An ethylene and ROS-dependent pathway is involved in low ammonium-induced root hair elongation in Arabidopsis seedlings

C, Zhu; Yang, Na; Guo, Zhengfei; Meng, Qin; Gan, Longzhan

doi:10.1016/j.plaphy.2016.04.002

Cited by 22 publications

(6 citation statements)

References 40 publications

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“…Briefly, 21-d-old seedlings were treated with 0, 100, 140 or 180 mM NaCl for 7 d, tillering-stage (c. 50d-old) plants treated with 140 mM NaCl for 21 d, and bootingstage (c. 80-d-old) and flowering-stage (c. 105-d-old) soil-grown plants treated with 50 mM NaCl for 30 to 50 d. For salt tolerance phenotyping in wheat, the 14-d-old wheat seedlings were treated with 250 mM NaCl for 5 d, and their relative fresh weight against the untreated control plants were calculated. To examine the effects of ROS inhibitor on plant salt tolerance, the seedlings were first treated with 140 mM NaCl, followed by the application of 0.01 mM NADPH oxidase inhibitor diphenylene iodonium (DPI) at different time points (0 h, 1 d and 4 d; Zhu et al, 2016). For ROS sensitivity assay, the wheat seeds were germinated in distilled water for 3 d, and then treated with 0.5 mM or 50 lM methyl viologen (MV, N,N 0 -dimethyl-4,4 0 -bipyridinium dichloride) for 5 d to analyze their survival rate, or treated with 50 pM MV for 14 d to quantify the relative fresh weight against the untreated control seedlings.…”

Section: Plant Materials and Salt Treatmentmentioning

confidence: 99%

The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops

Cheng

Tang

et al. 2021

New Phytologist

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Salt stress triggers the overdose accumulation of reactive oxygen species (ROS) in crop plants, leading to severe oxidative damage to living tissues. MicroRNAs (miRNAs) act as master regulators orchestrating the stress responsive regulatory networks as well as salt tolerance. However, the fundamental roles of miRNAs in modulating salt tolerance in cereal crops, especially in salt-triggered ROS scavenging remain largely unknown. Through small RNA sequencing, a salt-responsive miRNA, miR172 was identified in rice. Further, by generating the miR172-overexpression or MIR172 gene loss-of-function mutant lines, the biological significance of miR172 and its downstream signaling pathways related to salt tolerance were defined. We demonstrated that miR172 is a positive regulator of salt tolerance in both rice and wheat. More interestingly, miR172a and miR172b, but not miR172c or miR172d are involved in salt stress response, emphasizing the functional differentiation within miR172 family members. Further evidence uncovers a novel miR172/IDS1 regulatory module that functions as a crucial molecular rheostat in maintaining ROS homeostasis during salt stress, mainly through balancing the expression of a group of ROS-scavenging genes. Our findings establish a direct molecular link between miRNAs and detoxification response in cereal crops for improving salt tolerance.

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Section: Plant Materials and Salt Treatmentmentioning

confidence: 99%

The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops

Cheng

Tang

et al. 2021

New Phytologist

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“…They integrate information from the endoplasmic reticulum, chloroplast, and mitochondria, thereby facilitating a coordinated stress response. Hence, it is evident that ROS participate in various ethylene-mediated plant responses [31]. In this study, we observed that under normal P supply conditions, with increasing exogenous ethephon concentration, the activity of SOD was inhibited, and the activity of POD was enhanced.…”

Section: Discussionmentioning

confidence: 58%

Effects of Exogenous Ethylene and Cobalt Chloride on Root Growth of Chinese Fir Seedlings under Phosphorus-Deficient Conditions

