Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Chen, Xuenan; Wang, Man-Ying; Xu, Xiaohao; Liu, Jianzeng; Mei, Bing; Zhao, Daqing; Sun, Liwei

doi:10.1016/j.jgr.2017.02.001

Cited by 28 publications

(16 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Rb1 supplementation improved the blastocyst quality by anti‐apoptosis and induced pluripotency gene expressions. These effects of Rb1 on apoptosis and stem cell proliferation are similar to the results of previous studies 43‐45 . Ginseng can be converted into more easily absorbed ginsenoside after oral administration 46 .…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos

Luo

Xuan

Han

et al. 2020

Environmental Toxicology

View full text Add to dashboard Cite

Panax ginseng, a functional food, has been widely used as an edible nourishment and medicinal supplement. Ginsenoside Rb1 is a major bioactive ingredient of ginseng, which shows very specific anti‐apoptosis and anti‐oxidant activities. Methylglyoxal (MGO) is one of intermediate products of glucose metabolism, which is absorbed easily from high sugar foods or carbonated beverages. It may involve in a variety of detrimental processes in vivo. However, it has not been fully explored the effects of ginsenoside Rb1 on MGO‐induced oocytes damage. This study found that MGO‐induced DNA damage and mitochondrial dysfunction result in the failure of porcine oocytes maturation and low in vitro development capacity of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos. Conversely, Rb1 supplementation recovered the rate of maturation, and improved in vitro development capacity of PA and IVF embryos. Rb1 also provided porcine oocytes a lower level of reactive oxygen species production, higher level of ATP content and mitochondrial membrane potential, and stimulated pluripotency gene expression in blastocysts. The findings of this study reveal ginsenoside Rb1 protects porcine oocyte from the cytotoxicity effects of methylglyoxal and provides novel perspectives for the protection of reproduction system by functional food of ginseng.

show abstract

Section: Discussionsupporting

confidence: 88%

“…These effects of Rb1 on apoptosis and stem cell proliferation are similar to the results of previous studies. [43][44][45] Ginseng can be converted into more easily absorbed ginsenoside after oral administration. 46…”

Section: Rb1 Improved In Vitro Development Of Ivf Embryosmentioning

confidence: 99%

Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos

Luo

Xuan

Han

et al. 2020

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…The voucher specimen (2145) was conserved at the Herbarium of College of Science, Beihua University (Jilin province, China). GP was prepared by the same method in the published article of the same laboratory [24]. Fresh ginseng roots (1 kg) were cut into small slices and extracted twice with phosphate-buffered saline (PBS) at 4°C for 4 h. The supernatant was condensed with an ultrafiltration membrane (30 kDa) and the concentrated solution was separated with Sephadex G50, the purity of which reached 90.2%.…”

Section: Methodsmentioning

confidence: 99%

Panax ginseng Total Protein Facilitates Recovery from Dexamethasone-Induced Muscle Atrophy through the Activation of Glucose Consumption in C2C12 Myotubes

