The prevalence, population structure and screening test specificity of penicillin-susceptible Staphylococcus aureus bacteremia isolates in Malmö, Sweden

Resman, Fredrik; Thegerström, John; Månsson, Fredrik; Ahl, Jonas; Tham, Johan; Riesbeck, Kristian

doi:10.1016/j.jinf.2016.05.011

Cited by 25 publications

(18 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand strains allocated to CC5, CC30, and CC45 were exclusively isolated from human samples (infection, skin, faeces, nose, and throat) and from food ( Figure 2) where human handling was very likely (ready to eat products). Furthermore these CCs on December 27, 2020 by guest http://aem.asm.org/ Downloaded from are widely described in literature to be found in human infections (48)(49)(50)(51). This is a further advantage of CC nomenclature as literature about them is broad (23,52,53) enabling to extend the scope beyond an egc enterotoxin caused SFPO.…”

Section: Inferring the Origin Of An Sfpo-involved Strainmentioning

confidence: 96%

Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Schwendimann

Merda

Berger

et al. 2021

Appl Environ Microbiol

View full text Add to dashboard Cite

Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic island vSaβ. These enterotoxins have been described as likely being involved in staphylococcal food poisoning outbreaks.The aim of the present study was to determine if whole genome data can be used for the prediction of staphylococcal egc enterotoxin production, particularly enterotoxin G (SEG) and enterotoxin I (SEI). For this purpose whole genome sequences of 75 Staphylococcus aureus (S. aureus) strains from different origins (food poisoning outbreaks, human, and animal) were investigated applying bioinformatics methods (phylogenetic analysis using the core genome and different alignments). SEG and SEI expression was tested in vitro using a sandwich ELISA method.Strains could be allocated to 14 different vSaβ types, each type being associated with a single clonal complex (CC). In addition the vSaβ type and CC were associated with the origin of the strain (human or cattle derived). The amount of SEG and SEI produced also correlated with the vSaβ type and the CC of a strain. The present results show promising indications that the in vitro production of SEG and SEI can be predicted based on the vSaβ type or CC of a strain.IMPORTANCE Beside the infection properties in human and animals, S. aureus can produce different enterotoxins in food. The enterotoxins can cause vomiting and diarrhea, often involving many people. Most of these outbreaks remain undiscovered as detection methods for enterotoxins are only available for a few enterotoxins, but not for the more recently discovered enterotoxin G (SEG) and I (SEI).In this study we show promising results that in vitro production of SEG and SEI can be predicted based on the whole genome sequencing data of a strain. In addition, this data could be used to find the source (human- or cattle-derived) of an outbreak strain, which is the key for a better understanding of the role SEG and SEI play in foodborne outbreaks caused by S. aureus.

show abstract

Section: Inferring the Origin Of An Sfpo-involved Strainmentioning

confidence: 96%

Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Schwendimann

Merda

Berger

et al. 2021

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In contrast with the extensive efforts to characterize MRSA, only a small number of studies have characterized the dynamics of all S. aureus subtypes, possibly due to the expectation that prevalence of penicillin susceptible S. aureus (PSSA) is extremely low 23 . However, recent reports from diverse sites describe a rising or surprisingly high prevalence of PSSA [24][25][26][27] . Whether this trend is associated with the decline in MRSA and how it impacts overall rates of S. aureus infection are unclear.…”

Section: Introductionmentioning

confidence: 99%

Increasing antibiotic susceptibility inStaphylococcus aureusin Boston, Massachusetts, 2000-2014: an observational study

Kanjilal

Sater

Thayer

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

“…R ecent publications have reported on the rise of penicillin-sensitive Staphylococcus aureus (PSSA). This trend has now been observed on several continents (1) and has been shown to involve bloodstream infections and other serious infections (2). This increase in the incidence of PSSA infections has also been mirrored by a decrease in methicillin-resistant S. aureus (MRSA) infection rates (3), which raises two important questions.…”

mentioning

confidence: 99%

Increasing Rates of Penicillin Sensitivity in Staphylococcus aureus

Butler‐Laporte

Lee

Cheng

2018

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The prevalence, population structure and screening test specificity of penicillin-susceptible Staphylococcus aureus bacteremia isolates in Malmö, Sweden

Cited by 25 publications

References 22 publications

Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of v Saβ Typing

Increasing antibiotic susceptibility inStaphylococcus aureusin Boston, Massachusetts, 2000-2014: an observational study

Increasing Rates of Penicillin Sensitivity in Staphylococcus aureus

Contact Info

Product

Resources

About