Multidrug resistance in Clostridium perfringens isolated from diarrheal neonatal piglets in Thailand

Ngamwongsatit, Bhinyada; Tanomsridachchai, Wimonrat; Suthienkul, Orasa; Urairong, Supanee; Navasakuljinda, Wichian; Janvilisri, Tavan

doi:10.1016/j.anaerobe.2015.12.012

Cited by 28 publications

(14 citation statements)

References 19 publications

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“…The dominance of type A isolates in both manure and digestate is in agreement with the high prevalence of this toxinotype in farm animals [38][39][40]. Although the cpe gene was not detected in either manure or digestate, the three BGPs produced digestate containing virulent C. perfringens.…”

Section: Toxinotyping Of C Perfringens Isolatessupporting

confidence: 76%

Characterization of Clostridium Perfringens Isolates Collected from Three Agricultural Biogas Plants over a One-Year Period

Derongs

Druilhe

Ziebal

et al. 2020

IJERPH

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Digestate produced by agricultural biogas plants (BGPs) may contain pathogenic bacteria. Among them, Clostridium perfringens deserves particular attention due to its ability to grow under anaerobic conditions and persist in amended soil. The aim of this study was to examine the potential pathogenicity and the antimicrobial resistance of C. perfringens in manure and digestate collected from three agricultural biogas plants (BGPs). A total of 157 isolates (92 from manure, 65 from digestate) were screened for genes encoding seven toxins (cpa, cpb, etx, iapcpe, netB, and cpb2). The 138 cpa positive isolates were then screened for tetA(P), tetB(P), tet(M), and erm(Q) genes and tested for antimicrobial susceptibility. The toxinotypes identified in both manure and digestate were type A (78.3% of the isolates), type G (16.7%), type C (3.6%), and type D (1.4%), whereas none of the isolates were type F. Moreover, half of the isolates carried the cpb2 gene. The overall prevalence of tetA(P) gene alone, tetA(P)-tetB(P) genes, and erm(Q) gene was 31.9, 34.8, and 6.5%, respectively. None of the isolates harbored the tet(M) gene. Multiple antimicrobial resistant isolates were found in samples that were collected from all the manure and digestates. Among them, 12.3% were highly resistant to some of the antibiotics tested, especially to clindamycin (MIC ≥ 16 µg/mL) and tilmicosin (MIC > 64 µg/mL). Some isolates were highly resistant to antibiotics used in human medicine, including vancomycin (MIC > 8 µg/mL) and imipenem (MIC > 64 µg/mL). These results suggest that digestate may be a carrier of the virulent and multidrug resistant C. perfringens.

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Section: Toxinotyping Of C Perfringens Isolatessupporting

confidence: 76%

Characterization of Clostridium Perfringens Isolates Collected from Three Agricultural Biogas Plants over a One-Year Period

Derongs

Druilhe

Ziebal

et al. 2020

IJERPH

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“…Macrolide and Lincosamide resistance (mainly erythromycin and lincomycin) appears widespread 86 , and therefore is considered ineffective in treating C. perfringens infections. This is supported by a recent multidrug-resistance study of 260 strains of C. perfringens isolated from diarrheal neonatal piglets in Thailand, where higher resistance was observed for erythromycin, lincomycin and tylosin 87 .…”

Section: Antimicrobial Resistance (Amr)mentioning

confidence: 61%

An update on the human and animal enteric pathogen Clostridium perfringens

Kiu

Hall

2018

Emerging Microbes & Infections

315

285

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Clostridium perfringens, a rapid-growing pathogen known to secrete an arsenal of >20 virulent toxins, has been associated with intestinal diseases in both animals and humans throughout the past century. Recent advances in genomic analysis and experimental systems make it timely to re-visit this clinically and veterinary important pathogen. This Review will summarise our understanding of the genomics and virulence-linked factors, including antimicrobial potentials and secreted toxins of this gut pathogen, and then its up-to-date clinical epidemiology and biological role in the pathogenesis of several important human and animal-associated intestinal diseases, including pre-term necrotising enterocolitis. Finally, we highlight some of the important unresolved questions in relation to C. perfringens-mediated infections, and implications for future research directions.

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“…Three publications have investigated AMR in poultry pathogens, including Mycoplasma gallisepticum , Haemophilus paragallinarum , and Avibacterium paragallinarum [95,96,97], two in clinical and animal Burkholderia pseudomallei isolates [98,99], and one in Clostridium perfringens bacteria from diarrhoeic pigs [100]. …”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

et al. 2016

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Southeast Asia is an area of great economic dynamism. In recent years, it has experienced a rapid rise in the levels of animal product production and consumption. The region is considered to be a hotspot for infectious diseases and antimicrobial resistance (AMR). We reviewed English-language peer-reviewed publications related to antimicrobial usage (AMU) and AMR in animal production, as well as antimicrobial residues in meat and fish from 2000 to 2016, in the region. There is a paucity of data from most countries and for most bacterial pathogens. Most of the published work relates to non-typhoidal Salmonella (NTS), Escherichia coli (E. coli), and Campylobacter spp. (mainly from Vietnam and Thailand), Enterococcus spp. (Malaysia), and methicillin-resistant Staphylococcus aureus (MRSA) (Thailand). However, most studies used the disk diffusion method for antimicrobial susceptibility testing; breakpoints were interpreted using Clinical Standard Laboratory Institute (CSLI) guidelines. Statistical models integrating data from publications on AMR in NTS and E. coli studies show a higher overall prevalence of AMR in pig isolates, and an increase in levels of AMR over the years. AMU studies (mostly from Vietnam) indicate very high usage levels of most types of antimicrobials, including beta-lactams, aminoglycosides, macrolides, and quinolones. This review summarizes information about genetic determinants of resistance, most of which are transferrable (mostly plasmids and integrons). The data in this review provide a benchmark to help focus research and policies on AMU and AMR in the region.

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Multidrug resistance in Clostridium perfringens isolated from diarrheal neonatal piglets in Thailand

Cited by 28 publications

References 19 publications

Characterization of Clostridium Perfringens Isolates Collected from Three Agricultural Biogas Plants over a One-Year Period

Characterization of Clostridium Perfringens Isolates Collected from Three Agricultural Biogas Plants over a One-Year Period

An update on the human and animal enteric pathogen Clostridium perfringens

Antimicrobial Usage and Antimicrobial Resistance in Animal Production in Southeast Asia: A Review

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