Correction: A Downy Mildew Effector Attenuates Salicylic Acid-Triggered Immunity in Arabidopsis by Interacting with the Host Mediator Complex

Caillaud, Marie‐Cécile; Asai, Shuji; Rallapalli, Ghanasyam; Piquerez, Sophie J. M.; Fabro, Georgina; Jones, Jonathan D. G.

doi:10.1371/journal.pbio.1002408

Cited by 7 publications

(6 citation statements)

References 1 publication

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“…Numerous studies in microbial pathogens have shown that effectors have evolved versatile patterns to target plant cellular processes and suppress plant immunity 48–51 . In this study, we showed that overexpression of Pst_12806 in plants was suppressive to plant basal defense by disturbing the chloroplast function.…”

Section: Discussionmentioning

confidence: 99%

An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function

et al. 2019

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Chloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts and affects chloroplast function. Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat. It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes. Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex). Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS. Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance against Pst infection.

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Section: Discussionmentioning

confidence: 99%

An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function

et al. 2019

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“…SA effectively protects plants from attacks of pathogens, particularly sterol auxotroph oomycetes, by inducing the expression of sterol-binding protein PR1a . SA-responsive genes are upregulated in Arabidopsis by downy mildew, and Arabidopsis mutants lacking the ability to synthesize SA are more sensitive to this pathogen. , The pretreatment of Arabidopsis by SA significantly enhances its resistance to Phytophthora. capsici .…”

Section: Introductionmentioning

confidence: 99%

“…5 SAresponsive genes are upregulated in Arabidopsis by downy mildew, and Arabidopsis mutants lacking the ability to synthesize SA are more sensitive to this pathogen. 6,7 The pretreatment of Arabidopsis by SA significantly enhances its resistance to Phytophthora. capsici.…”

Section: ■ Introductionmentioning

confidence: 99%

Comparative Proteomics Analysis Reveals That Lignin Biosynthesis Contributes to Brassinosteroid-Mediated Response to Phytophthora sojae in Soybeans

Wang¹,

Zhao²,

Guo³

et al. 2020

J. Agric. Food Chem.

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Brassinosteroids (BRs) are a group of steroid plant hormones regulating normal growth, development, and stress response in plants. However, the mechanisms by which BRs interfere with the resistance of soybean to Phytophthora sojae (P. sojae) remain largely unknown. The present study analyzed the role of BRs in soybean response against P. sojae by comparative proteomic approaches. A total of 52,381 peptides were obtained by trypsin digestion of 9,680 proteins, among which 6,640 proteins were quantified, and 402 proteins were identified as differentially expressed proteins (DEPs). Further analysis revealed that DEPs were significantly involved in the lignin biosynthesis pathway. The expression of the majority of key enzymes involved in lignin biosynthesis was upregulated by BR-pretreatment and P. sojae infection, and lignin accumulation was faster in BR-pretreated soybeans than in untreated controls. Additionally, accumulation of lignin was consistent with these enzyme expressions levels and resistance phenotype. These findings advance the understanding of the role of BRs in the interaction between soybeans and P. sojae.

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“…Salicylic acid signaling is an integral part of plant defense responses and has been demonstrated to be involved in defense against downy mildews and other biotrophic pathogens (Delaney Terrence P. et al, 1994;Mohr et al, 2010). Pathogen virulence strategies have developed to overcome and inhibit salicylic acid defenses, thus enhancing susceptibility to biotrophic pathogens such as downy mildew organisms (Caillaud et al, 2013(Caillaud et al, , 2016. This suggests a likely role of salicylic acid signaling in MRI BDM resistance, though the specific mechanisms of signaling induction remain unknown.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel basil downy mildew resistance genes using de novo comparative transcriptomics

Allen

DeIulio

Pyne

et al. 2022

Preprint

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Basil downy mildew (BDM), caused by the oomycete pathogen Peronospora belbahrii, is the most significant disease threatening sweet basil production across the world. A downy mildew resistant sweet basil cultivar Mrihani (MRI) was identified in a germplasm screen, and fertile progeny were produced through a breeding program with BDM-susceptible commercial sweet basil cultivar Newton (SB22). However, the specific genes and molecular mechanisms which confer the resistance in MRI and selected progeny remain unknown. This study was designed to identify candidate resistance genes and the potential mechanisms that enable BDM resistance through a comparative transcriptomic approach. RNA samples from BDM-infected MRI and SB22 plants were harvested at 4 time points during the first 3 days of infection to capture genes, pathways, and biological processes involved in disease resistance. This global transcription expression profile confirmed the continuous growth of the pathogen only in the susceptible cultivar SB22, consistent with the infection phenotype. Three categories of genes uniquely induced in the MRI cultivar upon pathogen challenge were identified: the plant pathogen recognition nucleotide-binding leucine rich repeat proteins (NLRs), the multi-functional receptor-like kinases, and secondary metabolic enzymes. Validation of the top resistance candidate NLR gene confirmed its unique presence in the MRI cultivar as well as two out of four resistant MRIxSB22 F2 progeny. Additionally, unique upregulation of the salicylic acid synthesis pathway genes in MRI suggests the importance of this hormone signaling pathway in BDM resistance. This study demonstrates the feasibility of using comparative transcriptomics to identify resistance genes in non-model crops for rapid improvement in marker-assisted breeding approaches.

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Correction: A Downy Mildew Effector Attenuates Salicylic Acid-Triggered Immunity in Arabidopsis by Interacting with the Host Mediator Complex

Cited by 7 publications

References 1 publication

An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function

An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function

Comparative Proteomics Analysis Reveals That Lignin Biosynthesis Contributes to Brassinosteroid-Mediated Response to Phytophthora sojae in Soybeans

Identification of novel basil downy mildew resistance genes using de novo comparative transcriptomics

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