Comparative Study of Adipose-Derived Stem Cells From Abdomen and Breast

Guneta, Vipra; Tan, Nguan Soon; Sugii, Shigeki; Lim, Thiam Chye; Wong, Teck Neng; Choong, Cleo

doi:10.1097/sap.0000000000000797

Cited by 19 publications

(17 citation statements)

References 44 publications

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“…The ASC sample phenotype was characterized as CD29+/CD44+/CD105+/CD45-. The results of surface markers were consistent with those of other studies [Guneta et al, 2016;Kim et al, 2017], and the cells isolated were confirmed to be ASCs. The results showed reduced expression of CD105 in WAT-ASCs compared to BAT-ASCs.…”

Section: Discussionsupporting

confidence: 91%

Impact of Aging on the Characterization of Brown and White Adipose Tissue-Derived Stem Cells in Mice

Zhang

Wang

et al. 2020

Cells Tissues Organs

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Adipose tissue enriched with adipose tissue-derived stem cells (ASCs) is often used for stem cell-based therapies. However, the characteristics of ASCs from different types of adipose tissue have varying biochemical and functional properties. We aimed to investigate how age affected the biological and functional characteristics of ASCs from brown (BAT) and white adipose tissue (WAT). ASCs were obtained and cultured from mouse BAT and WAT at different ages: young (2 months of age) and older mice (22 months of age). Mesenchymal markers were characterized by flow cytometry, and cell proliferation, apoptosis, differentiation potential, senescence, and metabolism were then determined. The percentage of WAT was higher in elderly mice, and the percentage of BAT was higher in young mice. All ASC sample phenotypes were characterized as CD29+/CD44+/CD105+/CD45-; the proliferation rate was not statistically different among all age groups. However, the number of senescent cells and the percentage of apoptosis in elderly mouse ASCs were significantly increased, and the ability of osteogenic and lipogenic differentiation was decreased in these same animals. In addition, ASCs from young mice were more inclined to undergo osteogenic differentiation, especially BAT-ASCs, whose gene expression of fat-consuming components was also significantly higher than of WAT-ASCs. The results indicated that ASCs derived from both WAT and BAT possessed different characteristics of fat metabolism and cell differentiation relative to the osteo-and adipolineages. In particular, because BAT-ASCs from young mice contributed to fat consumption, if used for cell grafting, they may potentially be attractive vehicles for treating obesity.

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Section: Discussionsupporting

confidence: 91%

Impact of Aging on the Characterization of Brown and White Adipose Tissue-Derived Stem Cells in Mice

Zhang

Wang

et al. 2020

Cells Tissues Organs

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“…Whereas stem cells from the bone marrow are well characterized for their radiation sensitivity [ 16 ], studies on ADSCs are lacking or are carried out with mice as the host [ 17 ]. In addition, there are characteristic differences between adult stem cells isolated from adipose tissues of different origins, including growth kinetics [ 28 , 29 ]—a factor that may be responsible for tissue-specific radiation sensitivities [ 27 ]. From this reasoning, extensive analyses of underlying IR-induced mechanisms in ADSCs from different origins have to be performed in the future to engineer reliable cellular therapy options in radiation oncology.…”

Section: Discussionmentioning

confidence: 99%

Radiation Sensitivity of Adipose-Derived Stem Cells Isolated from Breast Tissue

Baaße

Machoy

Juerß

et al. 2018

IJMS

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Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology.

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“…18 Noteworthy is also the observation by Asterholm et al 19 that expression of inflammatory factors in adipocytes is required for adequate adipose tissue remodeling in response to energy intake. Noteworthy is also the observation by Guneta et al 20 who demonstrated marked differences between MSCs from breast fat and abdominal fat and it cannot be ruled out that ATMSCs-dependent graft survival depends on depot of origin. 21 …”

Section: Discussionmentioning

confidence: 99%