Detection of the emerging rotavirus G12P[8] genotype at high frequency in brazil in 2014: Successive replacement of predominant strains after vaccine introduction

Luchs, Adriana; Cilli, Audrey; Morillo, Simone Guadagnucci; Gregorio, D. de Souza; Souza, Karen Aparecida Farias de; Vieira, Heloisa Rosa; Fernandes, Adeline de Mira; Carmona, Rita de Cássia Compagnoli; Timenetsky, María do Carmo Sampaio Tavares

doi:10.1016/j.actatropica.2015.12.008

Cited by 23 publications

(20 citation statements)

References 59 publications

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“…In this study, RVA genotype G12P[8] was the prevalent genotype, corroborating previous reports conducted in Asian, European, American, and African continents, demonstrating the epidemiological relevance of this genotype worldwide [Cunliffe et al, ; Page et al, ; Potgieter et al, ; Jere et al, ; Page et al, ; Seheri et al, ; Bucardo et al, ; Luchs et al, ]. The high proportion of genotype G12 in African populations is believed to be associated with the emergence of genotype P[6] [Steele and Ivanoff, ; Page et al, ; Seheri et al, ].…”

Section: Discussionsupporting

confidence: 88%

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Langa

Thompson²,

Arnaldo³

et al. 2016

Journal of Medical Virology

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Acute diarrhea disease caused by Rotaviruses A (RVA) is still the leading cause of morbidity and mortality in children ≤5 years old in developing countries. An exploratory cross-sectional study was conducted between February and September, 2011 to determine the proportion of acute diarrhea caused by RVA. A total of 254 stool specimens were collected from children ≤5 years old with acute diarrhea, including outpatients (222 children) and inpatients (32 children), in three local health centers in Chókwè District, Gaza Province, South of Mozambique. RVA antigens were detected using enzyme immunoassay (EIA); the RVA G (VP7) and P (VP4) genotypes were determined by RT-PCR or analysis sequencing. Sixty (24%) out of 254 fecal specimens were positive for RVA by EIA; being 58 (97%) from children ≤2 years of age. RVA prevalence peaks in June and July (coldest and drier months) and the G[P] binary combination observed were G12P[8] (57%); G1P[8] (9%); G12P[6] (6%); and 2% for each of the following genotypes: G1P[6], G2P[6] G4P[6], and G9P[8]. Non-Typeable (NT) G and/or P genotypes were observed as follows: G12P [NT] (6%); G1P [NT], G3P[NT] and GNTP[NT] (4%). Considering the different GP combinations, G12 represented 67% of the genotypes. This is the first data showing the diversity of RVA genotypes in Mozambique highlighting the epidemiological importance of these viruses in acute diarrhea cases in children ≤2 years old. In addition, these findings will provide a baseline data before the introduction of the RVA monovalent (Rotarix(®) ) vaccine in the National Immunization Program in September 2015. J. Med. Virol. 88:1751-1758, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 88%

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Langa

Thompson²,

Arnaldo³

et al. 2016

Journal of Medical Virology

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“…Considering the reduced RVA detection rate in Brazil after the introduction of RV1: 18% [Carvalho‐Costa et al, Personal communication] and 19.7% [Luchs et al, ], the present results revealed an important increase of DD related to G12P[8] RVA in Brazil in 2014. The increase of the G12P[8] prevalence started in the beginning of June, the dry season in Brazil and RVA seasonality, coincided with the FIFA Football World Cup period held in all five Brazilian regions from June, 11th to July 13th, 2014.…”

Section: Resultsmentioning

confidence: 57%

“…The monthly G12P[8] detection and distribution is shown in Figure . Previous studies reported the circulation of G12 genotype in Brazil [Pietruchinski et al, ; Soares et al, ; Gomez et al, ; Luchs et al, ; Neves et al, ].…”

Section: Resultsmentioning

confidence: 99%

“…Studies reporting analysis of G12P[8] strains show the wide circulation of the G12‐III lineage in the last years, suggesting that this lineage is associated with the global spread of RVA genotype G12 worldwide, considered the 6th most prevalent human RVA genotype nowadays. On the other hand, other G12 lineages (as G12‐I and G12‐II) remain sporadically detected and usually detected in association with other RVA P genotypes [Delogu et al, ; Tort et al, ; Langa et al, ; Luchs et al, ].…”

Section: Resultsmentioning

confidence: 99%

“…RVA accounted for 24.3% of DD cases in Latin America and the Caribbean Region (LAC) [Linhares et al, ; Lanata et al, ]. However, comparing pre‐ and post‐vaccination periods, the RVA prevalence decreased significantly demonstrating the efficacy of RVA vaccine programs in the NIP of the LAC region [Carvalho‐Costa et al, Personal communication; de Oliveira et al, ; Luchs et al, ].…”

Section: Introductionmentioning

confidence: 99%

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VP7 and VP8* genetic characterization of group A rotavirus genotype G12P[8]: Emergence and spreading in the Eastern Brazilian coast in 2014

Silva

Fumian

Assis

et al. 2016

Journal of Medical Virology

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Group A rotavirus (RVA) genotype G12 is habitually associated with diarrhea disease (DD) in African children and recently its detection has increased worldwide. A total of 970 stool samples collected from individuals with DD in the Northeastern, Southeastern, and Southern Brazilian regions, Eastern coast, were analyzed and 321 (33%) were positive for RVA and of these, 241 (75%) genotyped as G12P[8]. The rate of RVA positivity was higher among children aged 5-10 years old (60%). All RVA infections observed in adults aged >21 years were G12P[8] (n = 27) showing that this genotype affected older age groups during the year of 2014 in Brazil. Phylogenetic analysis of VP7 and VP8* G12P[8] strains demonstrated an elevated similarity among Brazilian and G12-III prototypes strains circulating worldwide recently, suggesting that this lineage is associated with the global spread of the G12 genotype, considered as the 6th most prevalent human RVA genotype nowadays; while other G12 lineages remain sporadically detected and usually detected in association with other P genotypes. VP8* analysis revealed that Brazilian strains belong to P[8]-3 lineage, the single P[8] lineage presently detected in the country. No major nucleotide/amino acid disparities were observed among strains recovered from children and adults for VP7 and VP8* genes. These data are essential to support the surveillance studies, particularly in countries where the RVA vaccine was introduced in their National Immunization Program enabling identification of potential alterations in the epidemiological profile that can impact its efficacy in vaccination programs. J. Med. Virol. 89:64-70, 2017. © 2016 Wiley Periodicals, Inc.

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Molecular Epidemiology of Human Rotaviruses in Latin America

Degiuseppe

Stupka

Parra

2017

Human Virology in Latin America

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Detection of the emerging rotavirus G12P[8] genotype at high frequency in brazil in 2014: Successive replacement of predominant strains after vaccine introduction

Cited by 23 publications

References 59 publications

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

VP7 and VP8* genetic characterization of group A rotavirus genotype G12P[8]: Emergence and spreading in the Eastern Brazilian coast in 2014

Molecular Epidemiology of Human Rotaviruses in Latin America

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