Simultaneous determination of nine coumarins in rat plasma by HPLC–MS/MS for pharmacokinetics studies following oral administration of Fraxini Cortex extract

Zhao, Mingwei; Ding, Weijing; Wang, Shuang; Wang, Qianqian; Du, Yingfeng; Xu, Huijun; Wang, Qiao; Jin, Shu‐Guang

doi:10.1016/j.jchromb.2016.04.042

Cited by 21 publications

(12 citation statements)

References 12 publications

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“…Previous studies conducted regular LC–MS/MS analysis to investigate the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin in rats after administration of plant extracts (Song, Zhang, Liu, & Jin, ; Sun et al, ; Wei et al, ; Zhang et al, ; Zhao et al, ; Zheng, Yin, Xu, & Wang, ). Thus far, no reports are available on simultaneous determination and comprehensive pharmacokinetic studies of these four compounds.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou

Yan

2018

Biomedical Chromatography

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A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for investigating the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin after oral administration of Daphne genkwa extract. Plasma samples were treated by protein precipitation with acetonitrile. Analytes were detected by triple-quadrupole MS/MS with an ESI source in negative selection reaction monitoring mode. The transitions of m/z 161 → 133 for umbelliferone, m/z 269 → 117 for apigenin, m/z 283 → 268 for genkwanin and m/z 299 → 284 for hydroxygenkwanin were confirmed for quantification. Chromatographic separation was conducted using an Eclipse XDB-C column, and the applied isocratic elution program allowed for simultaneous determination of the four analytes for a total run time of 2.5 min. The linearity was validated over the plasma concentration ranges of 1.421-1421 ng/mL for umbelliferone, 0.845-845 ng/mL for apigenin, 1.025-1025 ng/mL for genkwanin and 0.845-845 ng/mL for hydroxygenkwanin. The extraction recovery rate was >82.7% for each analyte. No apparent matrix effect was observed during the bioanalysis. After full validation, the proposed method was successfully applied to compare the pharmacokinetics of these analytes between normal and arthritic rats.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou

Yan

2018

Biomedical Chromatography

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“…Owing to its high sensitivity and specificity, ultra‐high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) has become a reliable and powerful tool for the determination of trace amounts of drugs in complex biological matrices. Several LC–MS/MS methods have been implemented for quantitation of aesculetin in rat or dog plasma (Wang et al, 2018; Zhao et al, 2016). However, these methods are either time consuming or involve tedious sample preparation.…”

Section: Introductionmentioning

confidence: 99%

LC–MS/MS for determination of aesculetin in rat plasma and its application to a pharmacokinetic study

Jiao

Qin

Wang

et al. 2021

Biomedical Chromatography

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Aesculetin, a coumarin compound present in the sancho tree and chicory, exhibits excellent antioxidant and anti‐inflammatory activities in the vascular and immune system. In this study, a rapid and sensitive ultra‐high performance liquid chromatography electrospray ionization–tandem mass spectrometry (UHPLC–ESI–MS/MS) method was established and validated for the determination of aesculetin in rat plasma. Plasma samples were prepared by protein precipitation with acetonitrile. Chromatographic separation was performed on an Acquity UPLC HSS T3 C18 column (2.1 × 100 mm, 1.8 μm) with gradient elution at a flow rate of 0.3 ml/min, using mobile phase consisting of 0.1% formic acid (A) and acetonitrile (B). Aesculetin and puerarin (internal standard) were detected by multiple reaction monitoring in negative ion mode. The method was fully validated according to the US Food and Drug Administration guidelines. The calibration curve was linear over the range of 2–1,000 ng/ml with correlation coefficient >0.9980. The carry‐over, matrix effect, extraction recovery, dilution effect, intra‐ and inter‐day precision and the accuracy were within acceptable limits. The method was then applied to a pharmacokinetic study of aesculetin in rats. After oral administration at doses of 5, 10 and 20 mg/kg, the plasma concentration reached peaks of 95.7, 219.9, 388.6 ng/ml at times of 1.22–1.78 h. The oral bioavailability was calculated as 15.6–20.3% in rat plasma. The result provided pre‐clinical information for further application of aesculetin.

