Antimicrobial and cell viability measurement of bovine serum albumin capped silver nanoparticles (Ag/BSA) loaded collagen immobilized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film

Bakare, Rotimi; Hawthrone, Samantha; Vails, Carmen; Gugssa, Ayele; Karim, Alamgir; Stubbs, John T.; Raghavan, Dharmaraj

doi:10.1016/j.jcis.2015.11.041

Cited by 24 publications

(7 citation statements)

References 36 publications

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“…The viability of MES23.5 cells was measured 24 h following the transfection of Hapln2 plasmid using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay as described previously (Bakare et al, 2015). Briefly, MTT solution (5 mg/ml) was added to the cells in 96-well plate containing 150 μl of DMEM and incubated for 4 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Elevated Hapln2 Expression Contributes to Protein Aggregation and Neurodegeneration in an Animal Model of Parkinson's Disease

Wang

Zhou

Zhang

et al. 2016

Front. Aging Neurosci.

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Parkinson's disease (PD), the second most common age-associated progressive neurodegenerative disorder, is characterized by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SN). The pathogenesis of PD and the mechanisms underlying the degeneration of DA neurons are still not fully understood. Our previous quantitative proteomics study revealed that hyaluronan and proteoglycan binding link protein 2 (Hapln2) is one of differentially expressed proteins in the substantia nigra tissues from PD patients and healthy control subjects. However, the potential role of Hapln2 in PD pathogenesis remains elusive. In the present study, we characterized the expression pattern of Hapln2. In situ hybridization revealed that Hapln2 mRNA was widely expressed in adult rat brain with high abundance in the substantia nigra. Immunoblotting showed that expression levels of Hapln2 were markedly upregulated in the substantia nigra of either human subjects with Parkinson's disease compared with healthy control. Likewise, there were profound increases in Hapln2 expression in neurotoxin 6-hydroxydopamine-treated rat. Overexpression of Hapln2 in vitro increased vulnerability of MES23.5 cells, a dopaminergic cell line, to 6-hydroxydopamine. Moreover, Hapln2 overexpression led to the formation of cytoplasmic aggregates which were co-localized with ubiquitin and E3 ligases including Parkin, Gp78, and Hrd1 in vitro. Endogenous α-synuclein was also localized in Hapln2-containing aggregates and ablation of Hapln2 led to a marked decrease of α-synuclein in insoluble fraction compared with control. Thus, Hapln2 is identified as a novel factor contributing to neurodegeneration in PD. Our data provides new insights into the cellular mechanism underlying the pathogenesis in PD.

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Section: Methodsmentioning

confidence: 99%

Elevated Hapln2 Expression Contributes to Protein Aggregation and Neurodegeneration in an Animal Model of Parkinson's Disease

Wang

Zhou

Zhang

et al. 2016

Front. Aging Neurosci.

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“…When the studies on the production of scaffolds by various methods using Ag nanoparticles in their structures, which were designed for use in bone tissue cells, were investigated, it was observed that cytotoxicity was related to silver concentration . For example, while Marsich et al and Bakare et al found the 0.05% silver ratio for their bone tissue structure composites, Hasan et al, Alt et al and Gao et al stated that 1% and 2.5% Ag content suitable for bone tissue applications. Based on these data, the nanocomposite structure obtained in this study has the potential to be used in bone tissue applications.…”

Section: Resultsmentioning

confidence: 99%

Optimization of the electrospinning process variables for gelatin/silver nanoparticles/bioactive glass nanocomposites for bone tissue engineering

2020

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The current research focuses on the findings of an investigation on optimizing of an electrospun antibacterial gelatin nanocomposite membrane for bone tissue engineering applications. For this reason, soluble starch coated silver nanoparticles (Ag‐NPs) and bioactive glass particles (BG) were incorporated in to gelatin (Gt) to fabricate Gt/Ag‐NPs/BG nanocomposite membranes. Employing Box‐Behnken design, second‐order models have been successfully obtained to evaluate the statistical significance of individual and interaction effects of applied voltage, tip‐to‐collector distance (TCD), and flow rate on fiber diameter. There was a reasonable agreement between the regression R2 value (0.9542), R2 predicted value (0.8768), and the R2 adjusted value (0.9286) across the entire factor space with identical observations for the experimental and model values. Under optimum conditions (applied voltage of 26 kV, TCD of 180 mm, and flow rate of 0.5 mL/h), the nanocomposite membrane with similar fiber size of bone tissue extracellular matrix can be fabricated with the predicted value of 557 nm obtained by the proposed model. The optimized nanofiber membrane was fabricated under these conditions and average fiber diameter of this membrane was found as 472 ± 94 nm. The characterization studies of this nanofiber suggest that obtained nanocomposite is a potential candidate for bone tissue engineering applications.

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“…Some nanoparticles may have antibacterial activity, and affect many species of bacteria (Lu et al, 2013;Huang et al, 2015;Bakare et al, 2016). For example, copper nanoparticles have been proved as promising antibacterial agents against the intestinal bacteria Aeromonas hydrophila, Vibrio parahaemolyticus, and Pseudomonas fluorescens in silver and grass carp (Huang et al, 2015), and Kim et al (2007) reported that the use of silver nanoparticles (AgNPs) inhibited the growth of yeast, Escherichia coli (Escherich), and Staphylococcus aureus (Rosenbach).…”

Section: _____________________mentioning

confidence: 99%

Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP B)

Acedo-Valdez

Chon

Larios-Rodríguez

et al. 2017

lajar

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ABSTRACT. The shrimp necrotizing hepatopancreatitis is caused by a pleomorphic Gram-negative Rickettsialike bacteria known as NHP-B. It is well known that silver is an effective bactericidal, but experiments with aquatic organisms are scarce, even more at nanoparticle level. The objective of this study was to determine the antibacterial effect of silver nanoparticles (AgNP) against NHP-B in the Pacific white shrimp Litopenaeus vannamei. For this, AgNO3 was used as source of silver, and extracts of dried leaves of green tea Camellia sinensis and neem tree Azadirachta indica as reducing agents. Different lots of infected shrimp received 0,5 and 35 μg of AgNP by forced feeding. The differences between the number of bacterial nodules in shrimp hepatopancreas and shrimp mortality relative to the amount of AgNP demonstrated to be effective against this pathogen.

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Antimicrobial and cell viability measurement of bovine serum albumin capped silver nanoparticles (Ag/BSA) loaded collagen immobilized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film

Cited by 24 publications

References 36 publications

Elevated Hapln2 Expression Contributes to Protein Aggregation and Neurodegeneration in an Animal Model of Parkinson's Disease

Elevated Hapln2 Expression Contributes to Protein Aggregation and Neurodegeneration in an Animal Model of Parkinson's Disease

Optimization of the electrospinning process variables for gelatin/silver nanoparticles/bioactive glass nanocomposites for bone tissue engineering

Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP B)

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