Potato wound-healing tissues: A rich source of natural antioxidant molecules with potential for food preservation

Abstract

The need for safe, effective preservatives is a prominent issue in the food and drug industries, reflecting demand for natural alternatives to synthetic chemicals viewed as harmful to consumers and the environment. Thus, this study determined the identities and scavenging capacities of antioxidant metabolites produced as a response to potato tuber wounding, using activity-guided fractionation of polar extracts from a Yukon Gold cultivar that had previously exhibited exceptionally high radical-scavenging activity. Activity-guided fractionation using the ABTS•+ radical scavenging assay and LC-MS with TOF-MS for compositional analysis of the most potent antioxidant fractions yielded identification of nine constituents: coumaroylputrescine; feruloylquinic acid; isoferuloylputrescine; ferulic acid; 22,25-dimethoxy-3-[[2,3,4-tri-O-methyl-6-O-(2,3,4,6-tetra-O-methyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-(3β)-lanost-9(11)-en-24-one; 4-(2Z)-2-decen-1-yl-5-[1-(4-hydroxyphenyl)decyl]-1,2-benzenediol; 8-[(2E)-3,7-dimethyl-2,6-octadien-1-yl]-5-hydroxy-2,8-dimethyl-6-(3-methyl-2-buten-1-yl)-2H-1-benzopyran-4,7(3H,8H)-dione; 3-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]-20-[(6-O-β-D-xylopyranosyl-β-D-glucopyranosyl)oxy]-dammar-24-en-19-al; (3β)-28-oxo-28-(phenylmethoxy)oleanan-3-yl 2-O-β-D-galactopyranosyl-3-O-(phenylmethyl)-, butyl ester β-D-glucopyranosiduronic acid. A positive correlation was observed between the scavenging activities and the polarities of the active fractions. The antioxidant capacities of the fractions were also characterised by monitoring the activity throughout a 45-minute assay period.

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

“…Antioxidants have wound healing properties and can protect tissues from oxidative damage31. CMLA possesses antioxidant activity as it increased the activities of antioxidant enzymes in tissue homogenates.…”

Taurine-loaded poly (ε-caprolactone)/gelatin electrospun mat as a potential wound dressing material: In vitro and in vivo evaluation

“…2 Different types of antioxidants have been used for wound healing to eliminate the destructive effects of free radicals. [3][4][5] Taurine (2-aminoethane sulfonic acid), a non-essential sulfur-containing amino acid found in large amounts in most mammalian tissues, is a powerful antioxidant which has positive effects on inflammation, cell proliferation, and collagen synthesis. 6 These include the cell membrane stabilization, modulation of the calcium level, osmoregulation, and antiapoptotic, antioxidant, and anti-inflammatory activities.…”

Control of restrictive supply chains : biomarkers as indicator for Erwinia infection on potato tuber

“…The PCA analysis of the thermo-desorption GC data indicates that specific volatiles are produced in non-infected tubers during healing. Dastmalchi et al (2016) identified nine constituents with high antioxidant properties during the wound healing process. These molecules have a high molecular weight and were extracted/measured with liquid chromatography.…”

“…These molecules have a high molecular weight and were extracted/measured with liquid chromatography. These molecules were not measured in our experiment as the sampling and the analysis methods differed from the ones used by Dastmalchi et al (2016).…”