Highly efficient energy transfer from quantum dot to allophycocyanin in hybrid structures

Karpulevich, Anastasia; Maksimov, Eugene G.; Sluchanko, Nikolai N.; Vasiliev, A. N.; Paschenko, V.Z.

doi:10.1016/j.jphotobiol.2016.03.048

Cited by 8 publications

(5 citation statements)

References 32 publications

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“…For both photosynthetic antennae, QD with optimal emission properties was used (QD530 or QD570 for PBSr and LHCII, respectively), prompting efficient energy transfer if only a close distance between the donor and the acceptor was assured. QDs were already shown to be able to donate energy to PBSr located in a short enough proximity. , The energy transfer in our system was proven by FLIM measurement, showing the fingerprint of FRET, the increase in acceptor τ. This technique was successfully used for proving protein–protein as well as protein–nanoparticle interaction …”

Section: Discussionmentioning

confidence: 53%

“…QDs were already shown to be able to donate energy to PBSr located in a short enough proximity. 32,33 The energy transfer in our system was proven by FLIM measurement, showing the fingerprint of FRET, the increase in acceptor τ. This technique was successfully used for proving protein−protein 51 as well as protein−nanoparticle interaction.…”

Section: ■ Discussionmentioning

confidence: 84%

“… 29 For example, it is expected that outside of the space of close mutual proximity, there is a very low chance for energy transfer between QDs and PBSr/LHCII. For energy transfer to occur, QDs and PBS need to be connected covalently 32 or assembled into electrostatically associated clusters. 33 The assembly studied here of QDs and PBSr/LHCII on a nanotube is a form of covalent connection that offers more specific options for the complex architecture.…”

Section: Introductionmentioning

confidence: 99%

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Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Sławski,

Maciejewski,

Szukiewicz

et al. 2023

ACS Omega

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The construction of artificial systems for solar energy harvesting is still a challenge. There needs to be a light-harvesting antenna with a broad absorption spectrum and then the possibility to transfer harvested energy to the reaction center, converting photons into a storable form of energy. Bioinspired and bioderivative elements may help in achieving this aim. Here, we present an option for light harvesting: a nanobiohybrid of colloidal, semiconductor quantum dots (QDs) and natural photosynthetic antennae assembled on the surface of a carbon nanotube. For that, we used QDs of cadmium telluride and cyanobacterial phycobilisome rods (PBSr) or light-harvesting complex II (LHCII) of higher plants. For this nanobiohybrid, we confirmed composition and organization using infrared spectroscopy, X-ray photoelectron spectroscopy, and high-resolution confocal microscopy. Then, we proved that within such an assembly, there is a resonance energy transfer from QD to PBSr or LHCII. When such a nanobiohybrid was further combined with thylakoids, the energy was transferred to photosynthetic reaction centers and efficiently powered the photosystem I reaction center. The presented construct is proof of a general concept, combining interacting elements on a platform of a nanotube, allowing further variation within assembled elements.

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Section: Discussionmentioning

confidence: 53%

Section: ■ Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

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Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Sławski,

Maciejewski,

Szukiewicz

et al. 2023

ACS Omega

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“…Taking into account the distance (counted from single steps in the multistep chain) between the donor and the final acceptor (from 10 to 12.5 nm, depending on the phycoerythrin trimer position to the nanocrystal), the phycoerythrin-QD bioconjugate is a very efficient photonic nanowire, in comparison to the DNA-scaffolded systems (Spillmann et al, 2013). Closer investigation of FRET between allophycocyanin and QDs demonstrated a significant increase in energy transfer efficiency after monomerization of allophycocyanin trimers by high temperature or sodium thiocyanate chaotropic conditions (Karpulevich et al, 2016). Moreover, covalent linking and stabilization of bionanohybrid resulted in even further enhancement of allophycocyanin fluorescence upon excitation of QDs.…”

Section: Quantum Dots -Fret Relays and Light-harvesting Complexesmentioning

confidence: 99%

Nanoparticles as energy donors and acceptors in bionanohybrid systems

Sławski

Grzyb

2019

Acta Biochim Pol

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The bionanohybrids are the junctions of at least two objects of different origin: abiotic and biotic. The abiotic part is a nanoparticle (often a fluorescent quantum dot), the biotical one may be a protein (especially fluorescent one or redox-active one), nucleic acid, carbohydrate as well as a simple organic molecule. When such a junction undergoes illumination, the energy transfer between the partners is possible. The nanoparticles, depending on their characteristics, may be donors, acceptors or mediators of the energy transfer. In most cases, the mechanism of the transfer is the Förster resonance energy transfer (FRET) or the electron transfer (ET). Here, we reviewed the newest achievements in the field with special attention paid to those bionanohybrids which allow FRET or ET. Such nanohybrids are important not only for exploration of the mechanism of the partner interaction but mainly for working out nanobiodevices for biosensing and nanotools for modern therapies.

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“…Furthermore, two-colour pump-probe polarisation anisotropy highlights the existence of delocalised excitonic states from large initial anisotropy which decays over around 550 fs (Womick and Moran 2009). Splitting generates a dramatic change in the absorption spectrum and pushes the fluorescence maximum toward the red (lower energy), increasing spectral overlap with the reaction centre absorption (Karpulevich et al 2016). Quantum beats are observed in APC and appear to decay within 35 fs and correspond to a Raman active frequency that is also resonant with the exciton energy gap.…”

Section: Antennae Derived From Cyanobacteriamentioning

confidence: 99%

Coherent phenomena in photosynthetic light harvesting: part two—observations in biological systems

et al. 2018

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Considerable debate surrounds the question of whether or not quantum mechanics plays a significant, non-trivial role in photosynthetic light harvesting. Many have proposed that quantum superpositions and/or quantum transport phenomena may be responsible for the efficiency and robustness of energy transport present in biological systems. The critical experimental observations comprise the observation of coherent oscillations or Bquantum beats^via femtosecond laser spectroscopy, which have been observed in many different light harvesting systems. Part Two of this review aims to provide an overview of experimental observations of energy transfer in the most studied light harvesting systems. Length scales, derived from crystallographic studies, are combined with energy and time scales of the beats observed via spectroscopy. A consensus is emerging that most long-lived (hundreds of femtoseconds) coherent phenomena are of vibrational or vibronic origin, where the latter may result in coherent excitation transport within a protein complex. In contrast, energy transport between proteins is likely to be incoherent in nature. The question of whether evolution has selected for these non-trivial quantum phenomena may be an unanswerable question, as dense packings of chromophores will lead to strong coupling and hence non-trivial quantum phenomena. As such, one cannot discern whether evolution has optimised light harvesting systems for high chromophore density or for the ensuing quantum effects as these are inextricably linked and cannot be switched off.

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Highly efficient energy transfer from quantum dot to allophycocyanin in hybrid structures

Cited by 8 publications

References 32 publications

Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Quantum Dots Assembled with Photosynthetic Antennae on a Carbon Nanotube Platform: A Nanohybrid for the Enhancement of Light Energy Harvesting

Nanoparticles as energy donors and acceptors in bionanohybrid systems

Coherent phenomena in photosynthetic light harvesting: part two—observations in biological systems

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