Efficient production of polyhydroxyalkanoates (PHAs) from<i>Pseudomonas mendocina</i>PSU using a biodiesel liquid waste (BLW) as the sole carbon source

Chanasit, Wankuson; Hodgson, Brian; Sudesh, Kumar; Umsakul, Kamontam

doi:10.1080/09168451.2016.1158628

Cited by 40 publications

(32 citation statements)

References 55 publications

(84 reference statements)

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“…In response to this issue, much research has been conducted into the possibility of using industrial waste streams for fermentation processes to make PHAs economically favorable (Shahzad et al, 2013;Zakaria et al, 2013;Chanasit et al, 2016). Important examples are the utilization of waste plant oils, molasses from the sugar industry, lignocellulosic materials, oil palm shell, pressed fruit fiber, biodiesel waste, and waste animal oil.…”

Section: Introductionmentioning

confidence: 99%

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

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111

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Polyhydroxyalkanoates (PHAs) are a potential replacement for some petrochemical-based plastics. PHAs are polyesters synthesized and stored by various bacteria and archaea in their cytoplasm as water-insoluble inclusions. PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources. Such fermentation conditions have been optimized by industry to reduce the cost of PHAs produced commercially. Industrially, these biodegradable polyesters are derived from microbial fermentation processes utilizing various carbon sources. One of the major constraints in scaling-up PHA production is the cost of the carbon source metabolized by the microorganisms. Hence, cheap and renewable carbon substrates are currently being investigated around the globe. Plant and animal oils have been demonstrated to be excellent carbon sources for high yield production of PHAs. Waste streams from oil mills or the used oils, which are even cheaper, are also used. This approach not only reduces the production cost for PHAs, but also makes a significant contribution toward the reduction of environmental pollution caused by the used oil. Advancements in the genetic and metabolic engineering of bacterial strains have enabled a more efficient utilization of various carbon sources, in achieving high PHA yields with specified monomer compositions. This review discusses recent developments in the biosynthesis and classification of various forms of PHAs produced using crude and waste oils from the oil palm and fish industries. The biodegradability of the PHAs produced from these oils will also be discussed.

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Section: Introductionmentioning

confidence: 99%

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

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111

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“…This capability may require two separate biosynthetic pathways, for scl-PHA, mainly poly(3-hydroxybutyrate) and mcl-PHA production, with different PHA synthases (Chanasit et al . 2016 ). The poly(3-hydroxybutyrate) biosynthetic pathway consists of three enzymatic reactions, the condensation of two acetyl-coenzyme A (acetyl-CoA) molecules into acetoacetyl-CoA by the 3-ketoacylCoA thiolase PhaA, the NADPH-dependent reduction of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA by the (R)-specific acetoacetyl-CoA dehydrogenase/reductase PhaB and the (R)-3-hydroxybutyryl-CoA monomer polymerization into poly(3-hydroxybutyrate) by the poly(3-hydroxybutyrate) polymerase PhaC (Muhammadi, Muhammad and Shafqat 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Poly(3-hydroxybutyrate) hyperproduction by a global nitrogen regulator NtrB mutant strain of Paracoccus denitrificans PD1222

Olaya-Abril

Luque-Almagro

Manso

et al. 2017

FEMS Microbiology Letters

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Paracoccus denitrificans PD1222 accumulates short-length polyhydroxyalkanoates, poly(3-hydroxybutyrate), under nitrogen-deficient conditions. Polyhydroxybutyrate metabolism requires the 3-ketoacyl-CoA thiolase PhaA, the acetoacetyl-CoA dehydrogenase/reductase PhaB and the synthase PhaC for polymerization. Additionally, P. denitrificans PD1222 grows aerobically with nitrate as sole nitrogen source. Nitrate assimilation is controlled negatively by ammonium through the two-component NtrBC system. NtrB is a sensor kinase that autophosphorylates a histidine residue under low-nitrogen concentrations and, in turn, transfers a phosphoryl group to an aspartate residue of the response regulator NtrC protein, which acts as a transcriptional activator of the P. denitrificans PD1222 nasABGHC genes. The P. denitrificans PD1222 NtrB mutant was unable to use nitrate efficiently as nitrogen source when compared to the wild-type strain, and it also overproduced poly(3-hydroxybutyrate). Acetyl-CoA concentration in the P. denitrificans PD1222 NtrB mutant strain was higher than in the wild-type strain. The expression of the phaC gene was also increased in the NtrB mutant when compared to the wild-type strain. These results suggest that accumulation of poly(3-hydroxybutyrate) in the NtrB mutant strain of PD1222 responds to the high levels of acetyl-CoA that accumulate in the cytoplasm as consequence of its inability to efficiently use nitrate as nitrogen source.

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“…This might be attributed to substrate inhibition that cause osmotic stress and affect cellular metabolism that decreased enzymatic activities and PHA recovery [17]. The use of 1% (v/v) glycerol was reported as the optimal concentration for PHA production in Bacillus sphaericus [19], whereas 2% (v/v) glycerol was reported to be the optimal for PHA production by Pseudomonas mendocina PSU [20], Halomonas sp. SA8 [21], and Pandoraea sp.…”

Section: Discussionmentioning

confidence: 99%

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

2017

J App Biol Biotech

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Polyhydroxyalkalonates (PHAs) are biodegradable thermoplastics that are receiving immense attention as an alternative to petroleum derived plastics. The present study aimed to produce PHA using cheap and ecofriendly biodiesel waste glycerol as a substrate. Several PHA-producing bacterial isolates were isolated from different environmental samples and their efficacy for PHA production was assessed. Isolate Azu-IN1 showed the highest PHA production and was identified as Zobellella taiwanensis Azu-IN1 using 16S rRNA gene sequence and biochemical characterization. Factors affecting PHA production were optimized in batch fermentations. Supplementation of ammonium chloride as nitrogen source, methanol as an auxiliary carbon source, and agitation rate are the main limiting factors affecting PHA production. Maximum PHA production of 2.65 g/L at recovery yield of 50.3 (%), w/w) was achieved after 36 h in batch fermentation using optimized medium. Enhanced PHA production of 3.73 g/L with recovery yield of 61.7 % (w/w) was obtained in fed batch fermentation. The characteristics of extracted PHA were analyzed using Fourier Transform Infrared Spectroscopy (FTIR) 1 H and 13 C Nuclear Magnetic Resonance (NMR) spectroscopy. This is the first report on accumulation of PHA by Zobellella taiwanensis using glycerol as the sole carbon source.

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Efficient production of polyhydroxyalkanoates (PHAs) fromPseudomonas mendocinaPSU using a biodiesel liquid waste (BLW) as the sole carbon source

Cited by 40 publications

References 55 publications

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Poly(3-hydroxybutyrate) hyperproduction by a global nitrogen regulator NtrB mutant strain of Paracoccus denitrificans PD1222

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

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