Oxidative state and oxidative metabolism of the heart from rats with adjuvant-induced arthritis

Abstract

The aim of the present work was to investigate, in a more extensive way, the oxidative state and parameters related to energy metabolism of the heart tissue of rats using the model of adjuvant-induced arthritis. The latter is a model for the human arthritic disease. Measurements were done in the total tissue homogenate, isolated mitochondria and cytosolic fraction. The adjuvant-induced arthritis caused several modifications in the oxidative state of the heart which, in general, indicate an increased oxidative stress (+80% reactive oxygen species), protein damage (+53% protein carbonyls) and lipid damage (+63% peroxidation) in the whole tissue. The distribution of these changes over the various cell compartments was frequently unequal. For example, protein carbonyls were increased in the whole tissue and in the cytosol, but not in the mitochondria. No changes in GSH content of the whole tissue were found, but it was increased in the mitochondria (+33%) and decreased in the cytosol (-19%). The activity of succinate dehydrogenase was 77% stimulated by arthritis; the activities of glutamate dehydrogenase, isocitrate dehydrogenase and cytochrome c oxidase were diminished by 31, 25 and 35.3%, respectively. In spite of these alterations, no changes in the mitochondrial respiratory activity and in the efficiency of energy transduction were found. It can be concluded that the adjuvant-induced arthritis in rats causes oxidative damage to the heart with an unequal intracellular distribution. Compared to the liver and brain the modifications caused by arthritis in the heart are less pronounced on variables such as GSH levels and protein integrity. Possibly this occurs because the antioxidant system of the heart is less impaired by arthritis than that reported for the former tissues. Even so, the modifications caused by arthritis represent an imbalanced situation that probably contributes to the cardiac symptoms of the arthritis disease.

Yerba mate aqueous extract improves the oxidative and inflammatory states of rats with adjuvant-induced arthritis

“…8 In addition to affecting the articular cartilage, rheumatoid arthritis also evokes marked inflammatory responses and oxidative stress in other organs, such as vascular tissue, liver, brain and the heart. [9][10][11][12] In principle, the pronounced antioxidant activity of yerba mate should be able to attenuate the oxidative stress caused by rheumatoid arthritis. 4,5 Similarly, yerba mate should equally be able to attenuate the inflammatory state if one takes into account its well documented anti-inflammatory activity.…”

Merlot grape pomace hydroalcoholic extract improves the oxidative and inflammatory states of rats with adjuvant-induced arthritis

“…The liver of rats with adjuvant-induced arthritis, for example, presents increased rates of oxygen uptake, inhibition of gluconeogenesis and stimulation of glycolysis, alterations in the urea cycle and modifications in calcium homeostasis (Fedatto-Jr et al, 1999Utsunomiya, Scaliante, Bracht, & Ishii-Iwamoto, 2013;Yassuda-Filho et al, 2003). Substantial oxidative stress and molecular damage has also been demonstrated to occur in the plasma, liver, brain and heart of both arthritic patients (Mateen, Moin, Khan, Zafar, & Fatima, 2016) and experimental animals Comar et al, 2013;Schubert et al, 2016;Wendt et al, 2015).…”

Anti‐Inflammatory and Antioxidant Actions of Copaiba Oil Are Related to Liver Cell Modifications in Arthritic Rats

“…Rheumatoid arthritis is a systemic disease and in addition to the joints other organs are affected, such as brain, heart, lungs, and vascular tissue [McInnes and Schett, 2011]. The oxidative status is likewise changed in the serum blood of patients with rheumatoid arthritis and also in the liver, brain, and heart of rats with adjuvant arthritis [Lemarechal et al, 2006;Comar et al, 2013;Wendt et al, 2015;Bracht et al, 2016;Schubert et al, 2016].…”