Neuroprotective effects of ginseng protein on PI3K/Akt signaling pathway in the hippocampus of D -galactose/AlCl 3 inducing rats model of Alzheimer’s disease

Li, Hongyan; Kang, Ting-Guo; Qi, Bin; Kong, Liang; Jiao, Yanan; Cao, Yang; Zhang, Jianghua; Yang, Jingxian

doi:10.1016/j.jep.2015.12.020

Cited by 94 publications

(51 citation statements)

References 51 publications

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“…In the behavioral study, chronic D-gal and AlCl 3 administration led to the bad performance of mice in the Morris water maze test and step-though test, which was consistent with previous reports [38, 39]. Morris water maze tasks could reflect spatial memory ability of animal sensitively.…”

Section: Discussionsupporting

confidence: 90%

The Protective Effect of Lavender Essential Oil and Its Main Component Linalool against the Cognitive Deficits Induced by D‐Galactose and Aluminum Trichloride in Mice

Wang

Lü

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Lavender essential oil (LO) is a traditional medicine used for the treatment of Alzheimer's disease (AD). It was extracted from Lavandula angustifolia Mill. This study was designed to investigate the effects of lavender essential oil (LO) and its active component, linalool (LI), against cognitive impairment induced by D-galactose (D-gal) and AlCl3 in mice and to explore the related mechanisms. Our results revealed that LO (100 mg/kg) or LI (100 mg/kg) significantly protected the cognitive impairments as assessed by the Morris water maze test and step-though test. The mechanisms study demonstrated that LO and LI significantly protected the decreased activity of superoxide dismutase (SOD), glutathione peroxidase (GPX), and protected the increased activity of acetylcholinesterase (AChE) and content of malondialdehyde (MDA). Besides, they protected the suppressed nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression significantly. Moreover, the decreased expression of synapse plasticity-related proteins, calcium-calmodulin-dependent protein kinase II (CaMKII), p-CaMKII, brain-derived neurotrophic factor (BDNF), and TrkB in the hippocampus were increased with drug treatment. In conclusion, LO and its active component LI have protected the oxidative stress, activity of cholinergic function and expression of proteins of Nrf2/HO-1 pathway, and synaptic plasticity. It suggest that LO, especially LI, could be a potential agent for improving cognitive impairment in AD.

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Section: Discussionsupporting

confidence: 90%

The Protective Effect of Lavender Essential Oil and Its Main Component Linalool against the Cognitive Deficits Induced by D‐Galactose and Aluminum Trichloride in Mice

Wang

Lü

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…For example, red ginseng root extract protected skin from acute UVB irradiation [4], and ginsenoside Rb1 promotes the healing process of burn wounds by enhancing angiogenesis [5]. Recently, protein from ginseng was studied and used for treatment of several kinds of diseases [6], [7], [8], [9], [10]. However, the effect of ginseng total protein (GTP) on proliferation and wound healing in fibroblast cells remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Chen

Wang

et al. 2017

Journal of Ginseng Research

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BackgroundRecently, protein from ginseng was studied and used for the treatment of several kinds of diseases. However, the effect of ginseng total protein (GTP) on proliferation and wound healing in fibroblast cells remains unclear.MethodsIn this study, cell viability was analyzed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Cell cycle distribution was analyzed by flow cytometer. The levels of transforming growth factor β1, vascular endothelial growth factor, and collagens were analyzed by enzyme-linked immunosorbent assay and immunofluorescence staining. The expressions of cyclin A, phosphorylation of extracellular signal-related kinase (p-ERK1/2), and ERK1/2 were analyzed by Western blotting.ResultsOur results showed that GTP promoted cell proliferation and increased the percentage of cells in S phase through the upregulation of cyclin A in NIH/3T3 cells. We also found that GTP induced the secretion of type I collagen, and promoted the expression of other factors that regulate the synthesis of collagen such as transforming growth factor β1 and vascular endothelial growth factor. In addition, the phosphorylation of ERK1/2 at Thr202/Tyr204 was also increased by GTP.ConclusionOur studies suggest that GTP promoted proliferation and secretion of collagen in NIH/3T3 cells by activating the ERK signal pathway, which shed light on a potential function of GTP in promoting wound healing.

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“…However, ICA pretreatment ameliorated this effect, further confirming that ICA protects against NaN 3induced PC12 cell damage through inhibition of mitochondrial dysfunction caused by glucose metabolism disorder. The PI3K/Akt/GSK-3β signaling pathway plays an important role in the development, survival and function of neurons (Li et al, 2016;Zhang et al, 2016), which is critical for the regulation of mitochondrial function. We hypothesized that ICA improves brain mitochondrial energy metabolism disorder, and its mechanism is related to the PI3K/Akt/ Figure 6 Effect of ICA on the phosphorylation level of Tau in NaN 3 -injured PC12 cells.…”

Section: Discussionmentioning

confidence: 99%

Icariin protects against sodium azide—induced neurotoxicity by activating the PI3K/Akt/GSK-3β signaling pathway

Zhang

Huang

et al. 2020

PeerJ

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Background Icariin (ICA) is one of the major active flavonoids extracted from the traditional Chinese herb Epimedium brevicornum Maxim and has been shown to have neuroprotective effects. This study was designed to investigate the effect of ICA on sodium azide (NaN3)-induced rat adrenal pheochromocytoma (PC12) cell damage and to further examine the underlying mechanisms. Methods To explore its possible mechanism, we used NaN3 (50 mM)-induced neuronal PC12 cell damage. Cell viability was evaluated by CCK-8 and lactate dehydrogenase (LDH) assays. Mitochondrial membrane potential (MMP) was detected by JC-1. Glucose concentration was assessed by the glucose oxidase method. The role of ICA in the PI3K/Akt/GSK-3β signaling pathway was explored by Western blotting. Results The results indicate that pretreatment with ICA reduced NaN3-induced cell damage and significantly reduced the leakage rate of LDH in PC12 cells. ICA pretreatment increased the MMP and a decrease in glucose concentration indicate increased glucose consumption. Furthermore, the protein levels of p-PI3K (p85), PI3K-110α, p-Ser473-Akt and p-Ser9-GSK-3β were markedly decreased in PC12 cells after NaN3 treatment for 24 h, whereas these effects were reverted after pretreatment with ICA. Tau phosphorylation at the Ser396/404 and Thr217 sites was significantly decreased by pretreatment with ICA. Conclusions These results suggest that ICA protects against NaN3-induced neurotoxicity in PC12 cells by activating the PI3K/Akt/GSK-3β signaling pathway.

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Neuroprotective effects of ginseng protein on PI3K/Akt signaling pathway in the hippocampus of D -galactose/AlCl 3 inducing rats model of Alzheimer’s disease

Cited by 94 publications

References 51 publications

The Protective Effect of Lavender Essential Oil and Its Main Component Linalool against the Cognitive Deficits Induced by D‐Galactose and Aluminum Trichloride in Mice

The Protective Effect of Lavender Essential Oil and Its Main Component Linalool against the Cognitive Deficits Induced by D‐Galactose and Aluminum Trichloride in Mice

Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Icariin protects against sodium azide—induced neurotoxicity by activating the PI3K/Akt/GSK-3β signaling pathway

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