2016
DOI: 10.1038/srep23730
|View full text |Cite
|
Sign up to set email alerts
|

Modelling intracellular competition for calcium: kinetic and thermodynamic control of different molecular modes of signal decoding

Abstract: Frequently, a common chemical entity triggers opposite cellular processes, which implies that the components of signalling networks must detect signals not only through their chemical natures, but also through their dynamic properties. To gain insights on the mechanisms of discrimination of the dynamic properties of cellular signals, we developed a computational stochastic model and investigated how three calcium ion (Ca2+)-dependent enzymes (adenylyl cyclase (AC), phosphodiesterase 1 (PDE1), and calcineurin (… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
27
1

Year Published

2016
2016
2022
2022

Publication Types

Select...
7
2

Relationship

0
9

Authors

Journals

citations
Cited by 21 publications
(29 citation statements)
references
References 56 publications
(98 reference statements)
1
27
1
Order By: Relevance
“…The range of Ca 2+ rises necessary to induce LTP in the model (0.15–0.4 μmol.L −1 ) was similar to experimental estimations (0.1–0.3 μmol.L −1 ) 2 . We did not observe LTP for stimulations with Ca 2+ pulses of 1 s, which was consequent to the mechanisms of activation of CaN simulated 38 .…”
Section: Resultscontrasting
confidence: 60%
See 1 more Smart Citation
“…The range of Ca 2+ rises necessary to induce LTP in the model (0.15–0.4 μmol.L −1 ) was similar to experimental estimations (0.1–0.3 μmol.L −1 ) 2 . We did not observe LTP for stimulations with Ca 2+ pulses of 1 s, which was consequent to the mechanisms of activation of CaN simulated 38 .…”
Section: Resultscontrasting
confidence: 60%
“…Nevertheless, in the cells CaN always occurs as the heterodimer CNB/CNA 41 , consequently, its catalytic activation includes the binding of Ca 2+ to CNB prior to the binding of Ca 2+ /CaM to CNA 28 29 39 . The binding of Ca 2+ to CNB occurred with slow rate constants 29 in our model and limited the activation of CaN for brief signals 38 impairing LTP induction for Ca 2+ pulses of 1 s. Consequently, the kinetic aspects of CaN activation constrained the durations of the Ca 2+ transients 38 that promoted macroscopic LTP ( Fig. 2C ).…”
Section: Resultsmentioning
confidence: 83%
“…Typically in vitro assays for biological reactions are studied in equilibrium and in controlled conditions. However biological pathways might depend on the dynamics of the signals [38] and the convergence of multiple interactions. In our case AC activation might depend on the timing between CS-US, this dependence can differ greatly from the model, depending on the conditions inside the cell.…”
Section: Model Limitationsmentioning
confidence: 99%
“…AC1 responds to NMDA-mediated calcium elevations (and not calcium released from intracellular stores) and is mostly located close to the post-synaptic densities of dendritic spines [44]. AC1 is the predominant and neuro-specific calcium-stimulated adenylyl cyclase which is mostly expressed in dendrites, axons and at synapses [45]. At very high concentrations of calcium (> 10 µM ) AC1 is inhibited [46].…”
Section: Key Components and Governing Equations Of The Modelmentioning
confidence: 99%