Description of Campylobacter jejuni Bf, an atypical aero-tolerant strain

Rodrigues, Ramila Cristiane; Pocheron, Anne-Lise; Hernould, Michel; Haddad, Nabila; Tresse, Odile; Cappelier, Jean‐Michel

doi:10.1186/s13099-015-0077-x

Cited by 38 publications

(56 citation statements)

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“…In addition, Rodrigues et al (2015) recently characterized an unique human isolate of C. jejuni strain, named Bf, which can grow aerobically, suggesting that some C. jejuni strains are highly resistant to aerobic stress. Increased tolerance to aerobic stress would enable C. jejuni to survive during transmission to humans through foods.…”

Section: Discussionmentioning

confidence: 99%

Differential Survival of Hyper-Aerotolerant Campylobacter jejuni under Different Gas Conditions

McMullen

Chui

et al. 2017

Front. Microbiol.

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Campylobacter jejuni accounts for a significant number of foodborne illnesses around the world. C. jejuni is microaerophilic and typically does not survive efficiently in oxygen-rich conditions. We recently reported that hyper-aerotolerant (HAT) C. jejuni are highly prevalent in retail poultry meat. To assess the capabilities of HAT C. jejuni in foodborne transmission and infection, in this study, we investigated the prevalence of virulence genes in HAT C. jejuni and the survival in poultry meat in atmosphere at a refrigeration temperature. When we examined the prevalence of eight virulence genes in 70 C. jejuni strains from raw poultry meat, interestingly, the frequencies of detecting virulence genes were significantly higher in HAT C. jejuni strains than aerosenstive C. jejuni strains. This suggests that HAT C. jejuni would potentially be more pathogenic than aerosensitive C. jejuni. Under aerobic conditions, aerosensitive C. jejuni survived at 4°C in raw poultry meat for 3 days, whereas HAT C. jejuni survived in poultry meat for a substantially extended time; there was a five-log CFU reduction over 2 weeks. In addition, we measured the effect of other gas conditions, including N2 and CO2, on the viability of HAT C. jejuni in comparison with aerosensitive and aerotolerant strains. N2 marginally affected the viability of C. jejuni. However, CO2 significantly reduced the viability of C. jejuni both in culture media and poultry meat. Based on the results, modified atmosphere packaging using CO2 may help us to control poultry contamination with HAT C. jejuni.

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Section: Discussionmentioning

confidence: 99%

Differential Survival of Hyper-Aerotolerant Campylobacter jejuni under Different Gas Conditions

McMullen

Chui

et al. 2017

Front. Microbiol.

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“…Rodrigues et al (2015) recently reported the aerobic growth of C. jejuni strain Bf on Karmali agar. For this strain visible growth was reported within 24 h of aerobic incubation at 42°C.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to C. coli OR12 Aer, growth in liquid medium could not be elicited, and a quantitative assay was used to measure growth on solid medium. Superior resistance to peroxide stress was also noted compared to C. jejuni NCTC 11168, which had failed to grow aerobically (Rodrigues et al, 2015). Growth on solid medium creates oxygen gradients, such that facultative anaerobes have been found to be more active on the bottom layer and aerobes more rapidly dividing on the top layer of colonies (Reyrolle and Letellier, 1979).…”

Section: Discussionmentioning

confidence: 99%

“…Resistance to peroxide stress was performed using a method similar to that of Rodrigues et al (2015). Glass universal containers, filled with 10 ml of MRD containing 0, 0.5 or 1 mM H 2 O 2 (Sigma; UK), were inoculated with identical optical densities of approximately 10 8 CFU/ml microaerobically grown C. coli RM2228 , C. coli OR12 WT , C. coli OR12 Aer P40 and aerobically grown C. coli OR12 Aer P40.…”

Section: Methodsmentioning

confidence: 99%

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Characterisation of Aerotolerant Forms of a Robust Chicken Colonizing Campylobacter coli

O'Kane

Connerton

2017

Front. Microbiol.

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Campylobacter contaminated poultry meat is a major source of human foodborne illness. Campylobacter coli strain OR12 is a robust colonizer of chickens that was previously shown to outcompete and displace other Campylobacter strains from the chicken’s gastrointestinal tract. This strain is capable of aerobic growth on blood agar. Serial aerobic passage increased this aerotolerance as assessed by quantitative assays for growth and survival on solid media. Aerotolerance was also associated with increased peroxide stress resistance. Aerobic passage did not alter cellular morphology or motility or hinder the microaerobic growth rate. Colonization of broiler chickens by aerotolerant C. coli OR12 was significantly lower than the wild-type strain at 3 days after challenge but not by 7 days, suggesting adaptation had occurred. Bacteria recovered from chickens had retained their aerotolerance, indicating this trait is stable. Whole genome sequencing enabled comparison with the wild-type sequence. Twenty-three point mutations were present, none of which were in genes known to affect oxidative stress resistance. Insertions or deletions caused frame shifts in several genes including, phosphoglycerate kinase and the b subunit of pyruvate carboxylase that suggest modification of central and carbohydrate metabolism in response to aerobic growth. Other genes affected include those encoding putative carbonic anhydrase, motility accessory factor, filamentous haemagglutinin, and aminoacyl dipeptidase proteins. Aerotolerance has the potential to affect environmental success and survival. Increased environmental survival outside of the host intestinal tract may allow opportunities for transmission between hosts. Resistance to oxidative stress may equate to increased virulence by virtue of reduced susceptibility to oxidative free radicals produced by host immune responses. Finally, resistance to ambient atmospheric oxygen may allow increased survival on chicken skin, and therefore constitutes an increased risk to public health.

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“…However, some strains are able to better counteract aerobic conditions for their survival. Recently, the atypical C. jejuni Bf was described to be able to grow under aerobic conditions in contrast to other C. jejuni (Rodrigues et al, 2015, 2016). As for C. jejuni Bf, C. coli OR12 is also able to develop an habituation to aerobic conditions.…”

Section: Introductionmentioning

confidence: 99%