Bioplastic products represent one of the most important eco-friendly alternatives to petrochemical plastics. Therefore, the study aims at producing polyhydroxyalkanoates (PHAs) using industrial wastes by the most potent bacterial isolate. Seventy-eight isolates were obtained from different localities in Arab Dairy Products Company (Panda, Qalyubia-Egypt). These isolates were subjected to qualitative and quantitative screening tests. Out of these isolates, bacterial isolate BZU-B2 showed the highest production of PHA. The selected strain was identified biochemically as Bacillus cereus by biochemical characterization. Nutritional and physiological factors that influence PHA productions were optimized. Temperature 37 ͦ C, whey concentration of 50%, and ammonium chloride 1.5g L were chosen as the best factors in achieving the highest production of PHA 1.63 g/L with a recovery yield of 33.42 (%, w/w) after 96h. The characteristics of extracted PHA were analyzed using FTIR-spectroscopy techniques. This study focused on improving the ability of Bacillus cereus to give a highly purified yield of PHA from whey (a low-cost carbon source).