Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell

Qi, Xianghui; Zhu, Jingfei; Yun, Jimmy; Lin, Jing; Qi, Yilin; Guo, Qi; Xu, Hong

doi:10.1016/j.jbiosc.2016.02.009

Cited by 16 publications

(8 citation statements)

References 23 publications

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“…JX-05. Co-biotransformation with both recombinant strains improved the xylitol yield by two times in comparison with wild Gluconobacter sp., possibly due to the utilization of other carbon sources along with xylose [51][52][53]. An endogenous aldose reductase gene GRE3 and a xylose transporter gene SUT1 gene were overexpressed in the industrial yeast strain of S. cerevisiae for xylitol production from agricultural biomass.…”

Section: Strain Improvementmentioning

confidence: 99%

Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism

Ahuja

Macho

Ewe

et al. 2020

Foods

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Xylitol is a white crystalline, amorphous sugar alcohol and low-calorie sweetener. Xylitol prevents demineralization of teeth and bones, otitis media infection, respiratory tract infections, inflammation and cancer progression. NADPH generated in xylitol metabolism aid in the treatment of glucose-6-phosphate deficiency-associated hemolytic anemia. Moreover, it has a negligible effect on blood glucose and plasma insulin levels due to its unique metabolism. Its diverse applications in pharmaceuticals, cosmetics, food and polymer industries fueled its market growth and made it one of the top 12 bio-products. Recently, xylitol has also been used as a drug carrier due to its high permeability and non-toxic nature. However, it become a challenge to fulfil the rapidly increasing market demand of xylitol. Xylitol is present in fruit and vegetables, but at very low concentrations, which is not adequate to satisfy the consumer demand. With the passage of time, other methods including chemical catalysis, microbial and enzymatic biotransformation, have also been developed for its large-scale production. Nevertheless, large scale production still suffers from high cost of production. In this review, we summarize some alternative approaches and recent advancements that significantly improve the yield and lower the cost of production.

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Section: Strain Improvementmentioning

confidence: 99%

Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism

Ahuja

Macho

Ewe

et al. 2020

Foods

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“…AAB strains can also transform d -arabinose to 4-keto- d -arabinose [35] and 4-keto- d -arabonate [36] (Adachi et al, 2010 ); d -ribose to 4-keto- d -ribose [41] and 4-keto- d -ribonate [42] (Adachi et al, 2011a ); xylose to xylonic acid [43] (Buchert et al, 1988 ; Hahn et al, 2020 ), d -xylulose to xylitol [44] (Qi et al, 2016 ), sorbose to sorbitol [45] and 2-keto- d -gulonic acid [46] (Sugisawa et al, 1991 ; Adachi et al, 1999a ), and d -galactose to d -galactonic acid [47] (Švitel and Šturdik, 1994 ). Adachi et al ( 2013 ) also found that AAB cells could use 2-deoxy- d -ribose as a substrate to produce 2-deoxy-4-keto- d -ribose [48] and 2-deoxy-4-keto- d -ribonate [49] , which are under the action of membrane-binding d -aldopentose 4-dehydrogenase and 4-keto- d -1-dehydrogenase, respectively (Adachi et al, 2013 ).…”

Section: Natural Products Yielded By Aofmentioning

confidence: 99%

Oxidative Fermentation of Acetic Acid Bacteria and Its Products

Xie

Zhang

et al. 2022

Front. Microbiol.

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Acetic acid bacteria (AAB) are a group of Gram-negative, strictly aerobic bacteria, including 19 reported genera until 2021, which are widely found on the surface of flowers and fruits, or in traditionally fermented products. Many AAB strains have the great abilities to incompletely oxidize a large variety of carbohydrates, alcohols and related compounds to the corresponding products mainly including acetic acid, gluconic acid, gulonic acid, galactonic acid, sorbose, dihydroxyacetone and miglitol via the membrane-binding dehydrogenases, which is termed as AAB oxidative fermentation (AOF). Up to now, at least 86 AOF products have been reported in the literatures, but no any monograph or review of them has been published. In this review, at first, we briefly introduce the classification progress of AAB due to the rapid changes of AAB classification in recent years, then systematically describe the enzymes involved in AOF and classify the AOF products. Finally, we summarize the application of molecular biology technologies in AOF researches.

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“…Osmophilic yeasts were usually used first for producing D-arabitol from glucose, and then D-arabitol was metabolized to xylitol by Gluconobacter sp. [1,9].…”

Section: Microorganisms With the Ability Of Producing Xylitolmentioning

confidence: 99%

“…Besides, the conversion of D-arabitol to xylitol by ArDH and XDH is the rate-limiting step [14,21,22], therefore, much focus has been attracted into the study in this area. So far, the yield of D-arabitol to xylitol had been achieved more than 83% by genetic engineering strains of BL21-xhd and Gluconobacter oxydans [1], and 87% by genetic engineering strains of BL21-xhd and BL21-xdh through the co-biotransformation of whole cells. The xylitol production was increased by more than two times as compared with that of Gluconobacter sp.…”

Section: Production Of Xylitol By Genetically Engineered Stainsmentioning

confidence: 99%

“…Considering the special anti-caries function, xylitol can be applied as diabetes, nutritional supplements, therapeutic agents and children caries food. In addition, since xylitol also has many excellent properties similar to glycerol and other polyols, it was widely used in the fields of pharmaceutics, chemistry, leather, coatings and food industries [1,2].…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of Xylitol from Glucose: Microorganism, Key Enzymes and Genetically Engineered Strains

Qi¹

2017

BIO

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Xylitol is an important prebiotics, and it is widely used in the fields of medicine and food. In view of the drawbacks of chemical method, much focus has been attracted into the study of xylitol biosynthesis. In this study, microorganism, method and key enzymes in the biosynthesis of xylitol were introduced. In addition, the production of xylitol from glucose by genetically engineered strains was summarized respectively.

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Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell

Cited by 16 publications

References 23 publications

Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism

Biological and Pharmacological Potential of Xylitol: A Molecular Insight of Unique Metabolism

Oxidative Fermentation of Acetic Acid Bacteria and Its Products

Biosynthesis of Xylitol from Glucose: Microorganism, Key Enzymes and Genetically Engineered Strains

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