5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

Abstract

The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.

Biogenic Silver Nanoparticles as a Post-surgical Treatment for Corynebacterium pseudotuberculosis Infection in Small Ruminants

“…Pulit et al (2011) cited that the contact of humans with AgNPs may cause adverse effects, and these different results may have been due to the different composition and thickness of the skin of small ruminants and humans. Another aspect to be considered is the size of the AgNPs, since Kang et al (2017) described that 5 nm AgNPs amplify the clinical features of atopic dermatitis, and Park et al (2011) found that 4 nm AgNPs presented a higher cytotoxic effect than AgNPs at high concentrations with 20 and 70 nm sizes. The AgNPs studied herein have an average size of 28 nm, and this characteristic can then be associated with a lower occurrence of adverse effects.…”

Metal nanomaterials: Immune effects and implications of physicochemical properties on sensitization, elicitation, and exacerbation of allergic disease

“…Aluminum nanoparticles (AlNP), AuNP, FeNP, and SiNP have all been shown to modify DC antigen cross-presentation capacity (Blank et al 2011; Li et al 2011; Hirai et al 2012; Jiménez-Periáñez et al 2013; Kang S et al 2017; Mou et al 2017; Dong et al 2018). The mecha-nism of antigen uptake by DC is known to influence the preferential association of antigens with MHC I or II molecules.…”

“…This effect was shown to be more pronounced with decreasing size with respect to AgNP and SiO 2 NP, but not for TiO 2 NP (Yanagisawa et al 2009; Hirai et al 2012b; Hirai et al 2015). Exposure to 5 nm AgNP during sensitization was associated with an augmentation of mast cell activity that resulted in more severe skin lesions that appeared earlier than those induced by 100 nm AgNP (Kang H et al 2017). Decreases in SiO 2 NP size were also associated with enhanced T H 2 responses, as evidenced by increased thymic stromal lymphopoeitin (TSLP) and IL-18 production (Hirai et al 2012b).…”

Nanotechnology meets atopic dermatitis: Current solutions, challenges and future prospects. Insights and implications from a systematic review of the literature

“…Despite their hope and promises, nanotechnology-based solution can have also triggering effect on the induction of allergic reactions and the clinical amplification of such symptoms. Utilizing a mouse model, Kang and collaborators [86] found that 5- but not 100-nm nano-sized Ag-NPs induce release of granule from tryptase-positive mast cells, as well as lead to increased reactive oxygen species (ROS, hydrogen peroxide and mitochondrial superoxide) levels and intracellular calcium concentrations.…”