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“…To investigate the pharmacokinetic characteristics of aesculin, aesculetin, fraxetin, fraxin and polydatin, several analytical methods based on chromatographic techniques for single or simultaneous quantification of analytes have been reported in recent years. High-performance liquid chromatography with ultraviolet (HPLC-UV), high-performance liquid chromatography quadrupole time of flight tandem mass (HPLC-QTQF-MS/MS), high-performance liquid chromatography with fluorescence detection (HPLC-FD), liquid chromatography tandem mass spectrometry (LC-MS/MS) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) methods were implemented for the determination and quantitation of aesculin, aesculetin, fraxetin, fraxin and polydatin in rat plasma and rabbit plasma [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. In addition, only a single report was retrieved online which described the pharmacokinetics of polydatin in beagle dog plasma [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Aesculin, Aesculetin, Fraxetin, Fraxin and Polydatin in Beagle Dog Plasma by UPLC-ESI-MS/MS and Its Application in a Pharmacokinetic Study after Oral Administration Extracts of Ledum palustre L.

et al. 2018

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A rapid, simple and sensitive ultra-performance liquid chromatography-electrospray-ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for the simultaneous determination of aesculin, aesculetin, fraxetin, fraxin and polydatin in beagle dog plasma for the first time. Plasma samples were pretreated by protein precipitation with methanol. Chromatographic separation was performed on an Acquity UPLC HSS T3 C18 column (2.1 mm × 100 mm, 1.8 μm) with gradient elution at a flow rate of 0.4 mL/min, using a mobile phase consisting of 0.1% formic acid (A) and acetonitrile (B). The analytes and IS were detected by multiple reaction monitoring (MRM) via negative ion mode with ion transitions of m/z 339.1–m/z 176.8 for aesculin, m/z 176.8–m/z 88.9 for aesculetin, m/z 206.8–m/z 192.1 for fraxetin, m/z 369.1–m/z 206.9 for fraxin, m/z 389.1–m/z 227.0 for polydatin and m/z 415.2–m/z 295.1 for puerarin. This method was validated according to the FDA guidelines and the results met the requirements of analysis. The calibration curves of analytes were linear with correlation coefficients more than 0.9980. The intra- and inter-day precisions were less than 15% and the accuracy was within ±15%. The maximum plasma concentration (Cmax) of aesculin, aesculetin, fraxetin, fraxin and polydatin was 46.75 ± 7.46, 209.9 ± 57.65, 369.7 ± 48.87, 67.04 ± 12.09 and 47.14 ± 12.04 ng/mL, respectively. The time to reach the maximum plasma concentration (Tmax) was 1.32 ± 0.38 h for aesculin, 1.03 ± 0.27 h for aesculetin, 0.94 ± 0.23 h for fraxetin, 0.83 ± 0.18 h for fraxin and 1.15 ± 0.15 h for polydatin. The results indicated that the absorption of aesculin might be slow in beagle dog plasma. This method was successfully applied for pharmacokinetics in beagle dog plasma after oral administration of the extracts of Ledum palustre L. at a dosage of 0.27 g/kg.

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Simultaneous determination of nine coumarins in rat plasma by HPLC–MS/MS for pharmacokinetics studies following oral administration of Fraxini Cortex extract

Cited by 21 publications

References 12 publications

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

LC–MS/MS for determination of aesculetin in rat plasma and its application to a pharmacokinetic study

Simultaneous Determination of Aesculin, Aesculetin, Fraxetin, Fraxin and Polydatin in Beagle Dog Plasma by UPLC-ESI-MS/MS and Its Application in a Pharmacokinetic Study after Oral Administration Extracts of Ledum palustre L.

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