Survival and Inflammatory Response in Adipose-derived Mesenchymal Stem Cell-enriched Mouse Fat Grafts

Abstract

Supplemental Digital Content is available in the text.

The Impact of Cell-Expansion and Inflammation on The Immune-Biology of Human Adipose Tissue-Derived Mesenchymal Stromal Cells

“…On top of that, the lipoaspirate is considered to be medical waste, making adipose tissue an ideal source of MSCs [6,7,13]. Since the number of clinical applications using AT-MSCs is increasing, it is of main importance that the safety and full definition of this cell population is addressed [4,[33][34][35]. In vitro expansion is of relevance to allow suitable amount of AT-MSCs for transplantation.…”

Contemporary Review: The Use of Adipocyte-Derived Mesenchymal Stem Cells in Pathologies of the Foot and Ankle

“…Several large number of studies have been published in animal models outlining the various mechanisms by which ADSCs act on cell signaling. 4,6,25,30,36,47 ADSCs exhibit multipotent differentiation, rapid proliferation, and self-renewal properties. 26 They have been found to differentiate into endothelial cells and promote vascularization through vascular endothelial growth factor (VEGF), 20,73 and they exhibit potent immunomodulatory potential through both autocrine and paracrine mechanisms.…”

Evaluating full-thickness skin grafts in intraperitoneal onlay mesh position versus onlay position in mice

“…The use of transgenic mice with C57BL/6 isogeneic background, expressing luciferase driven by either a constitutively active promoter or NF-kB activation has been described previously. 16,17 In short, the firefly-derived luciferase gene was incorporated into either a plasmid behind NF-kBebinding sites or a CMVbased promoter, then injected into the pronucleus of fertilized C57BL/6 mouse eggs, thus producing mouse strains expressing either luciferase constitutively or through coexpression with NF-kB activation during inflammatory processes.…”