Jiang

Wang

Shi

et al. 2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Background. The clinical anti-inflammatory drug dexamethasone (DEX) can cause many side effects such as muscle atrophy for long-term use. Muscle atrophy induced by DEX may be caused by decrease of glucose consumption. Panax ginseng C.A. Meyer was previously considered to be an antiatrophic agent for glucocorticoid- (GC-) treated therapies. As one of the main components, it remains unclear whether ginseng total protein (GP) facilitates recovery from muscle atrophy induced by DEX. Methods. In this study, GP was extracted and purified with Sephadex-G50. C2C12 myoblasts was induced with 2% horse serum to differentiate into C2C12 myotubes. Cell viability was analyzed by the MTT assay, and Ca2+ concentration was analyzed by a flow cytometer. The release of lactic dehydrogenase (LDH) and the glucose consumption were analyzed by spectrophotometry. The phosphorylation of AMP-activated protein kinase (AMPK), phosphoinositide 3-kinase (PI3K), and protein kinase B (Akt) and the expression of glucose transporter 4 (GLUT4) were analyzed by Western blotting. The phosphorylation of AS160 was quantified by Immunofluorescence staining. Results. We found that GP increased cell viability and increased myotube diameter in high-dose DEX-treated C2C12 myotubes for 24 h, but this activity was not found in the enzymatic hydrolyzed GP group. GP reduced muscle atrophy by decreasing the expression of key proteins such as muscle RING-finger protein-1 and muscle atrophy F-box, reducing the Ca2+ concentration, and decreasing the release of LDH in DEX-injured C2C12 myotubes. Moreover, GP improved glucose consumption and increased the phosphorylation of AMPK, PI3K, Akt, and AS160 and the expression of GLUT4 in DEX-treated C2C12 myotubes. Conclusion. The results of this study suggest that GP has effects on recovering DEX-induced muscle atrophy and cell injury, which may improve glucose consumption via the AMPK and PI3K/Akt pathways in high-dose DEX-treated C2C12 myotubes. This study provides in vitro mechanistic insights into the recovery of muscle atrophy with GP treatment.

show abstract

“…The root extracts of Panax ginseng have been shown to protect skin in C57BL mice from acute UVB irradiation [233] and significantly improve healing after laser burn injury and excisional wounding [221, 234, 235]. Studies demonstrate Panax ginseng extracts enhance keratinocyte migration [221, 236], as well as stimulate proliferation [237] and increase collagen synthesis in human dermal fibroblasts [238] in vitro . In addition, Choi demonstrated that the ginsenoside Rb2, isolated from Panax ginseng , induces the formation of the epidermis in raft culture via increased expression of epidermal growth factor and its receptor, fibronectin and its receptor, and keratin 5/14 and collagenase I [239], all of which have critical roles in wound healing.…”

Section: Traditional Use Of Medical Plants In Wound Healingmentioning

confidence: 99%

Wound Healing and the Use of Medicinal Plants

Shedoeva

Leavesley

Upton

et al. 2019

Evidence-Based Complementary and Alternative Medicine

268

164

View full text Add to dashboard Cite

Cutaneous wound healing is the process by which skin repairs itself. It is generally accepted that cutaneous wound healing can be divided into 4 phases: haemostasis, inflammation, proliferation, and remodelling. In humans, keratinocytes re-form a functional epidermis (reepithelialization) as rapidly as possible, closing the wound and reestablishing tissue homeostasis. Dermal fibroblasts migrate into the wound bed and proliferate, creating “granulation tissue” rich in extracellular matrix proteins and supporting the growth of new blood vessels. Ultimately, this is remodelled over an extended period, returning the injured tissue to a state similar to that before injury. Dysregulation in any phase of the wound healing cascade delays healing and may result in various skin pathologies, including nonhealing, or chronic ulceration. Indigenous and traditional medicines make extensive use of natural products and derivatives of natural products and provide more than half of all medicines consumed today throughout the world. Recognising the important role traditional medicine continues to play, we have undertaken an extensive survey of literature reporting the use of medical plants and plant-based products for cutaneous wounds. We describe the active ingredients, bioactivities, clinical uses, formulations, methods of preparation, and clinical value of 36 medical plant species. Several species stand out, including Centella asiatica, Curcuma longa, and Paeonia suffruticosa, which are popular wound healing products used by several cultures and ethnic groups. The popularity and evidence of continued use clearly indicates that there are still lessons to be learned from traditional practices. Hidden in the myriad of natural products and derivatives from natural products are undescribed reagents, unexplored combinations, and adjunct compounds that could have a place in the contemporary therapeutic inventory.

show abstract

Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Cited by 28 publications

References 43 publications

Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos

Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos

Panax ginseng Total Protein Facilitates Recovery from Dexamethasone-Induced Muscle Atrophy through the Activation of Glucose Consumption in C2C12 Myotubes

Wound Healing and the Use of Medicinal Plants

Contact Info

Product

Resources

About