Taurine-loaded poly (ε-caprolactone)/gelatin electrospun mat as a potential wound dressing material: In vitro and in vivo evaluation

Abstract

In this study, taurine (2-aminoethane sulfonic acid), an amino acid found in large amounts in most mammalian tissues, was incorporated with poly (ε-caprolactone) and gelatin in order to develop a drug-loaded composite wound dressing material. The composite mats from poly (ε-caprolactone)/ gelatin (1:1 (w/w)) solution incorporated with 3%, 5%, and 10% (w/w) of taurine were produced by electrospinning. The electrospun mats were evaluated regarding their morphology, wettability, water uptake capacity, water vapor transmission rate, tensile strength, and cellular response with L929 cell line. The mat containing 5% of taurine was chosen as the optimum dressing for in vivo study on the full-thickness excisional wounds of Wistar rats. The results showed that after 2 weeks, the wounds treated with the taurine-loaded dressing achieved a significant closure to nearly 92% compared with the sterile gauze, as control, which showed nearly 68% of wound closure. The histological examination of the wounds revealed that the wounds treated with the taurine-loaded dressing had densely packed collagen fibers with parallel alignment. Whereas, the sterile gauze-treated wounds had loosely packed collagen fibers with an irregular arrangement. Our results provided evidence supporting the possible applicability of the taurine-loaded wound dressings for successful wound treatment.

Exosome loaded alginate hydrogel promotes tissue regeneration in full‐thickness skin wounds: An in vivo study

“…An ideal skin substitute is required to provide an optimal healing property, including possessing an ECM‐like structure, high porosity, and permeability (Aderibigbe & Buyana, ; Baglio et al, ; Gantwerker & Hom, ). Regarding, scaffolds, different types of materials and structures such as hydrogels, films, and foams are being used to promote the healing process and protect skin against further damages (Aderibigbe & Buyana, ; Baglio et al, ; Farzamfar et al, ; Gantwerker & Hom, ). These dressings are produced from synthetic or natural polymers, including alginate, chitosan, collagen, and gelatin (Dhivya, Padma, & Santhini, ; Sood, Granick, & Tomaselli, ).…”

Development and Characterization of Hemicellulose-Based Films for Antibacterial Wound-Dressing Application

“…Therefore, there is a need to develop a wound dressing that can prolong the residence time of the antibiotics in the infected wound, absorb exudate, and maintain a moist environment around the wound area [15].Modern wound dressings (film wafers, hydrogels, and sponges) are designed to overcome the limitations of conventional wound dressings. The design and application of these dressings has been comprehensively reviewed [2,16], where it has been emphasized that the dressing materials must be biocompatible, exudate absorbing, allow water vapor transmission (WVTR), and possess antibacterial properties [16,17]. A simple method of preparation, excellent WVTR, transparency, and cost effectiveness are the attributes of good film dressings [18,19].Numerous synthetic and natural polymers and combinations thereof have been explored for making film dressings.…”

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

“…The porosity of the scaffolds was calculated using the following equation ().The tensile properties of the scaffold were determined by uniaxial tensile testing device (Santam, Karaj, Iran) at 1mm/ min extension rate. The wettability of electrospun scaffolds was determined by Sessile drop method using a contact angle measuring system (KRUS, Hamburg, Germany) (Farzamfar et al, 2017).…”