The specific role of plastidial glycolysis in photosynthetic and heterotrophic cells under scrutiny through the study of glyceraldehyde-3-phosphate dehydrogenase

Anoman, Armand D.; Flores-Tornero, María; Rosa-Téllez, Sara; Muñoz‐Bertomeu, Jesús; Segura, Juan; Ros, Roc

doi:10.1080/15592324.2015.1128614

Cited by 22 publications

(15 citation statements)

References 12 publications

(16 reference statements)

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“…Additionally, studies have shown that GAPCp1 and GAPCp2 are located in the chloroplast in Arabidopsis [ 6 ]. Mutations in the GAPCp gene cause metabolic abnormalities in the triose phosphate transporter (TPT) [ 18 ]. Transcriptome and metabolomic analysis have shown that the lack of GAPCp in plants can disrupt the synthesis of major metabolites such as carbon, nitrogen, glycine and glutamine [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

The Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Is Critical for Abiotic Stress Response in Wheat

Wei

et al. 2019

IJMS

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Plastidial glyceraldehyde-3-phosphate dehydrogenase (GAPDH, GAPCp) are ubiquitous proteins that play pivotal roles in plant metabolism and are involved in stress response. However, the mechanism of GAPCp’s function in plant stress resistance process remains unclear. Here we isolated, identified, and characterized the TaGAPCp1 gene from Chinese Spring wheat for further investigation. Subcellular localization assay indicated that the TaGAPCp1 protein was localized in the plastid of tobacco (Nicotiana tobacum) protoplast. In addition, quantitative real-time PCR (qRT-PCR) unraveled that the expression of TaGAPCp1 (GenBank: MF477938.1) was evidently induced by osmotic stress and abscisic acid (ABA). This experiment also screened its interaction protein, cytochrome b6-f complex iron sulfite subunit (Cyt b6f), from the wheat cDNA library using TaGAPCp1 protein as a bait via the yeast two-hybrid system (Y2H) and the interaction between Cyt b6f and TaGAPCp1 was verified by bimolecular fluorescence complementation assay (BiFC). Moreover, H2O2 could also be used as a signal molecule to participate in the process of Cyt b6f response to abiotic stress. Subsequently, we found that the chlorophyll content in OE-TaGAPCp1 plants was significantly higher than that in wild type (WT) plants. In conclusion, our data revealed that TaGAPCp1 plays an important role in abiotic stress response in wheat and this stress resistance process may be completed by H2O2-mediated ABA signaling pathway.

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Section: Introductionmentioning

confidence: 99%

The Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Is Critical for Abiotic Stress Response in Wheat

Wei

et al. 2019

IJMS

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“…The mechanism by which such alternative C enters isoprenoid biosynthesis is still not fully understood; the observation that the extent of isoprene labelling reflects the labelling of intermediates of the Calvin–Benson cycle may support the view that all carbon in isoprene comes directly out of the cycle (Delwiche and Sharkey, 1993; Sharkey and Monson, 2014). However, alternative carbon sources for isoprene biosynthesis might be cytosolic phospho enol pyruvate (PEP) or pyruvate entering the MEP pathway (Rosenstiel et al , 2004) or other compounds that are directly channeled into the Calvin–Benson cycle after uptake into the chloroplasts, for example as triose-phosphates via P i -transporters (Anoman et al , 2016; de Souza et al , 2018). Under stress conditions that cause reduction of photosynthesis, the portion of leaf internal carbon sources can indeed increase (Kreuzwieser et al , 2002; Funk et al , 2004; Trowbridge et al , 2012).…”

