Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses

Radauer-Preiml, Isabella; Andosch, Ancuela; Hawranek, Thomas; Luetz-Meindl, Ursula; Wiederstein, Markus; Horejs‐Hoeck, Jutta; Himly, Martin; Boyles, Matthew; Duschl, Albert

doi:10.1186/s12989-016-0113-0

Cited by 56 publications

(47 citation statements)

References 79 publications

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“…Therefore, studies on the interaction of nanomaterials with cells and biomolecules can open new avenues in understanding the structural characteristic required for the bio-affinity of NPs toward pharmacological activity. [1][2][3][4] Nanomaterials can demonstrate a strong influence on cell viability and structural and corresponding functional features of biomolecules. Therefore, continuous interest has been focused toward comprehending the fundamental effects of NPs for interaction with biological systems.…”

Section: Introductionmentioning

confidence: 99%

Biophysical, docking, and cellular studies on the effects of cerium oxide nanoparticles on blood components: in vitro

Eskandari¹,

Babadaei²,

Nikpur³

et al. 2018

IJN

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IntroductionThe application of nanoparticles (NPs) in medicine and biology has received great interest due to their novel features. However, their adverse effects on the biological system are not well understood.Materials and methodsThis study aims to evaluate the effect of cerium oxide nanoparticles (CNPs) on conformational changes of human hemoglobin (HHb) and lymphocytes by different spectroscopic (intrinsic and synchronous fluorescence spectroscopy and far and near circular dichroism [CD] spectroscopy), docking and cellular (MTT and flow cytometry) investigations.Results and discussionTransmission electron microscopy (TEM) showed that CNP diameter is ~30 nm. The infrared spectrum demonstrated a strong band around 783 cm−1 corresponding to the CNP stretching bond. Fluorescence data revealed that the CNP is able to quench the intrinsic fluorescence of HHb through both dynamic and static quenching mechanisms. The binding constant (Kb), number of binding sites (n), and thermodynamic parameters over three different temperatures indicated that hydrophobic interactions might play a considerable role in the interaction of CNPs with HHb. Synchronous fluorescence spectroscopy indicated that microenvironmental changes around Trp and Tyr residues remain almost unchanged. CD studies displayed that the regular secondary structure of HHb had no significant changes; however, the quaternary structure of protein is subjected to marginal structural changes. Docking studies showed the larger CNP cluster is more oriented toward experimental data, compared with smaller counterparts. Cellular assays revealed that CNP, at high concentrations (>50 µg/mL), initiated an antiproliferative response through apoptosis induction on lymphocytes.ConclusionThe findings may exhibit that, although CNPs did not significantly perturb the native conformation of HHb, they can stimulate some cellular adverse effects at high concentrations that may limit the medicinal and biological application of CNPs. In other words, CNP application in biological systems should be done at low concentrations.

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Section: Introductionmentioning

confidence: 99%

Biophysical, docking, and cellular studies on the effects of cerium oxide nanoparticles on blood components: in vitro

Eskandari¹,

Babadaei²,

Nikpur³

et al. 2018

IJN

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“…To date, there has been no report on the allergy‐enhancing effect of silver NPs. Very recently, it was reported that when Der p 1, an allergen closely related to that used in our study, was bound to nanogold, IgE‐dependent basophil degranulation and the proteolytic activity were increased …”

Section: Resultsmentioning

confidence: 54%

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

Kang

Kim

Lee

et al. 2016

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The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.

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“…The formation of unique protein coronas based on the capping material has been reported to influence the inflammatory response to AgNP by modulating the way nanomaterials interact with cells or tissues [56–58]. One study of gold nanoparticles revealed different capping agents have the ability to elicit differential effects on both in vitro and in vivo toxicity [59].…”

Section: Discussionmentioning

confidence: 99%

Impact of pulmonary exposure to gold core silver nanoparticles of different size and capping agents on cardiovascular injury

et al. 2015

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BackgroundThe uses of engineered nanomaterials have expanded in biomedical technology and consumer manufacturing. Furthermore, pulmonary exposure to various engineered nanomaterials has, likewise, demonstrated the ability to exacerbate cardiac ischemia reperfusion (I/R) injury. However, the influence of particle size or capping agent remains unclear. In an effort to address these influences we explored response to 2 different size gold core nanosilver particles (AgNP) with two different capping agents at 2 different time points. We hypothesized that a pulmonary exposure to AgNP induces cardiovascular toxicity influenced by inflammation and vascular dysfunction resulting in expansion of cardiac I/R Injury that is sensitive to particle size and the capping agent.MethodsMale Sprague–Dawley rats were exposed to 200 μg of 20 or 110 nm polyvinylprryolidone (PVP) or citrate capped AgNP. One and 7 days following intratracheal instillation serum was analyzed for concentrations of selected cytokines; cardiac I/R injury and isolated coronary artery and aorta segment were assessed for constrictor responses and endothelial dependent relaxation and endothelial independent nitric oxide dependent relaxation.ResultsAgNP instillation resulted in modest increase in selected serum cytokines with elevations in IL-2, IL-18, and IL-6. Instillation resulted in a derangement of vascular responses to constrictors serotonin or phenylephrine, as well as endothelial dependent relaxations with acetylcholine or endothelial independent relaxations by sodium nitroprusside in a capping and size dependent manner. Exposure to both 20 and 110 nm AgNP resulted in exacerbation cardiac I/R injury 1 day following IT instillation independent of capping agent with 20 nm AgNP inducing marginally greater injury. Seven days following IT instillation the expansion of I/R injury persisted but the greatest injury was associated with exposure to 110 nm PVP capped AgNP resulted in nearly a two-fold larger infarct size compared to naïve.ConclusionsExposure to AgNP may result in vascular dysfunction, a potentially maladaptive sensitization of the immune system to respond to a secondary insult (e.g., cardiac I/R) which may drive expansion of I/R injury at 1 and 7 days following IT instillation where the extent of injury could be correlated with capping agents and AgNP size.Electronic supplementary materialThe online version of this article (doi:10.1186/s12989-016-0159-z) contains supplementary material, which is available to authorized users.

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Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses

Cited by 56 publications

References 79 publications

Biophysical, docking, and cellular studies on the effects of cerium oxide nanoparticles on blood components: in vitro

Biophysical, docking, and cellular studies on the effects of cerium oxide nanoparticles on blood components: in vitro

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

Impact of pulmonary exposure to gold core silver nanoparticles of different size and capping agents on cardiovascular injury

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