A systematic study of the dissolution and relative bioavailability of four ginsenosides in the form of ultrafine granular powder, common powder and traditional pieces of Panax quinquefolius L, in vitro and in beagles

Chen, Sha; Xu, Hao-Qi; Jun, Zhang; Wang, Caixia; Peng, Li-Hua; Cheng, Jun; Liu, An

doi:10.1016/j.jep.2016.03.032

Cited by 14 publications

(7 citation statements)

References 28 publications

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“…Clinically, due to the large amount dosage use of traditional Chinese medicine with its poor effect of the ethanol or water extracts, ultrafine pulverizing technology, such as cryogenic grinding and air classifying grinding is applied to produce ultrafine powder (Li et al, 1991; Choi et al, 2012). It helps to increase the dissolution and improve the utilization ratio of the bioactive ingredients by cell breaking treatment (Chen et al, 2016; Xing et al, 2017). However, particle size distribution of their ultrafine powders only reaches to micron level.…”

Section: Discussionmentioning

confidence: 99%

Antiviral Properties of R. tanguticum Nanoparticles on Herpes Simplex Virus Type I In Vitro and In Vivo

Shen

et al. 2019

Front. Pharmacol.

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Herpes simplex virus type 1 (HSV-1), an enveloped DNA virus, plays a key role in varieties of diseases including recurrent cold sores, keratoconjunctivitis, genital herpes and encephalitis in humans. Great efforts have been made in developing more effective and less side-effects anti-herpes simplex virus agents, including traditional Chinese herbal medicines. In the present study, we evaluated the antiviral efficacy of Rheum tanguticum nanoparticles against HSV-1 in vitro and in vivo. R. tanguticum nanoparticles could inactivate the HSV-1 virions and block the viral attachment and entry into cells. Time-of-addition assay indicated that R. tanguticum nanoparticles could interfere with the entire phase of viral replication. Besides, R. tanguticum nanoparticles showed the ability to inhibit the mRNA expression of HSV-1 immediate early gene ICP4 and early gene ICP8 as well as the expression of viral protein ICP4 and ICP8. Moreover, R. tanguticum nanoparticles have been proved to protect mice against HSV-1 induced lethality by decreasing the viral load and alleviated pathological changes in brain tissues. In conclusion, we demonstrated that R. tanguticum nanoparticles could inhibit HSV-1 infection through multiple mechanisms. These results suggest that R. tanguticum nanoparticles may have novel roles in the treatment of HSV-1 infection.

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Section: Discussionmentioning

confidence: 99%

Antiviral Properties of R. tanguticum Nanoparticles on Herpes Simplex Virus Type I In Vitro and In Vivo

Shen

et al. 2019

Front. Pharmacol.

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“…Numerous in vitro studies on isolated cells have been conducted to uncover the molecular mechanisms of action of ginseng extracts and isolated ginsenosides (Supplemental Table S1 in Supplement 1). However, the concentrations of ginsenosides and the extracts used in these studies [32][33][34][35][36][37][38][39][40][41][42][43] were incompatibly higher than those detected in pharmacokinetic (PK) studies in humans [44,45] and animals orally administrated with ginseng preparations at the highest therapeutic doses [46][47][48][49][50][51] (Supplemental Table S2 in Supplement 1).…”

Section: Introductionmentioning

confidence: 85%

Network Pharmacology of Red Ginseng (Part I): Effects of Ginsenoside Rg5 at Physiological and Sub-Physiological Concentrations

Panossian¹,

Abdelfatah

Efferth

2021

Pharmaceuticals

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Numerous in vitro studies on isolated cells have been conducted to uncover the molecular mechanisms of action of Panax ginseng Meyer root extracts and purified ginsenosides. However, the concentrations of ginsenosides and the extracts used in these studies were much higher than those detected in pharmacokinetic studies in humans and animals orally administered with ginseng preparations at therapeutic doses. Our study aimed to assess: (a) the effects of ginsenoside Rg5, the major “rare” ginsenoside of Red Ginseng, on gene expression in the murine neuronal cell line HT22 in a wide range of concentrations, from 10−4 to 10−18 M, and (b) the effects of differentially expressed genes on cellular and physiological functions in organismal disorders and diseases. Gene expression profiling was performed by transcriptome-wide mRNA microarray analyses in HT22 cells after treatment with ginsenoside Rg5. Ginsenoside Rg5 exhibits soft-acting effects on gene expression of neuronal cells in a wide range of physiological concentrations and strong reversal impact at high (toxic) concentration: significant up- or downregulation of expression of about 300 genes at concentrations from 10−6 M to 10−18 M, and dramatically increased both the number of differentially expressed target genes (up to 1670) and the extent of their expression (fold changes compared to unexposed cells) at a toxic concentration of 10−4 M. Network pharmacology analyses of genes’ expression profiles using ingenuity pathway analysis (IPA) software showed that at low physiological concentrations, ginsenoside Rg5 has the potential to activate the biosynthesis of cholesterol and to exhibit predictable effects in senescence, neuroinflammation, apoptosis, and immune response, suggesting soft-acting, beneficial effects on organismal death, movement disorders, and cancer.

