Two-photon light-sheet nanoscopy by fluorescence fluctuation correlation analysis

Chen, Xuanze; Zong, Weijian; Li, Rongqin; Zeng, Zhiping; Zhao, Jingzhe; Xi, Peng; Liang-yi, Chen; Sun, Yujie

doi:10.1039/c6nr00324a

Cited by 33 publications

(22 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As such it is of interest to look into nondiffracting, propagation-invariant beams. Various reports have emerged over the past few years evaluating Bessel beams (Andilla et al, 2017;Fahrbach, Gurchenkov, Alessandri, Nassoy, & Rohrbach, 2013b;Fahrbach & Rohrbach, 2010Gao, Shao, Chen, & Betzig, 2014;Gohn-Kreuz & Rohrbach, 2016;Zhang, Phipps, Goodwin, & Werner, 2016;Zhao et al, 2014Zhao et al, , 2016 and Airy beams (Vettenburg et al, 2014) for LSFM, shown in Figure 5. Bessel beams are the most used as they are fairly easy to implement.…”

Section: Engineered Light Sheetsmentioning

confidence: 99%

“…Reportedly, a major advantage is the acquisition speed, that ranges between 200 to 1,000 planes/s in dithered mode, which is one order of magnitude more than with classic Bessel beams. The technique is undoubtedly very effective, but the set-up is quite complex and the use of a SLM reduces the temporal resolution when multicolor imaging is required since the SLM must be rapidly switched and synchronized with every color change (Zhao et al, 2016). The solution proposed by Zhao and coworkers (Zhao et al, 2016) is to use a combination of "nonelectronic" optical components to create ultrathin Bessel sheets of the same thickness regardless of the input laser light, as long as it is in the visible range.…”

Section: Engineered Light Sheetsmentioning

confidence: 99%

“…The technique is undoubtedly very effective, but the set-up is quite complex and the use of a SLM reduces the temporal resolution when multicolor imaging is required since the SLM must be rapidly switched and synchronized with every color change (Zhao et al, 2016). The solution proposed by Zhao and coworkers (Zhao et al, 2016) is to use a combination of "nonelectronic" optical components to create ultrathin Bessel sheets of the same thickness regardless of the input laser light, as long as it is in the visible range. The excitation light passes first through a slit to give it a line-shape, then through an annulus imaged to the back focal plane of the illumination objective.…”

Section: Engineered Light Sheetsmentioning

confidence: 99%

“…The lower light dose the sample receives, especially when recording 3D images, makes LSFM a gentler and less invasive modality to image photosensitive samples which are prone to photobleaching or phototoxicity. Moreover, thanks to its simple set‐up and widefield‐like detection, the technique lends itself to be combined with superresolution microscopy (STED/RESOLFT (Friedrich, Gan, Ermolayev, & Harms, ; Gohn‐Kreuz & Rohrbach, ; Hoyer et al, ; Scheul, Wang, & Vial, ), PALM/STORM/IML (Cella Zanacchi, Lavagnino, Faretta, Furia, & Diaspro, ; Cella Zanacchi et al, ; Greiss, Deligiannaki, Jung, Gaul, & Braun, ; Hu, Zimmerley, Li, Watters, & Cang, ; Palayret et al, ), SIM (Keller et al, ; Planchon et al, ; Zhao et al, )), techniques based on fluorescence fluctuation (FFS, FCS (Chen et al, ; Krieger, Singh, Garbe, Wohland, & Langowski, )) as well as other advanced microscopy and spectroscopy techniques (Raman (Rocha‐Mendoza et al, ), Tomography (Bassi, Schmid, & Huisken, )).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Technical implementations of light sheet microscopy

