RETRACTED ARTICLE: The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat

Abstract

Background Drought stress is one of the major abiotic stresses that affects plant growth and productivity. The GAPCp genes play important roles in drought stress tolerance in multiple species. The aim of this experiment was to identify the core cis-regulatory elements that may respond to drought stress in the GAPCp2 and GAPCp3 promoter sequences. Results In this study, the promoters of GAPCp2 and GAPCp3 were cloned. The promoter activities were significantly improved under abiotic stress via regulation of Rluc reporter gene expression, while promoter sequence analysis indicated that these fragments were not almost identical. In transgenic Arabidopsis with the expression of the GUS reporter gene under the control of one of these promoters, the activities of GUS were strong in almost all tissues except the seeds, and the activities were induced after abiotic stress. The yeast one-hybrid system and EMSA demonstrated that TaMYB bound TaGAPCp2 P /3 P. By analyzing different 5′ deletion mutants of these promoters, it was determined that TaGAPCp2 P (− 1312~ − 528) and TaGAPCp3 P (− 2049~ − 610), including the MYB binding site, contained enhancer elements that increased gene expression levels under drought stress. We used an effector and a reporter to co-transform tobacco and found that TaMYB interacted with the specific MYB binding sites of TaGAPCp2 P (− 1197~ − 635) and TaGAPCp3 P (− 1456~ − 1144 and − 718~ − 610) in plant cells. Then, the Y1H system and EMSA assay demonstrated that these MYB binding sites in TaGAPCp2 P (− 1135 and − 985) and TaGAPCp3 P (− 1414 and − 665) were the target cis-elements of TaMYB. The deletion of the specific MYB binding sites in the promoter fragments significantly restrained the drought response, and these results confirmed that these MYB binding sites (AACTAAA/C) play vital roles in improving the transcription levels under drought stress. The results of qRT-PCR in wheat protoplasts transiently overexpressing TaMYB indicated that the expression of TaGAPCp2/3 induced by abiotic stress was upregulated by TaMYB. Conclusion The MYB binding sites (AACTAAA/C) in TaGAPCp2 P/ 3 P were identified as the key cis-elements for responding to drought stress and were bound by the transcription factor TaMYB. Electronic supplementary material The online version of this article (10.1186/s12870-019-1948-y) contains supplementary material, which is available to authorized users.

Genome-Wide Analysis of the DUF4228 Family in Soybean and Functional Identification of GmDUF4228–70 in Response to Drought and Salt Stresses

“…It has been shown that cis-acting elements in the promoter region are closely related to gene function (Chow et al, 2018). Consistent with their expression profiles, GmDUF4228 genes contained a variety of cisacting elements related to abiotic stresses, including MYB, MYC, MBS, and GT-1 elements (Figure 6), which play an important role in response to drought and salt treatments (Abe et al, 1997;Li et al, 2018;Tang et al, 2019;Zhang L. et al, 2019). Studies have shown that MYC also functions in cold stress (Ohta et al, 2018) and disease resistance (Lorenzo et al, 2004).…”

Genome-wide identification of R2R3-MYB family in wheat and functional characteristics of the abiotic stress responsive gene TaMYB344

“…Previous studies indicate that MYB proteins participate in wheat response to environmental stress. For instance, TaMYB can bind to the specific MYB binding sites in the promoter fragments to upregulate the expression levels of GAPCp2 and GAPCp3, which are involved in drought stress response in wheat [20]. Overexpression of TaMYBsm3-D increases the drought tolerance of transgenic Arabidopsis through upregulating P5CS1, DREB2A, and RD29A [21].…”

Genome-wide analysis of BpDof genes and the tolerance to drought stress in birch (Betula platyphylla)

“…The results ( Fig. 5 ) showed that MYB, MBS, TC-rich, LTR, GT1-motif, G-Box, GA-motif, W-Box, ABRE, ERE, and GARE-motifs, which were related to drought tolerance ( Zhang et al, 2019 ), flavonoid biosynthesis ( Wang et al, 2018 ), defense and stress responsiveness ( Zhang et al, 2005 ), low temperature tolerance ( Feng et al, 2019 ), light response ( Zhang et al, 2013 ; Zhu et al, 2015 ), abiotic stress responsiveness ( Yan et al, 2019 ), abscisic acid responsiveness ( Choi et al, 2005 ), ethylene responsiveness ( Rawat et al, 2005 ), and gibberellin responsiveness, were found in promoters of the 26 BpDof genes. Among these, MYB, MBS, G-Box, GT1-motif, ABRE, and ERE cis-elements were abundant, thus suggesting that these BpDof genes might be involved in drought tolerance, light response, and ABA- and ethylene-responsive signaling.…”