Comparative Proteomics Analysis Reveals That Lignin Biosynthesis Contributes to Brassinosteroid-Mediated Response to <i>Phytophthora sojae</i> in Soybeans

Wang, Xinfang; Zhao, Zisu; Guo, Na; Wang, Hai-Tang; Zhao, Jinming

doi:10.1021/acs.jafc.0c00848

Cited by 11 publications

(9 citation statements)

References 47 publications

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“…Other studies, using proteomic approaches, have focused on the exogenous application of BRs and their capacity to mediate response to P. sojae in soybean. BRs significantly enriched the lignin biosynthesis, which was consistent with the resistance phenotype [75].…”

Section: Molecular Response Of Soybean To Phytophthora Sojaesupporting

confidence: 74%

“…Although these MM are still used in classic genetic breeding programs, the new technologies of massive DNA sequencing (next-generation sequencing NGS) have become the main strategy for the discovery of single nucleotide polymorphisms (SNPs) and genotyping in large populations. Among the used methods are the restriction site-associated DNA tag sequencing (RADseq), genotyping by-sequencing (GBS), 2b-RAD, and specific length amplified fragment sequencing (SLAF-seq) [75].…”

Section: Host Resistancementioning

confidence: 99%

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Phytophthora Root Rot: Importance of the Disease, Current and Novel Methods of Control

2022

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Phytophthora sojae is a pathogen of major agricultural importance, responsible for Phytophthora root rot (PRR) in soybean crops, which can cause significant yield losses each year. The severity of the disease depends on the soybean cultivar, its growth stage at the time of pathogen infection, and the environmental conditions. High soil moisture and temperature around 25–30 °C are favorable conditions for the development of the disease. Consequently, cultural practices are mainly limited to avoiding bad weather (high moisture) during the sowing or to promoting soil drainage. The use of chemical fungicides is restricted to seed treatments when there is a high risk of disease development. Currently the most economical option for controlling P. sojae is the use of host resistance. However, even if breeding is the main control strategy of PRR, the use of resistant cultivars leads to selection pressure on P. sojae populations, which can lead to high variability of the pathogen and therefore to its adaptation to overcome plant resistance. New strategies are therefore needed, including the use of biological control agents (BCAs). The use of BCAs (i.e., microorganisms or their metabolites) is a promising and sustainable alternative to PRR control that should be strengthened. Therefore, this review addresses the P. sojae–soybean interaction, mechanisms of pathogenicity and host resistance, as well as current and new management strategies with emphasis on the biological control of P. sojae and its associated mechanisms.

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Section: Molecular Response Of Soybean To Phytophthora Sojaesupporting

confidence: 74%

Section: Host Resistancementioning

confidence: 99%

Phytophthora Root Rot: Importance of the Disease, Current and Novel Methods of Control

2022

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“…A protein involved in lignin synthesis (glyma18g38670.1) was also found. Lignins were found to participate in cell wall reinforcement upon oomycete P. sojae infection [ 78 , 79 ].…”

Section: Resultsmentioning

confidence: 99%

Elicitation of Roots and AC-DC with PEP-13 Peptide Shows Differential Defense Responses in Multi-Omics

Chambard

Mlouka

Jing³

et al. 2022

Cells

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The root extracellular trap (RET) has emerged as a specialized compartment consisting of root AC-DC and mucilage. However, the RET’s contribution to plant defense is still poorly understood. While the roles of polysaccharides and glycoproteins secreted by root AC-DC have started to be elucidated, how the low-molecular-weight exudates of the RET contribute to root defense is poorly known. In order to better understand the RET and its defense response, the transcriptomes, proteomes and metabolomes of roots, root AC-DC and mucilage of soybean (Glycine max (L.) Merr, var. Castetis) upon elicitation with the peptide PEP-13 were investigated. This peptide is derived from the pathogenic oomycete Phytophthora sojae. In this study, the root and the RET responses to elicitation were dissected and sequenced using transcriptional, proteomic and metabolomic approaches. The major finding is increased synthesis and secretion of specialized metabolites upon induced defense activation following PEP-13 peptide elicitation. This study provides novel findings related to the pivotal role of the root extracellular trap in root defense.

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“…The key P450 families participating in these phytoalexin biosynthesis belong to CYP71. Similarly, phytohormones, such as brassinosteroid, jasmonic acid, ABA, and GA also provide defense against biotic and abiotic stress ( Bulgakov et al, 2019 ; Kohli et al, 2019 ; Yang et al, 2019 ; Wang et al, 2020 ) and involve P450 enzymes for their biosynthesis. The production of phytoalexin glyceollins upon exposure of soybean to environmental challenges has been long known.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Cytochrome P450 Monooxygenases Reveals Insight Into Their Role in Partial Resistance Against Phytophthora sojae in Soybean

et al. 2022

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Cytochrome P450 monooxygenases (P450) participate in the catalytic conversion of biological compounds in a plethora of metabolic pathways, such as the biosynthesis of alkaloids, terpenoids, phenylpropanoids, and hormones in plants. Plants utilize these metabolites for growth and defense against biotic and abiotic stress. In this study, we identified 346 P450 (GmP450) enzymes encoded by 317 genes in soybean where 26 GmP450 genes produced splice variants. The genome-wide comparison of both A-type and non-A-type GmP450s for their motifs composition, gene structure, tissue-specific expression, and their chromosomal distribution were determined. Even though conserved P450 signature motifs were found in all GmP450 families, larger variation within a specific motif was observed in the non-A-type GmP450s as compared with the A-type. Here, we report that the length of variable region between two conserved motifs is exact in the members of the same family in majority of the A-type GmP450. Analyses of the transcriptomic datasets from soybean-Phytophthora sojae interaction studies, quantitative trait loci (QTL) associated with P. sojae resistance, and co-expression analysis identified some GmP450s that may be, in part, play an important role in partial resistance against P. sojae. The findings of our CYPome study provides novel insights into the functions of GmP450s and their involvements in metabolic pathways in soybean. Further experiments will elucidate their roles in general and legume-specific function.

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Comparative Proteomics Analysis Reveals That Lignin Biosynthesis Contributes to Brassinosteroid-Mediated Response to Phytophthora sojae in Soybeans

Cited by 11 publications

References 47 publications

Phytophthora Root Rot: Importance of the Disease, Current and Novel Methods of Control

Phytophthora Root Rot: Importance of the Disease, Current and Novel Methods of Control

Elicitation of Roots and AC-DC with PEP-13 Peptide Shows Differential Defense Responses in Multi-Omics

Comprehensive Analysis of Cytochrome P450 Monooxygenases Reveals Insight Into Their Role in Partial Resistance Against Phytophthora sojae in Soybean

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