Zagato

Brans

Smedt

et al. 2018

Microscopy Res & Technique

View full text Add to dashboard Cite

Fluorescence-based microscopy is among the most successful methods in biological studies. It played a critical role in the visualization of subcellular structures and in the analysis of complex cellular processes, and it is nowadays commonly employed in genetic and drug screenings. Among the fluorescence-based microscopy techniques, light sheet fluorescence microscopy (LSFM) has shown a quite interesting set of benefits. The technique combines the speed of epi-fluorescence acquisition with the optical sectioning capability typical of confocal microscopes. Its unique configuration allows the excitation of only a thin plane of the sample, thus fast, high resolution imaging deep inside tissues is nowadays achievable. The low peak intensity with which the sample is illuminated diminishes phototoxic effects and decreases photobleaching of fluorophores, ensuring data collection for days with minimal adverse consequences on the sample. It is no surprise that LSFM applications have raised in just few years and the technique has been applied to study a wide variety of samples, from whole organism, to tissues, to cell clusters, and single cells. As a consequence, in recent years numerous set-ups have been developed, each one optimized for the type of sample in use and the requirements of the question at hand. Hereby, we aim to review the most advanced LSFM implementations to assist new LSFM users in the choice of the LSFM set-up that suits their needs best. We also focus on new commercial microscopes and "do-it-yourself" strategies; likewise we review recent designs that allow a swift integration of LSFM on existing microscopes.

show abstract

Section: Engineered Light Sheetsmentioning

confidence: 99%

Section: Engineered Light Sheetsmentioning

confidence: 99%

Section: Engineered Light Sheetsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Technical implementations of light sheet microscopy

Zagato

Brans

Smedt

et al. 2018

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

“…In particular, fluorescence intermittency (intensity blinking) is a common property of fluorophores, which has been used as an effective tool to break the optical diffraction limit. Super-resolution Optical Fluctuation Imaging (SOFI) is a super-resolution technique that achieves diffraction-unlimited imaging in all three dimensions through high-order cumulant analysis on the image sequence of intensity blinking fluorophores (Dertinger et al, 2009;Dertinger et al, 2010;Geissbuehler et al, 2012;Chen et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Lifetime super‐resolution optical fluctuation imaging

Zeng

Chen³

et al. 2019

Journal of Microscopy

Self Cite

View full text Add to dashboard Cite

Summary In this paper, we propose a promising super‐resolution imaging scheme in fluorescence lifetime domain (lifetime super‐resolution optical fluctuation imaging, ltSOFI). ltSOFI has the potential to obtain super‐resolution images by taking advantage of fluorescence lifetime blinking under wide‐field lifetime detection. The proof‐of‐concept for ltSOFI was demonstrated through numerical simulation of high‐order cumulant analysis on fluorescence lifetime blinking emitters. As a tentative experimental demonstration, we obtained super‐resolution lifetime imaging from time‐lapse FLIM recording of HeLa cells expressing a cAMP sensor using ltSOFI method. ltSOFI is expected to initiate a new dimension in the lifetime domain for blinking‐based super‐resolution microscopy. Lay Description We report on a promising super‐resolution imaging scheme in fluorescence lifetime domain (lifetime super‐resolution optical fluctuation imaging, ltSOFI). ltSOFI has the potential to obtain super‐resolution images by taking advantage of fluorescence lifetime blinking under wide‐field lifetime detection. Past advances in super‐resolution fluorescence microscopy primarily rely on the spatiotemporal modulation of the fluorescence intensity. Although the applications of the Q‐dot blinking have been discussed in the literature, most of the discussions have focused on the blinking of fluorescence intensity. Few studies have shown the possibility of super‐resolution imaging through fluorescence lifetime fluctuations. In this paper, we proposed the ltSOFI scheme that explored the possibility of super‐resolution reconstruction from the blinking of fluorescence lifetime. The proof‐of‐concept for ltSOFI was demonstrated through numerical simulation of high‐order cumulant analysis on fluorescence lifetime blinking emitters. As a tentative experimental demonstration, we obtained super‐resolution lifetime imaging from time‐lapse FLIM recording of HeLa cells expressing a cAMP sensor using ltSOFI method. The ltSOFI method is expected to initiate a new dimension in the lifetime domain for blinking‐based super‐resolution microscopy. Moreover, the existing fluorescence lifetime imaging microscopy and super‐resolution nanoscopy can benefit from the implementation of ltSOFI to significantly improve the imaging spatial resolution of fluorescence lifetime images. In addition, the proof‐of‐concept demonstration achieved by the numerical simulation and tentative experiment will provide a new perspective for obtaining fluorescence lifetime images with much finer details.

show abstract