Zhang

Wang

Zheng

et al. 2021

Forests

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Studying the effects of different concentrations of ethephon on morphological and physiological changes in the roots of Chinese fir (Cunninghamia lanceolata Lamb. Hook.) seedlings under P deficiency can reveal the internal adaptive mechanisms of these plants under nutrient stress. Herein, we investigated the effects of different ethephon and cobalt chloride concentrations under normal P supply and P deficiency. A significant effect (p < 0.05) of exogenous additive application was observed on the development of Chinese fir root length, surface area, and volume. These root development indices showed maximum values when the ethephon concentration was 0.01 g kg−1 under normal P supply and P deficiency, and they were significantly different from those under 0.04 g kg−1 ethephon treatment. Similarly, the indices showed maximum values when CoCl2 concentration was 0.01 g kg−1 under P deficiency and was significantly different (p < 0.01) from those under 0.2 g kg−1 CoCl2 treatment. Under normal P supply, an increase in ethephon concentration caused superoxide dismutase (SOD; E.C. 1.15.1.1) activity to decrease and peroxidase (POD; E.C. 1.11.1.X) activity to increase gradually. Conversely, CoCl2 addition (0.01 g kg−1) promoted SOD and POD activities under P deficiency. There were no significant differences (p > 0.05) in malondialdehyde content of seedlings among ethephon or CoCl2 treatments. In conclusion, ethylene plays a significant role in adaptative mechanisms underlying stress resistance in plants, prompting them to respond to P starvation and improving seedlings’ tolerance to P-deficient conditions.

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“…This is consistent with our results ( Figure 6 ). Recently, ET was reported to participate in low NH 4 + -induced root hair elongation in Arabidopsis seedlings [ 121 ]. Additionally, IAA, CTK, and ABA have been reported to play critical roles in the plant response to fluctuating availability of useable N [ 108 , 122 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative Transcriptomic and Proteomic Analysis Reveals an Alternative Molecular Network of Glutamine Synthetase 2 Corresponding to Nitrogen Deficiency in Rice (Oryza sativa L.)

Liang

Yuan

et al. 2021

IJMS

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Nitrogen (N) is an essential nutrient for plant growth and development. The root system architecture is a highly regulated morphological system, which is sensitive to the availability of nutrients, such as N. Phenotypic characterization of roots from LY9348 (a rice variety with high nitrogen use efficiency (NUE)) treated with 0.725 mM NH4NO3 (1/4N) was remarkable, especially primary root (PR) elongation, which was the highest. A comprehensive analysis was performed for transcriptome and proteome profiling of LY9348 roots between 1/4N and 2.9 mM NH4NO3 (1N) treatments. The results indicated 3908 differential expression genes (DEGs; 2569 upregulated and 1339 downregulated) and 411 differential abundance proteins (DAPs; 192 upregulated and 219 downregulated). Among all DAPs in the proteome, glutamine synthetase (GS2), a chloroplastic ammonium assimilation protein, was the most upregulated protein identified. The unexpected concentration of GS2 from the shoot to the root in the 1/4N treatment indicated that the presence of an alternative pathway of N assimilation regulated by GS2 in LY9348 corresponded to the low N signal, which was supported by GS enzyme activity and glutamine/glutamate (Gln/Glu) contents analysis. In addition, N transporters (NRT2.1, NRT2.2, NRT2.3, NRT2.4, NAR2.1, AMT1.3, AMT1.2, and putative AMT3.3) and N assimilators (NR2, GS1;1, GS1;2, GS1;3, NADH-GOGAT2, and AS2) were significantly induced during the long-term N-deficiency response at the transcription level (14 days). Moreover, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that phenylpropanoid biosynthesis and glutathione metabolism were significantly modulated by N deficiency. Notably, many transcription factors and plant hormones were found to participate in root morphological adaptation. In conclusion, our study provides valuable information to further understand the response of rice roots to N-deficiency stress.

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An ethylene and ROS-dependent pathway is involved in low ammonium-induced root hair elongation in Arabidopsis seedlings

Cited by 22 publications

References 40 publications

The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops

The miR172/IDS1 signaling module confers salt tolerance through maintaining ROS homeostasis in cereal crops

Effects of Exogenous Ethylene and Cobalt Chloride on Root Growth of Chinese Fir Seedlings under Phosphorus-Deficient Conditions

Integrative Transcriptomic and Proteomic Analysis Reveals an Alternative Molecular Network of Glutamine Synthetase 2 Corresponding to Nitrogen Deficiency in Rice (Oryza sativa L.)

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