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“…At present, the classical extraction methods of CM and natural products mainly include decoction [25, 26], maceration [27–29], reflux [30, 31], percolation [21], steam distillation [32] and sublimation [33, 34]. With the development of modern separation science and equipment, some new technologies such as ultrasonic wave‐assisted extraction (UAE) [35–38], microwave‐assisted extraction (MAE) [39–42], supercritical fluid extraction (SFE) [43–47], enzyme‐assisted extraction (EAE) [48, 23, 49], membrane separation extraction (MSE) [50–57], ultrafine grinding technology (UGT) [58–64], microporous resin adsorption technology (MRAT) [65–70], semi‐bionic extraction (SBE) [71, 72], immune selective extraction (ISE) [73–77], molecular‐distillation technology [78–82], molecularly‐imprinted polymer extraction (MIPE) [83–88], UPE [89–93], liquid chromatographic extraction (LCE) [94–99], solid‐phase extraction (SPE) [100–107], liquid–liquid extraction (LLE) [108–112], etc. have been given birth to.…”

Section: Methodsmentioning

confidence: 99%

“…At present, the classical extraction methods of CM and natural products mainly include decoction [25,26], maceration [27][28][29], reflux [30,31], percolation [21], steam distillation [32] and sublimation [33,34]. With the development of modern separation science and equipment, some new technologies such as ultrasonic waveassisted extraction (UAE) [35][36][37][38], microwave-assisted extraction (MAE) [39][40][41][42], supercritical fluid extraction (SFE) [43][44][45][46][47], enzyme-assisted extraction (EAE) [48,23,49], membrane separation extraction (MSE) [50][51][52][53][54][55][56][57], ultrafine grinding technology (UGT) [58][59][60][61][62][63][64], microporous resin adsorption technology (MRAT) [65][66][67][68]…”

Section: Methodsmentioning

confidence: 99%

Comparison of different extraction methods of active ingredients of Chinese medicine and natural products

Li,

Chen

2024

J of Separation Science

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Like other traditional medicine in the world, Chinese traditional medicine (CTM) has a long history, which is a treasure of the combination of medicine and Chinese classical culture even more than 5000 years. For thousands of years, CTM has made great contributions to the reproduction and health of the Chinese people. It was an efficient therapeutic tool under the guidance of Chinese traditional medical theory, its source is generally natural products, but there are also a small number of it are natural products after some processing methods. In fact, the definition of Chinese medicine (CM) includes both traditional and new CM developed by modern technology. It is well known that the chemical composition of most CM and natural products is very complex, for example, a single herb may contain hundreds of different chemicals, including active ingredients, side effects, and even toxic ingredients. Therefore, the extraction process is particularly crucial for the quality and clinical efficacy of CM and natural products. In this work, a new classification method was proposed to divide the extraction technologies of CM and natural products into 21 kinds in recent years and analyze their status, advantages, and disadvantages. Then put forward a new technical route based on ultra‐high‐pressure extraction technology for rapid extraction else while removing harmful impurities and making higher utilization of CM and natural products. It is a useful exploration for the extraction industry of medicinal materials and natural products in the world.

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A systematic study of the dissolution and relative bioavailability of four ginsenosides in the form of ultrafine granular powder, common powder and traditional pieces of Panax quinquefolius L, in vitro and in beagles

Cited by 14 publications

References 28 publications

Antiviral Properties of R. tanguticum Nanoparticles on Herpes Simplex Virus Type I In Vitro and In Vivo

Antiviral Properties of R. tanguticum Nanoparticles on Herpes Simplex Virus Type I In Vitro and In Vivo

Network Pharmacology of Red Ginseng (Part I): Effects of Ginsenoside Rg5 at Physiological and Sub-Physiological Concentrations

Comparison of different extraction methods of active ingredients of Chinese medicine and natural products

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