Section: Introductionmentioning

confidence: 99%

Heat stress increases the use of cytosolic pyruvate for isoprene biosynthesis

Yáñez‐Serrano

Mahlau

Fasbender

et al. 2019

Journal of Experimental Botany

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The increasing occurrence of heatwaves has intensified temperature stress on terrestrial vegetation. Here, we investigate how two contrasting isoprene-emitting tropical species, Ficus benjamina and Pachira aquatica, cope with heat stress and assess the role of internal plant carbon sources for isoprene biosynthesis in relation to thermotolerance. To our knowledge, this is the first study to report isoprene emissions from P. aquatica. We exposed plants to two levels of heat stress and determined the temperature response curves for isoprene and photosynthesis. To assess the use of internal C sources in isoprene biosynthesis, plants were fed with 13C position-labelled pyruvate. F. benjamina was more heat tolerant with higher constitutive isoprene emissions and stronger acclimation to higher temperatures than P. aquatica, which showed higher induced isoprene emissions at elevated temperatures. Under heat stress, both isoprene emissions and the proportion of cytosolic pyruvate allocated into isoprene synthesis increased. This represents a mechanism that P. aquatica, and to a lesser extent F. benjamina, has adopted as an immediate response to sudden increase in heat stress. However, in the long run under prolonged heat, the species with constitutive emissions (F. benjamina) was better adapted, indicating that plants that invest more carbon into protective emissions of biogenic volatile organic compounds tend to suffer less from heat stress.

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“…The lack of GAPCp in plants could disrupt the synthesis of major metabolites such as carbon and nitrogen metabolism, glycine and glutamine [5]. Mutations in the GAPCp gene cause metabolic abnormalities in the triose phosphate transporter (TPT) [6]. One of the important functions of GAPCp is to provide 3-phosphoglycerate (3-PGA) for anabolic pathways in heterotrophic cytoplasts [7].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat

Zhang

Song

et al. 2019

BMC Plant Biol

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Background Drought stress is one of the major abiotic stresses that affects plant growth and productivity. The GAPCp genes play important roles in drought stress tolerance in multiple species. The aim of this experiment was to identify the core cis-regulatory elements that may respond to drought stress in the GAPCp2 and GAPCp3 promoter sequences. Results In this study, the promoters of GAPCp2 and GAPCp3 were cloned. The promoter activities were significantly improved under abiotic stress via regulation of Rluc reporter gene expression, while promoter sequence analysis indicated that these fragments were not almost identical. In transgenic Arabidopsis with the expression of the GUS reporter gene under the control of one of these promoters, the activities of GUS were strong in almost all tissues except the seeds, and the activities were induced after abiotic stress. The yeast one-hybrid system and EMSA demonstrated that TaMYB bound TaGAPCp2 P /3 P. By analyzing different 5′ deletion mutants of these promoters, it was determined that TaGAPCp2 P (− 1312~ − 528) and TaGAPCp3 P (− 2049~ − 610), including the MYB binding site, contained enhancer elements that increased gene expression levels under drought stress. We used an effector and a reporter to co-transform tobacco and found that TaMYB interacted with the specific MYB binding sites of TaGAPCp2 P (− 1197~ − 635) and TaGAPCp3 P (− 1456~ − 1144 and − 718~ − 610) in plant cells. Then, the Y1H system and EMSA assay demonstrated that these MYB binding sites in TaGAPCp2 P (− 1135 and − 985) and TaGAPCp3 P (− 1414 and − 665) were the target cis-elements of TaMYB. The deletion of the specific MYB binding sites in the promoter fragments significantly restrained the drought response, and these results confirmed that these MYB binding sites (AACTAAA/C) play vital roles in improving the transcription levels under drought stress. The results of qRT-PCR in wheat protoplasts transiently overexpressing TaMYB indicated that the expression of TaGAPCp2/3 induced by abiotic stress was upregulated by TaMYB. Conclusion The MYB binding sites (AACTAAA/C) in TaGAPCp2 P/ 3 P were identified as the key cis-elements for responding to drought stress and were bound by the transcription factor TaMYB. Electronic supplementary material The online version of this article (10.1186/s12870-019-1948-y) contains supplementary material, which is available to authorized users.

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The specific role of plastidial glycolysis in photosynthetic and heterotrophic cells under scrutiny through the study of glyceraldehyde-3-phosphate dehydrogenase

Cited by 22 publications

References 12 publications

The Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Is Critical for Abiotic Stress Response in Wheat

The Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Is Critical for Abiotic Stress Response in Wheat

Heat stress increases the use of cytosolic pyruvate for isoprene biosynthesis

RETRACTED ARTICLE: The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat

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