as compared to diseased plants. In case of endogenous phytohormones, significantly lower amount of jasmonic acid (JA) and higher amount of salicylic acid (SA) contents was recorded in RWL-1-treated diseased plants. The phytohormones regulation in disease incidences might be correlated with the ability of RWL-1 to produce organic acids (e.g., succinic acid, acetic acid, propionic acid, and citric acid) during the inoculation and infection of tomato plants. The current findings suggest that RWL-1 inoculation promoted and rescued plant growth by modulating defense hormones and regulating amino acids. This suggests that bacterial endophytes could be used for possible control of F. oxysporum f. sp. lycopersici in an eco-friendly way.

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Section: Discussionmentioning

confidence: 99%

Plant growth-promoting endophytic bacteria versus pathogenic infections: an example ofBacillus amyloliquefaciensRWL-1 andFusarium oxysporumf. sp.lycopersiciin tomato

Shahzad

Khan

Bilal

et al. 2017

PeerJ

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“…A recent study by Kröber et al [294] in Bacillus amyloliquefaciens revealed differential gene expression in planktonic and biofilm cells. In this study, around 331 genes were significantly upregulated and 230 genes were downregulated in the transcriptome of bacterial biofilm as compared to planktonic population (Table 4).…”

Section: Genes Involved In Biofilm Formationplant Growth Promoting mentioning

confidence: 95%

Agriculturally important microbial biofilms: Present status and future prospects

2017

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Microbial biofilms are a fascinating subject, due to their significant roles in the environment, industry, and health. Advances in biochemical and molecular techniques have helped in enhancing our understanding of biofilm structure and development. In the past, research on biofilms primarily focussed on health and industrial sectors; however, lately, biofilms in agriculture are gaining attention due to their immense potential in crop production, protection, and improvement. Biofilms play an important role in colonization of surfaces - soil, roots, or shoots of plants and enable proliferation in the desired niche, besides enhancing soil fertility. Although reports are available on microbial biofilms in general; scanty information is published on biofilm formation by agriculturally important microorganisms (bacteria, fungi, bacterial-fungal) and their interactions in the ecosystem. Better understanding of agriculturally important bacterial-fungal communities and their interactions can have several implications on climate change, soil quality, plant nutrition, plant protection, bioremediation, etc. Understanding the factors and genes involved in biofilm formation will help to develop more effective strategies for sustainable and environment-friendly agriculture. The present review brings together fundamental aspects of biofilms, in relation to their formation, regulatory mechanisms, genes involved, and their application in different fields, with special emphasis on agriculturally important microbial biofilms.

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“…B. velezensis FZB42 was able to form biofilm for increasing biocontrol. This strain can also synthesize antifungal compounds, such as fengycin, bacillomycin D, difficidin, bacilysin, and amylocyclicin [34]. B. velezensis has been used in the biocontrol of both wheat, powdery mildew by Blumeria granminis and Wilt Disease Fusarium oxysporium [35].…”

Section: Evaluation Of Phytophthora Antagonism In Greenhouse By Endopmentioning

confidence: 99%

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

et al. 2020

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In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the antifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45-11.21%) and lower fatal rate (11.11-15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.

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Comparative transcriptome analysis of the biocontrol strain Bacillus amyloliquefaciens FZB42 as response to biofilm formation analyzed by RNA sequencing

Cited by 40 publications

References 58 publications

Plant growth-promoting endophytic bacteria versus pathogenic infections: an example ofBacillus amyloliquefaciensRWL-1 andFusarium oxysporumf. sp.lycopersiciin tomato

Plant growth-promoting endophytic bacteria versus pathogenic infections: an example ofBacillus amyloliquefaciensRWL-1 andFusarium oxysporumf. sp.lycopersiciin tomato

Agriculturally important microbial biofilms: Present status and future prospects

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

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